Doubled haploid ramets via embryogenesis of haploid tissue cultures

Tissue culture in the oil palm business is generally concerned with the multiplication (clonal production) of dura, pisifera and tenera palms. These are all normal diploids (2n=2x=36). Sumatra Bioscience has pioneered haploid tissue culture of oil palm (n=x=18). Haploid oil palm is the first step in producing doubled haploid palms which in turn provide parental lines for F1 hybrid production. Chromosome doubling is known to occur during embryogenesis in other haploid cultures, e.g. barley anther culture. Haploid tissue cultures in oil palm were therefore set up to investigate and exploit spontaneous chromosome doubling during embryogenesis. Flow cytometry of embryogenic tissue showed the presence of both haploid (n) and doubled haploid (2n) cells indicating spontaneous doubling. Completely doubled haploid ramets were regenerated suggesting that doubling occurred during the first mitoses of embryogenesis. This is the first report of doubled haploid production in oil palm via haploid tissue culture. The method provides a means of producing a range of doubled haploids in oil palm from the 1,000 plus haploids available at Sumatra Bioscience, in addition the method also produced doubled haploid (and haploid) clones. 1

Direct measurements of dust attenuation in z~1.5 star-forming galaxies from 3D-HST: Implications for dust geometry and star formation rates

The nature of dust in distant galaxies is not well understood, and until recently few direct dust measurements have been possible. We investigate dust in distant star-forming galaxies using near-infrared grism spectra of the 3D-HST survey combined with archival multi-wavelength photometry. These data allow us to make a direct comparison between dust around star-forming regions ($A_{V,\mathrm{HII}}$) and the integrated dust content ($A_{V,\mathrm{star}}$). We select a sample of 163 galaxies between $1.36\le{}z\le1.5$ with H$\alpha$ signal-to-noise ratio $\ge5$ and measure Balmer decrements from stacked spectra to calculate $A_{V,\mathrm{HII}}$. First, we stack spectra in bins of $A_{V,\mathrm{star}}$, and find that $A_{V,\mathrm{HII}}=1.86\,A_{V,\mathrm{star}}$, with a significance of $\sigma=1.7$. Our result is consistent with the two-component dust model, in which galaxies contain both diffuse and stellar birth cloud dust. Next, we stack spectra in bins of specific star formation rate ($\log\,\mathrm{SSFR}$), star formation rate ($\log\,\mathrm{SFR}$), and stellar mass ($\log{}M_*$). We find that on average $A_{V,\mathrm{HII}}$ increases with SFR and mass, but decreases with increasing SSFR. Interestingly, the data hint that the amount of extra attenuation decreases with increasing SSFR. This trend is expected from the two-component model, as the extra attenuation will increase once older stars outside the star-forming regions become more dominant in the galaxy spectrum. Finally, using Balmer decrements we derive dust-corrected H$\alpha$ SFRs, and find that stellar population modeling produces incorrect SFRs if rapidly declining star formation histories are included in the explored parameter space.Comment: Accepted for publication in the Astrophysical Journal (13 pages, 9 figures

Dispersion Coefficients by a Field-Theoretic Renormalization of Fluid Mechanics

We consider subtle correlations in the scattering of fluid by randomly placed obstacles, which have been suggested to lead to a diverging dispersion coefficient at long times for high Peclet numbers, in contrast to finite mean-field predictions. We develop a new master equation description of the fluid mechanics that incorporates the physically relevant fluctuations, and we treat those fluctuations by a renormalization group procedure. We find a finite dispersion coefficient at low volume fraction of disorder and high Peclet numbers.Comment: 4 pages, 1 figure; to appear in Phys. Rev. Let

Exploring the chemical link between local ellipticals and their high-redshift progenitors

