Development and Utilization of an Isolation Box Test to Characterize Personality Traits of Dairy Calves

The use of an isolation box test (IBT) to characterize personality traits has been used in non-bovine species with success. We aimed to develop an IBT for dairy calves and determine if the behavioral responses to an IBT were associated with personality traits found from traditional tests (novel person, novel object, and a startle tests) and average daily gain (ADG; Kg/d) through weaning. Calf movement while in the IBT was measured via accelerometers attached to 5 locations on the exterior of the box. A total movement index (TMI) was calculated based on accelerometer readings during the IBT. We performed a principal component analysis on the traditional tests and identified 3 influential factors that we labeled as “fearful,” “bold,” and “active.” Calves were weighed biweekly to track liveweight ADG. Factor scores and ADG were regressed against TMI. A significant negative association was found between the TMI and the factor “active,” indicating the validity of IBT as a tool for assessing some personality traits of dairy calves. Furthermore, TMI had a positive association with ADG through the entire experimental period and thus has potential to help predict performance through weaning. IBT has potential to be used as a personality test in research scenarios. Further development is needed to produce an IBT that would be appropriate to measure animals\u27 responses reliably in production settings. An automated test that can accommodate a wide range of ages and developing a computer learning model to interpret output from the IBT would be a possible option to do so

Duration of androgen deprivation therapy with postoperative radiotherapy for prostate cancer: a comparison of long-course versus short-course androgen deprivation therapy in the RADICALS-HD randomised trial

Background Previous evidence supports androgen deprivation therapy (ADT) with primary radiotherapy as initial treatment for intermediate-risk and high-risk localised prostate cancer. However, the use and optimal duration of ADT with postoperative radiotherapy after radical prostatectomy remains uncertain. Methods RADICALS-HD was a randomised controlled trial of ADT duration within the RADICALS protocol. Here, we report on the comparison of short-course versus long-course ADT. Key eligibility criteria were indication for radiotherapy after previous radical prostatectomy for prostate cancer, prostate-specific antigen less than 5 ng/mL, absence of metastatic disease, and written consent. Participants were randomly assigned (1:1) to add 6 months of ADT (short-course ADT) or 24 months of ADT (long-course ADT) to radiotherapy, using subcutaneous gonadotrophin-releasing hormone analogue (monthly in the short-course ADT group and 3-monthly in the long-course ADT group), daily oral bicalutamide monotherapy 150 mg, or monthly subcutaneous degarelix. Randomisation was done centrally through minimisation with a random element, stratified by Gleason score, positive margins, radiotherapy timing, planned radiotherapy schedule, and planned type of ADT, in a computerised system. The allocated treatment was not masked. The primary outcome measure was metastasis-free survival, defined as metastasis arising from prostate cancer or death from any cause. The comparison had more than 80% power with two-sided α of 5% to detect an absolute increase in 10-year metastasis-free survival from 75% to 81% (hazard ratio [HR] 0·72). Standard time-to-event analyses were used. Analyses followed intention-to-treat principle. The trial is registered with the ISRCTN registry, ISRCTN40814031, and ClinicalTrials.gov , NCT00541047 . Findings Between Jan 30, 2008, and July 7, 2015, 1523 patients (median age 65 years, IQR 60–69) were randomly assigned to receive short-course ADT (n=761) or long-course ADT (n=762) in addition to postoperative radiotherapy at 138 centres in Canada, Denmark, Ireland, and the UK. With a median follow-up of 8·9 years (7·0–10·0), 313 metastasis-free survival events were reported overall (174 in the short-course ADT group and 139 in the long-course ADT group; HR 0·773 [95% CI 0·612–0·975]; p=0·029). 10-year metastasis-free survival was 71·9% (95% CI 67·6–75·7) in the short-course ADT group and 78·1% (74·2–81·5) in the long-course ADT group. Toxicity of grade 3 or higher was reported for 105 (14%) of 753 participants in the short-course ADT group and 142 (19%) of 757 participants in the long-course ADT group (p=0·025), with no treatment-related deaths. Interpretation Compared with adding 6 months of ADT, adding 24 months of ADT improved metastasis-free survival in people receiving postoperative radiotherapy. For individuals who can accept the additional duration of adverse effects, long-course ADT should be offered with postoperative radiotherapy. Funding Cancer Research UK, UK Research and Innovation (formerly Medical Research Council), and Canadian Cancer Society

