Maize grain yield response to changes in acid soil characteristics with yearly leguminous crop rotation, fallow, slash, burn and liming practices

Open Access JournalAn experiment was conducted for 4 years to assess the effectiveness of fallow, slash and burn farming systems on maize grain yield and soil chemical characteristics. It was also meant to measure the response to yearly rotation of maize and leguminous crops (cowpea and mucuna), as options for managing the acidity of the soil of the study site. The maize tolerant cultivar (cvr) out yielded the sensitive cvr and the farmers’ variety by 43% and 16% respectively. On the maize/grain legume rotation plots, the tolerant and sensitive cvr yielded 5% and 7% respectively more than their corresponding yields on plots with fallow, slash and burn rotation. Maize/grain legume rotation demonstrated one of the least soil acidifications, exhibiting the least increase in exchangeable Al (23%), H (24%), and Al saturation (5%) resulting in improved soil fertility through increase in available Ca (2%), Mg (85%), P (75%), and CEC (14%). The fallow, slash and burn rotation, associated with the tolerant cvr showed similar grain yield with grain legume rotation, but contributed more to soil acidification. Maize/leafy legume rotation gave a similar yield to the above mentioned practices. The yearly application of 250 kg ha-1 of dolomitic lime for four consecutive years did not result in significant changes in soil characteristics and grain yield especially for the Al tolerant cvr. However, application of 2250 kg ha-1 of lime neutralized the Al toxicity, regardless of the rotation scheme. The study concluded that the four years maize cultivation through fallow/ slash and burn rotation extensively used in the humid forest zone is not the best option on acid soil

Genomic analyses of Burkholderia cenocepacia reveal multiple species with differential host-adaptation to plants and humans

Background Burkholderia cenocepacia is a human opportunistic pathogen causing devastating symptoms in patients suffering from immunodeficiency and cystic fibrosis. Out of the 303 B. cenocepacia strains with available genomes, the large majority were isolated from a clinical context. However, several isolates originate from other environmental sources ranging from aerosols to plant endosphere. Plants can represent reservoirs for human infections as some pathogens can survive and sometimes proliferate in the rhizosphere. We therefore investigated if B. cenocepacia had the same potential. Results We selected genome sequences from 31 different strains, representative of the diversity of ecological niches of B. cenocepacia, and conducted comparative genomic analyses in the aim of finding specific niche or host-related genetic determinants. Phylogenetic analyses and whole genome average nucleotide identity suggest that strains, registered as B. cenocepacia, belong to at least two different species. Core-genome analyses show that the clade enriched in environmental isolates lacks multiple key virulence factors, which are conserved in the sister clade where most clinical isolates fall, including the highly virulent ET12 lineage. Similarly, several plant associated genes display an opposite distribution between the two clades. Finally, we suggest that B. cenocepacia underwent a host jump from plants/environment to animals, as supported by the phylogenetic analysis. We eventually propose a name for the new species that lacks several genetic traits involved in human virulence. Conclusion Regardless of the method used, our studies resulted in a disunited perspective of the B. cenocepacia species. Strains currently affiliated to this taxon belong to at least two distinct species, one having lost several determining animal virulence factors

Maize Grain Yield Response to Changes in Acid Soil Characteristics with Yearly Leguminous Crop Rotation, Fallow, Slash, Burn and Liming Practices

An experiment was conducted for 4 years to assess the effectiveness of fallow, slash and burn farming systems on maize grain yield and soil chemical characteristics. It was also meant to measure the response to yearly rotation of maize and leguminous crops (cowpea and mucuna), as options for managing the acidity of the soil of the study site. The maize tolerant cultivar (cvr) out yielded the sensitive cvr and the farmers’ variety by 43% and 16% respectively. On the maize/grain legume rotation plots, the tolerant and sensitive cvr yielded 5% and 7% respectively more than their corresponding yields on plots with fallow, slash and burn rotation. Maize/grain legume rotation demonstrated one of the least soil acidifications, exhibiting the least increase in exchangeable Al (23%), H (24%), and Al saturation (5%) resulting in improved soil fertility through increase in available Ca (2%), Mg (85%), P (75%), and CEC (14%). The fallow, slash and burn rotation, associated with the tolerant cvr showed similar grain yield with grain legume rotation, but contributed more to soil acidification. Maize/leafy legume rotation gave a similar yield to the above mentioned practices. The yearly application of 250 kg ha-1 of dolomitic lime for four consecutive years did not result in significant changes in soil characteristics and grain yield especially for the Al tolerant cvr. However, application of 2250 kg ha-1 of lime neutralized the Al toxicity, regardless of the rotation scheme. The study concluded that the four years maize cultivation through fallow/ slash and burn rotation extensively used in the humid forest zone is not the best option on acid soil

Detection of a novel intracellular microbiome hosted in arbuscular mycorrhizal fungi

Arbuscular mycorrhizal fungi (AMF) are important members of the plant microbiome. They are obligate biotrophs that colonize the roots of most land plants and enhance host nutrient acquisition. Many AMF themselves harbor endobacteria in their hyphae and spores. Two types of endobacteria are known in Glomeromycota: rod-shaped Gram-negative Candidatus Glomeribacter gigasporarum, CaGg, limited in distribution to members of the Gigasporaceae family, and coccoid Mollicutes-related endobacteria, Mre, widely distributed across different lineages of AMF. The goal of the present study is to investigate the patterns of distribution and coexistence of the two endosymbionts, CaGg and Mre, in spore samples of several strains of Gigaspora margarita. Based on previous observations, we hypothesized that some AMF could host populations of both endobacteria. To test this hypothesis, we performed an extensive investigation of both endosymbionts in G. margarita spores sampled from Cameroonian soils as well as in the Japanese G. margarita MAFF520054 isolate using different approaches (molecular phylotyping, electron microscopy, fluorescence in situ hybridization and quantitative real-time PCR). We found that a single AMF host can harbour both types of endobacteria, with Mre population being more abundant, variable and prone to recombination than the CaGg one. Both endosymbionts seem to retain their genetic and lifestyle peculiarities regardless of whether they colonize the host alone or together. These findings show for the first time that fungi support an intracellular bacterial microbiome, in which distinct types of endobacteria coexist in a single cell