Anti-inflammatory activity of seven plant species with potential use as livestock feed additives

Please read abstract in the article.The National Research Council (NRF) South Africa, Onderstepoort Veterinary Institute (OVI), the University of Pretoria and Red Meat Research and Development South Africa.http://www.elsevier.com/locate/sajbhj2024ChemistryParaclinical SciencesSDG-03:Good heatlh and well-beingSDG-15:Life on lan

Optimizing extraction of pelargonium sidoides roots : impact of ethanol concentration on biological activity of extracts

Pelargonium sidoides DC. (Geraniaceae) is an important indigenous medicinal plant in South Africa, historically employed by various ethnic groups to treat respiratory and gastrointestinal ailments. The proprietary herbal tincture, Umckaloabo , utilizes an ethanolic extract (EPs 7630) from the roots of P. sidoides, and has demonstrated effectiveness in alleviating symptoms of respiratory infections. P. sidoides roots contain numerous highly oxygenated coumarins and phenolic metabolites, notably the marker compound umckalin. While Umckaloabo is prepared using 11 % ethanol extraction, the South African commercial market predominantly employs 60 % ethanol extraction due to its consistent umckalin yield and antimicrobial activity. The effects of these extraction methods on chemical composition and in vitro pharmacological activity remain poorly understood. This study confirmed superior antibacterial and antifungal activity in the 60 % ethanol extracts prepared from six root samples compared to the 11 % ethanol extracts. MIC values for both Gram-negative and Gram-positive bacteria ranged from 0.078 to 2.5 mg/mL in the 60 % extracts. Remarkably, two of the root extracts exhibited excellent to very good activity against C. albicans and C. neoformans with MIC values of 0.039 and 0.078 mg/mL, respectively. A positive correlation was identified between total minimum inhibitory concentration (MIC) levels and polyphenol content, rather than umckalin levels. The influence of these extracts on the pro-inflammatory cytokine IL-6 was assessed. Both 60 % and 11 % root extracts of P. sidoides at 100 mg/mL significantly reduced IL-6 production, with the 60 % extracts demonstrating a more pronounced effect (p = 0.008). Statistical analysis revealed significant differences between 11 % and 60 % ethanol extractions in terms of average MIC overall, average Gram-negative MIC, average Gram-positive MIC, fungal MIC, and IL-6 levels. Extracts with higher polyphenol values exhibited superior antimicrobial activity and antioxidant potential, suggesting that polyphenol content may serve as a more reliable indicator of antimicrobial activity than umckalin levels. Additionally, polyphenol levels in the roots may vary with altitude and other environmental factors, warranting further research.The National Research Foundation and Nativa (Pty) Ltd, South Africa.http://www.elsevier.com/locate/sajbam2024ChemistryParaclinical SciencesNon