We present Keck/MOSFIRE K-band spectroscopy of the first mass-selected sample of galaxies at $z\sim2.3$. Targets are selected from the 3D-HST Treasury survey. The six detected galaxies have a mean [NII]$\lambda$6584/H$\alpha$ ratio of $0.27\pm0.01$, with a small standard deviation of 0.05. This mean value is similar to that of UV-selected galaxies of the same mass. The mean gas-phase oxygen abundance inferred from the [NII]/H$\alpha$ ratios depends on the calibration method, and ranges from 12+log(O/H)$_{gas}=8.57$ for the {Pettini} & {Pagel} (2004) calibration to 12+log(O/H)$_{gas}= 8.87$ for the {Maiolino} {et~al.} (2008) calibration. Measurements of the stellar oxygen abundance in nearby quiescent galaxies with the same number density indicate 12+log(O/H)$_{stars}= 8.95$, similar to the gas-phase abundances of the $z\sim2.3$ galaxies if the {Maiolino} {et~al.} (2008) calibration is used. This suggests that these high-redshift star forming galaxies may be progenitors of today's massive early-type galaxies. The main uncertainties are the absolute calibration of the gas-phase oxygen abundance and the incompleteness of the $z\sim2.3$ sample: the galaxies with detected H$\alpha$ tend to be larger and have higher star formation rates than the galaxies without detected H$\alpha$, and we may still be missing the most dust-obscured progenitors.Comment: 7 pages, 5 figures. Accepted for publication in ApJ

Investigation of Vapor-Phase Lubrication in a Gas Turbine Engine,"

ABSTRACT The liquid oil lubrication system of current aircraft jet engines accounts for approximately 10-15% of the total weight of the engine. It has long been a koal of the aircraft gas turbine industry to reduce this weight. Vapor-Phase Lubrication (VPL) is a promising technology to eliminate liquid oil lubrication. The current investigation resulted in the first gas turbine to operate in the absence of conventional liquid lubrication. A phosphate ester, commercially known as DURAD 6208, was chosen for the test. Extensive research at Wright Laboratory demonstrated that this lubricant could reliably lubricate rolling element bearings in the gas turbine engine environment. The Allison T63 engine was selected as the test vehicle because of its small size and bearing configuration. Specifically, VPL was evaluated in the number eight bearing because it is located in a relatively hot environment, in line with the combustor discharge, and it can be isolated from the other bearings and the liquid lubrication system. The bearing was fully instrumented and its performance with standard oil lubrication was documented. Results of this baseline study were used to develop a thermodynamic model to predict the bearing temperature with VPL. The engine was then operated at a ground idle condition with VPL with the lubricant misted into the #8 bearing at 13 ml/hr. The bearing temperature stabilized at 283°C within 10 minutes. Engine operation was continued successfully for a total of one hour. No abnormal wear of the rolling contact surfaces was found when the bearing was later examined. Bearing temperatures after engine shutdown indicated the bearing had reached thermodynamic equilibrium with its surroundings during the test. After shutdown bearing temperatures steadily decreased without the soakback effect seen after shutdown in standard lubricated bearings. In contrast, the oil lubricated bearing ran at a considerably lower operating temperature (83°C) and was significantly heated by its surroundings after engine shutdown. In the baseline tests, the final bearing temperatures never reached that of the operating VPL system. NOMENCLATUR

Helicity Modulus and Fluctuating Type II Superconductors: Elastic Approximation and Numerical Simulations

We develop the helicity modulus as a criterion for superconducting order in the mixed phase of a fluctuating type II superconductor. We show that there is a duality relation between this helicity modulus and the superfluid density of a system of analog 2D bosons. We show that the vortex line lattice exhibits a perfect Meissner effect with respect to a shearing perturbation of the applied magnetic field, and this becomes our creterion for "longitudinal superconductivity" parallel to the applied field. We present arguments based on the 2D boson analogy, as well as the results of numerical simulations, that suggest that longitudinal superconductivity can persist into the vortex line liquid state for systems of finite thickness, comparable to those commonly found in experiments.Comment: 63 pages, 22 postscript figure