Adding 6 months of androgen deprivation therapy to postoperative radiotherapy for prostate cancer: a comparison of short-course versus no androgen deprivation therapy in the RADICALS-HD randomised controlled trial

Background Previous evidence indicates that adjuvant, short-course androgen deprivation therapy (ADT) improves metastasis-free survival when given with primary radiotherapy for intermediate-risk and high-risk localised prostate cancer. However, the value of ADT with postoperative radiotherapy after radical prostatectomy is unclear. Methods RADICALS-HD was an international randomised controlled trial to test the efficacy of ADT used in combination with postoperative radiotherapy for prostate cancer. Key eligibility criteria were indication for radiotherapy after radical prostatectomy for prostate cancer, prostate-specific antigen less than 5 ng/mL, absence of metastatic disease, and written consent. Participants were randomly assigned (1:1) to radiotherapy alone (no ADT) or radiotherapy with 6 months of ADT (short-course ADT), using monthly subcutaneous gonadotropin-releasing hormone analogue injections, daily oral bicalutamide monotherapy 150 mg, or monthly subcutaneous degarelix. Randomisation was done centrally through minimisation with a random element, stratified by Gleason score, positive margins, radiotherapy timing, planned radiotherapy schedule, and planned type of ADT, in a computerised system. The allocated treatment was not masked. The primary outcome measure was metastasis-free survival, defined as distant metastasis arising from prostate cancer or death from any cause. Standard survival analysis methods were used, accounting for randomisation stratification factors. The trial had 80% power with two-sided α of 5% to detect an absolute increase in 10-year metastasis-free survival from 80% to 86% (hazard ratio [HR] 0·67). Analyses followed the intention-to-treat principle. The trial is registered with the ISRCTN registry, ISRCTN40814031, and ClinicalTrials.gov, NCT00541047. Findings Between Nov 22, 2007, and June 29, 2015, 1480 patients (median age 66 years [IQR 61–69]) were randomly assigned to receive no ADT (n=737) or short-course ADT (n=743) in addition to postoperative radiotherapy at 121 centres in Canada, Denmark, Ireland, and the UK. With a median follow-up of 9·0 years (IQR 7·1–10·1), metastasis-free survival events were reported for 268 participants (142 in the no ADT group and 126 in the short-course ADT group; HR 0·886 [95% CI 0·688–1·140], p=0·35). 10-year metastasis-free survival was 79·2% (95% CI 75·4–82·5) in the no ADT group and 80·4% (76·6–83·6) in the short-course ADT group. Toxicity of grade 3 or higher was reported for 121 (17%) of 737 participants in the no ADT group and 100 (14%) of 743 in the short-course ADT group (p=0·15), with no treatment-related deaths. Interpretation Metastatic disease is uncommon following postoperative bed radiotherapy after radical prostatectomy. Adding 6 months of ADT to this radiotherapy did not improve metastasis-free survival compared with no ADT. These findings do not support the use of short-course ADT with postoperative radiotherapy in this patient population

Do Walking Distance and Time Away from the Paddock Influence Daily Behaviour Patterns and Milk Yield of Grazing Dairy Cows?

In pasture-based systems, cows may spend several hours away from the paddock and may also walk several kilometres to meet daily milking requirements; this could lead cows to experience time constraints for grazing, ruminating and lying time in the paddock. This study investigated how walking distance and time spent away from the paddock affected daily behavioural patterns (i.e., grazing, ruminating and lying time) and milk yield. Dairy cows were managed in three rotationally grazed groups (n = 29 cows each) on the same farm and milked twice daily. A triaxial ear tag accelerometer on each cow recorded daily duration of grazing and ruminating, and a leg-based accelerometer recorded the daily lying time, for 13 days. GPS collars on four cows per group recorded the daily walking distance and total time away from the paddock for the group. A mixed repeated measures model tested how time off-paddock and walking distance affected the daily behavioural patterns; age, breed, milk yield and maximum ambient temperature were used as covariates with group as the observational unit. A second similar model tested how these factors affected milk yield. Walking distance and time spent away from the paddock were not correlated. When daily walking distance increased (to a maximum of 4 km/d), cows spent more time grazing and less time ruminating, but lying time was not affected. This result may, in part, be related to the greater energy expenditure demands for walking longer distances and milk production. When time away from the paddock increased (to a maximum of 4 h/d), cows spent less time lying, but grazing and ruminating times were not affected. Milk yield was not affected by walking distance, but one of the groups experienced a lower milk yield when time away from the paddock was increased. This result suggests that, for some cows, lying times may be shorter when experiencing a longer time away from the paddock, which may also affect milk yield. Overall, this study indicates that paddock behaviours are associated with walking distance to the milking parlour and time spent away from the paddock. Efforts to reduce walking distance and time spent away from the paddock are likely to provide cows with greater opportunity to engage in daily behaviours in the paddock that meet their needs and maintain their milk yield

