5-Carb­oxy-2,4-dihy­droxy­anilinium chloride dihydrate

In the title compound, C7H8NO4 +·Cl−·2H2O, the organic mol­ecule is almost planar with an r.m.s. deviation of 0.0164 Å for all non-H atoms. An S(6) ring motif is formed due to an intra­molecular O—H⋯O hydrogen bond. In the crystal, the mol­ecules are linked into a three-dimensional network by N—H⋯Cl, N—H⋯O, O—H⋯Cl and O—H⋯O hydrogen bonds

5-Carb­oxy-2,4-dihy­droxy­anilinium chloride

In the title salt, C7H8NO4 +·Cl−, the organic group is planar with an r.m.s. deviation of 0.0265 Å. An S(6) ring motif is formed due to an intra­molecular O—H⋯O hydrogen bond. The compound consists of dimers due to inter­molecular O—H⋯O hydrogen bonds with an R 2 2(8) ring motif. The dimers are inter­linked through strong N—H⋯Cl and O—H⋯Cl hydrogen bonds, resulting in a three-dimensional polymeric network

Enhanced biocidal activity of Au nanoparticles synthesized in one pot using 2, 4-dihydroxybenzene carbodithioic acid as a reducing and stabilizing agent

Background The conjugation of gold nanoparticles with biocides such as natural products, oligosaccharides, DNA, proteins has attracted great attention of scientists recently. Gold NPs covered with biologically important molecules showed significant enhancement in biological activity in comparison with the activity of the free biocides. However, these reports are not very systematic and do not allow to draw definitive conclusions. We therefore embarked in a systematic study related to the synthesis and characterization of biocidal activities of Au nanoparticles conjugated to a wide variety of synthetic and natural biomolecules. In this specific report, we investigated the activity of a synthetic biocide, 2-4, Dihydroxybenzene carbodithioic acid (DHT). Results Au nanoparticles (NP) with a mean size of about 20 nm were synthesized and functionalized in one pot with the help of biocide 2,4-Dihydroxybenzene carbodithioic acid (DHT) to reduce HAuCl4 in aqueous solution. Conjugation of DHT with gold was confirmed by FT-IR and the amount of DHT conjugated to the Au nanoparticles was found to be 7% by weight by measuring the concentration of DHT in the supernatant after centrifugation of the Au NPs. To ascertain the potential for in vivo applications, the stability of the suspensions was investigated as a function of pH, temperature and salt concentration. Antibacterial, antifungal, insecticidal and cytotoxic activities of the Au-DHT conjugates were compared with those of pure DHT and of commercially available biocides. In all cases, the biocidal activity of the Au-DHT conjugates was comparable to that of commercial products and of DHT. Conclusions Since the DHT concentration in the Au-DHT conjugates was only about 7%, our results indicate that conjugation to the Au NPs boosts the biocidal activity of DHT by about 14 times. The suspensions were found to be stable for several days at temperatures of up to 100°C, salt concentrations up to 4 mol/L and a pH range of 2-13

Methyl 2,4-dihydroxy-5-(2-methylpropanamido)benzoate

In the title compound, C12H15NO5, the dihedral angle between the benzene ring and the C atoms of the terminal isopropyl group is 83.48 (16)°. Intramolecular N—H...O and O—H...O hydrogen bonds generate S(5) and S(6) rings, respectively. In the crystal, molecules are linked by O—H...O hydrogen bonds, generating C(7) chains propagating in [001]. Weak aromatic π–π stacking [centroid–centroid separation = 3.604 (3) Å] is also observed

Methyl 2,4-dihydroxy-5-(4-nitrobenzamido)benzoate

In the title compound, C15H12N2O7, the dihedral angle between the aromatic rings is 4.58 (13)° and the nitro group is rotated from its attached ring by 18.07 (17)°. Intramolecular N—H...O and O—H...O hydrogen bonds generate S(5) and S(6) rings, respectively. In the crystal, molecules are linked by O—H...O hydrogen bonds, generating [001] C(7) chains. The chains are linked by C—H...O interactions, forming a three-dimensional network, which incorporates R22(7) and R22(10) loops

Fabrication and Characterization of Chitosan–Vitamin C–Lactic Acid Composite Membrane for Potential Skin Tissue Engineering

Recent advances in tissue engineering have potential for the development of improved substitutes for damaged skin tissues. Vitamin C and lactic acid are well-known wound healing accelerators while chitosan is an important biomaterial having wound healing capabilities. However, addition of vitamin C induces fragility to the chitosan–lactic acid membranes. Therefore, the current study was designed to fabricate an intact chitosan–vitamin C–lactic acid composite membrane that may synergize the critical properties of every individual component for potential skin tissue engineering. For this purpose, different concentrations of glycerol and polyethylene glycol (PEG) were added to strengthen the chitosan–vitamin C–lactic acid membranes. The prepared membranes were characterized by Fourier transform infrared spectroscopy, X–ray diffraction, and field emission scanning electron microscopy. Moreover, the biocompatibility of the prepared membranes was evaluated with fibroblast NIH 3T3 cells. The results showed that addition of glycerol and PEG has improved the strength of chitosan–vitamin C–lactic acid composite membrane. Characterization studies revealed the successful synthesis of chitosan–vitamin C–lactic acid composite membrane. Moreover, the prepared membranes showed excellent biocompatibility with NIH 3T3 cells. However, it is important to note that cells showed more attachment and spreading on porous chitosan composites membranes as compared to nonporous membranes. This study provided a base for the development of an intact chitosan–vitamin C–lactic acid composite membrane for skin tissue engineering. However, further preclinical and clinical studies are required for its practical applications in skin tissue engineering

Synthesis of Copper Oxide-Based Nanoformulations of Etoricoxib and Montelukast and Their Evaluation through Analgesic, Anti-Inflammatory, Anti-Pyretic, and Acute Toxicity Activities

Copper oxide nanoparticles (CuO NPs) were synthesized through the coprecipitation method and used as nanocarriers for etoricoxib (selective COX-2 inhibitor drug) and montelukast (leukotriene product inhibitor drug) in combination therapy. The CuO NPs, free drugs, and nanoformulations were investigated through UV/Vis spectroscopy, FTIR spectroscopy, XRD, SEM, and DLS. SEM imaging showed agglomerated nanorods of CuO NPs of about 87 nm size. The CE1, CE2, and CE6 nanoformulations were investigated through DLS, and their particle sizes were 271, 258, and 254 nm, respectively. The nanoformulations were evaluated through in vitro anti-inflammatory activity, in vivo anti-inflammatory activity, in vivo analgesic activity, in vivo anti-pyretic activity, and in vivo acute toxicity activity. In vivo activities were performed on albino mice. BSA denaturation was highly inhibited by CE1, CE2, and CE6 as compared to other nanoformulations in the in vitro anti-inflammatory activity. The in vivo bioactivities showed that low doses (5 mg/kg) of nanoformulations were more potent than high doses (10 and 20 mg/kg) of free drugs in the inhibition of pain, fever, and inflammation. Lastly, CE2 was more potent than that of other nanoformulations