Improving Colon Cancer Screening in a Resident Ambulatory Clinic

AIM Statement: Our aim at Wednesday JHAP was to increase the rate of colorectal cancer screenings from 44.3% to 60% from September 2016 to May 2017 (9 months)https://jdc.jefferson.edu/patientsafetyposters/1032/thumbnail.jp

Morphometric relationships between length-weight and length-length and condition factor of four small indigenous fishes from the Payra River, southern Bangladesh

The present study describes the length–weight (LWR) relationship, length– length (LLR) relationship, and condition factor (K) of four small indigenous fish species from the Payra River, southern Bangladesh, namely Mastacembelus pancalus, Lepidocephalus guntea, Salmostoma bacaila and Mystus vittatus. A total of 867 specimens, representing 4 species of 4 families used for this study were caught by traditional fishing gear from July to October 2018. Standard length (SL) and total length (TL) for each specimen were measured by digital slide calipers and each body weight (BW) was taken by a digital balance. The allometric coefficient b of the LWR was close to the isometric value (b=3.078 and 3.028) in M. pancalus and L. guntea respectively, although it suggested negative allometric growth in M. vittatus (b < 3.00), whilst positive allometric growth in S. bacaila (b > 3.00). All the LWRs were highly significant (P < 0.05) and most of the coefficients of determination values were ≥ 0.857. The results further indicated that the LLRs were highly correlated (r2 ≥ 0.939; P < 0.05). Fulton’s condition factor (K) by month basis ranged from 0.52 (in M. pancalus) through 1.89 (in M. vittatus). The results of this study can be very effective for stock assessment of this four species in Payra River as well as in the surrounding ecosystems

Growth and yield of short duration Aman rice (Oryza sativa) cultivars as influenced by age of seedlings

An experiment was conducted at the Agronomy Field Laboratory, Bangladesh Agricultural University (BAU), Mymensingh during the period from July to December 2019 to study the effect of cultivar and seedling age on the performance of short duration transplant Aman rice. The experiment comprised four Aman rice cultivars, viz., BRRI dhan49, BRRI dhan56, BRRI dhan66 and BRRI dhan71, and four seedling ages viz. 20, 25, 30 and 35-day old seedlings. The experiment was laid out in a randomized complete block design with three replications. Results of the study showed that growth, yield and yield contributing characters were significantly influenced by cultivars, seedlings age and their interactions. At growth stage, BRRI dhan49 with 20-day old seedlings produced the tallest plant (57.67 cm and 67.33 cm, respectively), the highest number of total tillers hill-1 (15.00 and 13.67, respectively) and total dry matter (8.03 g m-2 and 11.50 g m-2, respectively) at 30 and 50 DAT. At harvest, the highest number of total and effective tillers hill-1 (12.82 and 12.00), longest panicle (24.50 cm), highest number of grains panicle-1 (128.80), heaviest 1000-grain weight (23.17 g), highest grain yield (5.35 t ha-1) and highest harvest index (51.69 %) were obtained from the cultivar BRRI dhan66. While, thirty-day old seedlings produced the highest number of total and effective tillers hill-1 (13.46 and 12.70), longest panicle (24.67 cm), highest number of grains panicle-1 (136.90), highest grain (5.62 t ha-1) and straw yields (5.81 t ha-1) and harvest index (51.67 %). In case of interactions, BRRI dhan66 with 30-day old seedlings produced the highest number of total and effective tillers hill-1 (14.67 and 13.97), longest panicle (26.00 cm), highest number of grains panicle-1 (146.7), highest grain yield (6.31 t ha-1) and highest harvest index (52.72 %). So, result of the present study reveals that BRRI dhan66 with 30-days old seedlings was found to be the best for obtaining maximum grain yield

Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis.

Here, we describe a rapid and versatile protocol to generate gapped DNA substrates for single-molecule (SM) analysis using optical tweezers via site-specific Cas9 nicking and force-induced melting. We provide examples of single-stranded (ss) DNA gaps of different length and position. We outline protocols to visualize these substrates by replication protein A-enhanced Green Fluorescent Protein (RPA-eGFP) and SYTOX Orange staining using commercially available optical tweezers (C-TRAP). Finally, we demonstrate the utility of these substrates for SM analysis of bidirectional growth of RAD-51-ssDNA filaments. For complete details on the use and execution of this protocol, please refer to Belan et al. (2021)

Response of integrated fertilizer and weed management on weed occurrence and growth traits of aromatic Boro rice