Anticipatory behaviour as an indicator of the welfare of dairy calves in different housing environments.

Anticipatory behaviour occurs in the period before a reward or other positive event is presented and has been interpreted as an indicator of the welfare and emotional state of animals. The use of this indicator has received limited attention in dairy calves. Therefore, we investigated how anticipatory behaviour is affected by housing environment and reward quality, and if anticipatory behaviour changes when reward quality changes unexpectedly. Sixteen pairs of calves were assigned to treatments in a 2 x 2 factorial design (two housing environment and two reward quality combinations). Housing was either basic (2 m2/calf, river stone surface) or enriched (5 m2/calf, woodchip, and enrichment items), and the reward was access to either an additional basic or enriched pen. Calves were conditioned to anticipate reward pen access; anticipatory behaviour toward receiving the reward pen was measured. Signaling reward access increased the frequency of transitions between behaviours and duration of touching and looking at the signal and exit door. Basic-housed calves showed more anticipatory behaviour (increased frequency of transitions between behaviours) and decreased latency to access the reward compared to enriched-housed calves, but the reward pen quality had no effect on anticipatory behaviour. When the reward pen quality changed from enriched to basic unexpectedly, resulting in sudden reward loss, basic-housed calves decreased, while enriched-housed calves increased, anticipatory behaviour. However, there was no change in anticipatory behaviour during reward gain (change from basic to enriched reward pen). Our findings align with previous work showing that animals in basic housing show more anticipation for a reward, and demonstrate suppressed behavioural response when experiencing reward loss, suggesting greater sensitivity to reward. Sensitivity to reward has associations with mood state; thus, calves in basic environments may experience a more negative emotional state, and thus reduced welfare, compared to calves in enriched environments

Calves Use an Automated Brush and a Hanging Rope When Pair-Housed

Calf housing often only meets the basic needs of calves, but there is a growing interest in providing enrichments. This study described the behaviour of calves when they were given the opportunity to interact with two commonly available enrichment items. Female and male calves (approximately 11 days old) were pair-housed in 8 identical pens fitted with an automated brush and a hanging rope. Frequency and duration of behaviours were recorded on 3 separate days (from 12:00 until 08:00 the following day. Calves spent equal time using the brush and rope (27.1 min/day), but there was less variation in the use of the brush as opposed to the rope (coefficient of variation, CV: 23 vs. 78%, respectively). Calves had more frequent (94 bouts, CV: 24%) and shorter (17.8 s/bout, CV: 24%) brush use bouts compared to fewer (38 bouts, CV: 43%) and longer (38.3 s/bout, CV: 53%) rope use bouts. There was a diurnal pattern of use for both items. Frequency of play was similar to rope use, but total time playing was 8% of rope and brush use. Variability among calves suggested that individual preference existed; however, the social dynamics of the pair-housed environment were not measured and therefore could have influenced brush and rope use. Multiple enrichment items should be considered when designing improvements to calf housing.Land and Food Systems, Faculty ofNon UBCReviewedFacult

Behavioral responses of dairy cows and their calves to gradual or abrupt weaning and separation when managed in full- or part-time cow-calf contact systems

