Effect of Date Palm Pollen Suspension on Ovarian Function and Fertility in Adult Female Rats Exposed to Lead Acetate

       This study present the effects of Date Palm Pollen grains (DPP) (phoenix dactylifera) on ovary function and fertility in adult  female rats exposed to lead acetate. Forty adult female albino rats were divided randomly into four equal groups. The first control  group was given orally (1 ml) distal water,(T1) group given orally 150 mg / kg .B.W. DPP ( 0.5ml),(T2) group given orally  (10) mg / kg. B.W. lead acetate (1 ml) ,(T3) group given daily by oral administration of both DPP150 mg/kg B.W. and 10 mg / kg.  B.W. lead acetate .all animals treated via gavages needle for 6 weeks.At the end of experiment blood were collected to determine serum LH & FSH  level .      Treated( T1)  group showed the increase levels of LH  were on significant importance at a level of < 0.05 in comparison with zero time of the same group and in comparison with control group . To contrast to treated(T1)  group, the level on LH showed a significantly decrease levels in animals on treated (T2) group exposure lead acetate at a dose rate of  10 mg / kg. B.W daily .  The significant decrease in LH were showed in comparison the value with that at zero day of the same group and at a time on experiment 14, 28, 42 day post exposure in comparison with control group and with (T1) group exposed to DPP. Animal on  treated (T3) group exposed of both DPP at 150 mg /kg. B. W. and lead acetate 10 mg / kg. B.W. for 6 weeks didn`t show any significant changes during the experiment.      Animals on( T1) group that exposed to DPP at a dose rate on 150 mg / k. B.W. for 6 weeks showed a significant increase FSH level in serum of exposed animal in comparison with zero time of the same group and in comparison with contrl group  . While those on (T2) group which exposed to lead acetate at a dose rate of 10 mg / kg. B.W. for 6 weeks showed a significant decrease in FSH level in serum on day 14 and 42 following the exposure the decrease in FSH level in serum may significant in comparison with zero day of same group and with level on T1 group and control group. Animal on group T3 didn`t show any significant changes during the experiment.            This study conclude that DPP has obvious improvement effect on fertility hormones of Adult  female rats exposed to lead acetate

The Clinical and Hematological changes in rabbits exposed to leaves of Urtica dioica under experimental conditions

     The study  was conducted on 20 female local breed rabbits , of 1-2 years age , of 1- 2 kg body weight . In first part of the study  , 10 animals were randomly  divided into two groups of 5 each. The first group was exposed to leaves of Urtica dioica  in green phase at a dose rate of 50 grams / kg b.w daily for 10 days  , while those of the second group left without exposure as a control group . In the second part of the study ,  animals of the first group exposed to leaves in dry phase , at a dose rate of 5 g/kg b.w. daily for three weeks, as a powder mixed with the concentrated food.  While those of the second group left as a control group without exposure. The  main dependent parameters in the study  were ,clinical parameters ( body temperature , heart beat , respiratory rate, body weight , in addition to monitor  any abnormal signs appear  on the animals) . The  main hematological  parameters which depended in the study were included , RBC count , WBC count , Hb concentration   , PCV percentage  , Red cell indices , Bleeding time and Clotting time  .     The results of the study revealed the following : In first part  clinically  ;  some rabbits showed  strong heart beat , in other heart beat decreased ; respiratory  rate  decreased with noisy vesicular murmurs were heard  . Body weight and body temperature were decreased. In the fourth day post exposure to plant one rabbit  showed softy feces . In the 16th day post exposure one animal died ,  followed  by another on in 18th day of the experiment  . The post mortem findings were pale mucous membranes  , stomach impacted with food with ulceration of mucus membranes . severe congestion of liver and gastrointestinal tract . sever swelling of kidney , urinary bladder filled with  urine of dark yellow color .Hematologically clotting time prolonged , with decrease PCV , and White blood cells counts ; while  Hb concentration did not showed any changes      In second part: Heart beats , respiratory rates and body temperature  were increased , while body weight decreased . Prolongation of clotting and bleeding  time .The hematological pictures revealed that , Hemoglobin level and PCV values  were decreased .The total erythrocyte count did not showed any significant changes during the study . The mean corpuscular volume and mean corpuscular hemoglobin ,the mean corpuscular hemoglobin concentration were significantly decreased .The total leucocytes count ,the percentage of heterophils, and  monocytes percentage were increase.  The lymphocytic and, esinophils percentage were significantly decreased .The basophiles values did not showed any significant changes during the study