An experiment was conducted at the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh to study the effect of integrated fertilizer and weed management on the growth performance of aromatic Boro rice (cv. BRRI dhan50). The experiment comprised six fertilizer managements viz., control (no manures and no fertilizers), recommended dose of inorganic fertilizers (i.e. Urea, TSP, MoP, Gypsum, ZnSO4 @ 250, 120, 120, 100, 10 kg ha-1, respectively), 50% of recommended dose of inorganic fertilizers + cowdung @ 5 t ha-1, 75% of recommended dose of inorganic fertilizers + cowdung @ 5 t ha-1, 50% of recommended dose of inorganic fertilizers + poultry manure @ 2.5 t ha-1, and 75% of recommended dose of inorganic fertilizers +  poultry manure @ 2.5 t ha-1 and four weed managements viz., control (no weeding), pre-emergence herbicide, Panida 33 EC @ 2.5 l ha-1 + one hand weeding at 35 DAT, post-emergence herbicide, Granite 240 SC @ 93.70 ml ha-1 + one hand weeding at 35 DAT, pre-emergence herbicide, Panida 33 EC @ 2.5 l ha-1 + post-emergence herbicide, Granite 240 SC @ 93.70 ml ha-1. The experiment was laid out in a factorial randomized complete block design with three replications. Growth traits of aromatic Boro rice (cv. BRRI dhan50) were significantly influenced by integrated fertilizer and weed management. Plant height, number of tillers hill-1, total dry matter, leaf area index (LAI) and crop growth rate (CGR) gave their highest values in 75% of recommended dose of inorganic fertilizers and poultry manure @ 2.5 t ha-1 along with pre-emergence herbicide, Panida 33 EC @ 2.5 l ha-1 + post-emergence herbicide, Granite 240 SC @ 93.70 ml ha-1 while their corresponding lowest values were found in control. So it can be concluded that, the interaction of 75% of recommended dose of inorganic fertilizers and poultry manure @ 2.5 t ha-1 along with pre-emergence herbicide (Panida 33 EC @ 2.5 l ha-1) + post-emergence herbicide (Granite 240 SC @ 93.70 ml ha-1) appears as the promising combination in respect of growth performance of aromatic Boro rice (cv. BRRI dhan50)

Integrated nutrient management practices affect the growth performance of aromatic fine rice varieties in Boro season

The experiment was conducted at the Agronomy Field Laboratory, Bangladesh Agricultural University during November 2016 to April 2017 to investigate the effect of nutrient management on the growth performance of some aromatic rice varieties in Boro season. The experiment was laid out in a randomized complete block design with three replications considering two factors viz. varieties and nutrient management. This experiment consisted of three varieties viz., BRRI dhan50, Basmati and BRRI dhan63, and seven nutrient managements viz., poultry manure 5 t ha-1, recommended dose of chemical fertilizers (i.e. 250, 126, 120, 100 and 10 kg N-P-K-S-Zn, respectively ha-1), 25% less than recommended dose of chemical fertilizer + poultry manure @ 2.5 t ha-1, 50% less than recommended dose of chemical fertilizer + poultry manure @ 5 t ha-1, vermicompost @ 10 t ha-1, 25% less than recommended dose of chemical fertilizer + vermicompost @ 5 t ha-1 and 50% less than recommended dose of chemical fertilizer + vermicompost @ 10 t ha-1. The results revealed that variety, nutrient management and their interaction exerted significant influence on growth characters of aromatic fine rice in Boro season. The highest plant height (55.14 cm, 66.14 cm) at 70 and 85 DAT, dry matter hill-1 (15.39 g) at 85 DAT and chlorophyll content (39.29) at 55 DAT was recorded from BRRI dhan63. While, Basmati produced the highest leaf area index (0.60) at 60 DAT and the highest number of total tillers hill-1 (11.52, 10.81, 9.619) was obtained from BRRI dhan50 at 55, 70, 85 DAT. In case of nutrient management practices, 25% less than recommended dose of chemical fertilizer + vermicompost 5 t ha-1 produced the highest plant height (45.22cm, 55.22cm, 65.67 cm) at 55, 70, 85 DAT, leaf area index (0.70) at 60 DAT and chlorophyll content (41.56) at 55 DAT, respectively. Again, from the interaction, it was observed that all the growth parameters were significantly influenced and increased with suitable variety along with proper nutrient management. Therefore, from this study, it can be decided that BRRI dhan63 fertilized along with 25% less than recommended dose of chemical fertilizer + vermicompost 5 t ha-1 might be the best possible combination for proper growth of plant