ABSTRACT: There is growing interest in managing cow and calf together for a prolonged period, but concerns remain about how best to wean and separate the cow and calf to minimize stress. One approach is to progressively reduce suckling opportunity over time, as in nature. There is also interest in part-time contact (suckling for part of the day) to improve milk yield for the farmer and potentially reduce stress at weaning and separation. The primary objective of this study was to compare the behavioral responses of dairy cows to gradual or abrupt weaning and separation, when managed either full or part time with their calves; a secondary focus was the vocal responses of calves under these management conditions. In a 3 × 2 factorial design (n = 14/ treatment), dairy cows and their calves (n = 84 in 7 blocks of 12 cow-calf pairs) were assigned to one of 3 dam-contact treatments at birth: (1) full-time contact between the dam and calf, apart from milking times (total 23 h/d) (2) part-time contact between the dam and calf, between morning and afternoon milking only (total 10 h/d); (3) no contact, where the dam and calf were separated after leaving the maternity pen and had no further contact. At wk 8, one of 2 weaning treatments was assigned: (1) gradual weaning by reduced contact time (50%, then 25% of original dam-contact time in wk 8 and 9, respectively), or gradually reduced milk allowance for no-contact calves (50%, then 25% of estimated 12-L milk intake in wk 8 and 9, respectively) until complete milk removal and dam-calf separation at wk 10; (2) abrupt weaning where milk removal occurred simultaneously with dam-calf separation at wk 10, or only milk removal for no-contact calves. Overall, part-time contact did not reduce weaning and separation distress for cows or calves for either weaning method. Part-time cows showed reduced behavioral responses to separation (greater lying time and less searching behavior), especially on the day of and 24 h after separation, but they showed a similarly strong vocal response to separation as full-time cows. Part-time calves made substantially more high-pitched vocalizations than full-time calves at 24 h after separation. Furthermore, gradual weaning by reducing contact time did not seem to better prepare cows or calves for complete milk removal and separation; most behavioral and vocal responses occurred on the day of separation for gradual-weaning cows and calves, but 24 h later, the reverse occurred for abrupt-weaning cows and there was no difference between gradual- and abrupt-weaning calves. Our results suggest that part-time contact and gradual-weaning conditions likely resulted in hunger and expectation for reunion, which together may have exaggerated behavioral responses at separation. Temporary daily separations may not have increased independence of cow and calf, and the gradual-weaning method here may not have reduced milk intake by calves. These elements are important criteria to facilitate the weaning process, so future work should explore ways to gradually reduce milk intake and promote social independence of cow and calf to minimize weaning distress

Dairy cows’ motivation to nurse their calves

Abstract When weaning offspring, female mammals limit nursing opportunities. This study aimed to investigate whether imposing a gradual reduction in daily contact time, by separating cows from their calves as an attempt to stimulate weaning, reduced dairy cows’ motivation to nurse their calves. For seven weeks, 84 Holstein–Friesian cow-calf pairs were housed with either full-time (23 h contact/d), part-time (10 h contact/d), or no contact. In the following two weeks, half of full- and part-time pairs were subjected to reduced contact (50% of initial contact in week 8, 25% of initial contact in week 9), while the other half continued with unchanged contact. In weeks 8 and 9, cows’ motivation to obtain full contact to and opportunity to nurse their calves was measured using weighted push gates using a novel maximum price paid method providing an alternative choice to the cows to reduce frustration. Cows with reduced calf contact were more motivated than cows with unchanged contact; however, cows used the alternative choice less than expected. The results show that cows’ motivation for full calf contact and opportunity to nurse increases when daily calf contact is reduced, illustrating that dairy cows are motivated to continue nursing their 9- to 10-week-old calves

Calf approach responses in the cognitive bias task

Calf approach responses to each screen for each session before and after disbudding (%). Approach responses have been averaged across trials (23 each of positive and negative screens; 5 of each ambigous screen) within each sessio

Data from: Pain and pessimism: dairy calves exhibit negative judgement bias following hot-iron disbudding

Pain is defined as an unpleasant sensory and emotional experience associated with actual or potential tissue damage, but emotional states are difficult to directly assess in animals. Researchers have assessed pain using behavioural and physiological measures, but these approaches are limited to understanding the arousal rather than valence of the emotional experience. Cognitive bias tasks show that depressed humans judge ambiguous events negatively and this technique has been applied to assess emotional states in animals. However, limited research has examined how pain states affect cognitive processes in animals. Here we present the first evidence of cognitive bias in response to pain in any non-human species. In two experiments, dairy calves (n = 17) were trained to respond differentially to red and white video screens and then tested with unreinforced ambiguous colours in two or three test sessions before and two sessions after the routine practice of hot-iron disbudding. After disbudding calves were more likely to judge ambiguous colours as negative. This ‘pessimistic’ bias indicates that post-operative pain following hot-iron disbudding results in a negative change in emotional state