Marginal Pinning of Quenched Random Polymers

An elastic string embedded in 3D space and subject to a short-range correlated random potential exhibits marginal pinning at high temperatures, with the pinning length $L_c(T)$ becoming exponentially sensitive to temperature. Using a functional renormalization group (FRG) approach we find $L_c(T) \propto \exp[(32/\pi)(T/T_{\rm dp})^3]$, with $T_{\rm dp}$ the depinning temperature. A slow decay of disorder correlations as it appears in the problem of flux line pinning in superconductors modifies this result, $\ln L_c(T)\propto T^{3/2}$.Comment: 4 pages, RevTeX, 1 figure inserte

Non-Hermitian Localization and Population Biology

The time evolution of spatial fluctuations in inhomogeneous d-dimensional biological systems is analyzed. A single species continuous growth model, in which the population disperses via diffusion and convection is considered. Time-independent environmental heterogeneities, such as a random distribution of nutrients or sunlight are modeled by quenched disorder in the growth rate. Linearization of this model of population dynamics shows that the fastest growing localized state dominates in a time proportional to a power of the logarithm of the system size. Using an analogy with a Schrodinger equation subject to a constant imaginary vector potential, we propose a delocalization transition for the steady state of the nonlinear problem at a critical convection threshold separating localized and extended states. In the limit of high convection velocity, the linearized growth problem in $d$ dimensions exhibits singular scaling behavior described by a (d-1)-dimensional generalization of the noisy Burgers' equation, with universal singularities in the density of states associated with disorder averaged eigenvalues near the band edge in the complex plane. The Burgers mapping leads to unusual transverse spreading of convecting delocalized populations.Comment: 22 pages, 11 figure

Vortices on Higher Genus Surfaces

We consider the topological interactions of vortices on general surfaces. If the genus of the surface is greater than zero, the handles can carry magnetic flux. The classical state of the vortices and the handles can be described by a mapping from the fundamental group to the unbroken gauge group. The allowed configurations must satisfy a relation induced by the fundamental group. Upon quantization, the handles can carry ``Cheshire charge.'' The motion of the vortices can be described by the braid group of the surface. How the motion of the vortices affects the state is analyzed in detail.Comment: 28 pages with 10 figures; uses phyzzx and psfig; Caltech preprint CALT-68-187

The Home-Based Older People's Exercise (HOPE) trial: study protocol for a randomised controlled trial

<p>Abstract</p> <p>Background</p> <p>Frailty is common in older age, and is associated with important adverse health outcomes including increased risk of disability and admission to hospital or long-term care. Exercise interventions for frail older people have the potential to reduce the risk of these adverse outcomes by increasing muscle strength and improving mobility.</p> <p>Methods/Design</p> <p>The Home-Based Older People's Exercise (HOPE) trial is a two arm, assessor blind pilot randomised controlled trial (RCT) to assess the effectiveness of a 12 week exercise intervention (the HOPE programme) designed to improve the mobility and functional abilities of frail older people living at home, compared with usual care. The primary outcome is the timed-up-and-go test (TUGT), measured at baseline and 14 weeks post-randomisation. Secondary outcomes include the Barthel Index of activities of daily living (ADL), EuroQol Group 5-Dimension Self-Report Questionnaire (EQ-5D) quality of life measure and the geriatric depression scale (GDS), measured at baseline and 14 weeks post-randomisation. We will record baseline frailty using the Edmonton Frail Scale (EFS), record falls and document muscle/joint pain. We will test the feasibility of collection of data to identify therapy resources required for delivery of the intervention.</p> <p>Discussion</p> <p>The HOPE trial will explore and evaluate a home-based exercise intervention for frail older people. Although previous RCTs have used operationalised, non-validated methods of measuring frailty, the HOPE trial is, to our knowledge, the first RCT of an exercise intervention for frail older people that includes a validated method of frailty assessment at baseline.</p> <p>Trial registration</p> <p>ISRCTN: <a href="http://www.controlled-trials.com/ISRCTN57066881">ISRCTN57066881</a></p