Control of Intracellular Francisella tularensis

Reactive nitrogen is critical for the clearance of Francisella tularensis infections. Here we assess the role of nitric oxide in control of intracellular infections in two murine macrophage cell lines of different provenance: the alveolar macrophage cell line, MH-S, and the widely used peritoneal macrophage cell line, J774A.1. Cells were infected with the highly virulent Schu S4 strain or with the avirulent live vaccine strain (LVS) with and without stimuli. Compared to MH-S cells, J774A.1 cells were unresponsive to stimulation and were able to control the intracellular replication of LVS bacteria, but not of Schu S4. In MH-S cells, Schu S4 demonstrated control over cellular NO production. Despite this, MH-S cells stimulated with LPS or LPS and IFN-γ were able to control intracellular Schu S4 numbers. However, only stimulation with LPS induced significant cellular NO production. Combined stimulation with LPS and IFN-γ produced a significant reduction in intracellular bacteria that occurred whether high levels of NO were produced or not, indicating that NO secretion is not the only defensive cellular mechanism operating in virulent Francisella infections. Understanding how F. tularensis interacts with host macrophages will help in the rational design of new and effective therapies

Morphologic Blooming in Breast MRI as a Characterization of Margin for Discriminating Benign from Malignant Lesions

Develop a fully automated, objective method for evaluating morphology on breast MR and evaluate effectiveness of the new morphological method for detecting breast cancers

Breast MRI Interpretation: Text and Online Case Analysis for Screening and Diagnosis.

State-of-the-art resource details effective breast MRI techniques for improved screening and diagnosis Magnetic resonance imaging (MRI) of the breast has evolved into an important breast cancer screening tool and major advance in women's health. Breast MRI is currently the most sensitive detection technique for both non-invasive and invasive cancers and follow-up in women with a new breast cancer diagnosis. It is increasingly becoming the go-to imaging method for screening women at high and intermediate risk of breast cancer and those with dense breast tissue on mammography. Yet despite its re.Breast MRI Interpretation: Text and Online Case Analysis for Screening and Diagnosis -- MedOne Access Information -- Title Page -- Copyright -- Dedication -- Contents -- Foreword -- Preface -- Acknowledgments -- Contributors -- 1 Breast MRI: Overview -- 2 Screening MRI: Who Should Be Screened? -- 3 Screening MRI: DCE-MRI Methods -- 4 Screening MRI: Clinical Implementation -- 5 Diagnostic MRI: Imaging Protocols and Technical Considerations -- 6 Diagnostic MRI Interpretation -- 7 Image Interpretation: Noninvasive Cancer -- 8 Image Interpretation: Invasive Cancer9 Management of Findings Initially Detected at MRI -- 10 Diagnostic MRI: Breast Cancer Applications -- 11 Advanced Breast MRI Techniques -- 12 Semi-Quantitative and Quantitative Analysis of Breast DCE-MRI -- 13 Future Applications: Radiomics and Deep Learning on Breast MRI -- Index -- Additional MedOne Access Information1 online resource (354 pages

Control of Intracellular Francisella tularensis by Different Cell Types and the Role of Nitric Oxide

Reactive nitrogen is critical for the clearance of Francisella tularensis infections. Here we assess the role of nitric oxide in control of intracellular infections in two murine macrophage cell lines of different provenance: the alveolar macrophage cell line, MH-S, and the widely used peritoneal macrophage cell line, J774A.1. Cells were infected with the highly virulent Schu S4 strain or with the avirulent live vaccine strain (LVS) with and without stimuli. Compared to MH-S cells, J774A.1 cells were unresponsive to stimulation and were able to control the intracellular replication of LVS bacteria, but not of Schu S4. In MH-S cells, Schu S4 demonstrated control over cellular NO production. Despite this, MH-S cells stimulated with LPS or LPS and IFN-γ were able to control intracellular Schu S4 numbers. However, only stimulation with LPS induced significant cellular NO production. Combined stimulation with LPS and IFN-γ produced a significant reduction in intracellular bacteria that occurred whether high levels of NO were produced or not, indicating that NO secretion is not the only defensive cellular mechanism operating in virulent Francisella infections. Understanding how F. tularensis interacts with host macrophages will help in the rational design of new and effective therapies

Control of Intracellular Francisella tularensis by Different Cell Types and the Role of Nitric Oxide

Reactive nitrogen is critical for the clearance of Francisella tularensis infections. Here we assess the role of nitric oxide in control of intracellular infections in two murine macrophage cell lines of different provenance: the alveolar macrophage cell line, MH-S, and the widely used peritoneal macrophage cell line, J774A.1. Cells were infected with the highly virulent Schu S4 strain or with the avirulent live vaccine strain (LVS) with and without stimuli. Compared to MH-S cells, J774A.1 cells were unresponsive to stimulation and were able to control the intracellular replication of LVS bacteria, but not of Schu S4. In MH-S cells, Schu S4 demonstrated control over cellular NO production. Despite this, MH-S cells stimulated with LPS or LPS and IFN-were able to control intracellular Schu S4 numbers. However, only stimulation with LPS induced significant cellular NO production. Combined stimulation with LPS and IFN-produced a significant reduction in intracellular bacteria that occurred whether high levels of NO were produced or not, indicating that NO secretion is not the only defensive cellular mechanism operating in virulent Francisella infections. Understanding how F. tularensis interacts with host macrophages will help in the rational design of new and effective therapies