Effects of Mega Dose Micronutrient Supplementation On Serum Zinc, Retinol and Immune Status of Adult Males and Females Diagnosed with and Without HIV, Malaria and TB in Western Kenya – An Unpublished Perspective as at The Year 2004

Background: The role of micronutrients in management of HIV/AIDS, malaria and TB remains poorly understood worldwide. Objectives: To assess differences in mega dose nutritional management between HIV-seronegative and seropositive adult males and females diagnosed with HIV at Voluntary Testing and Counseling Centers (VCT) in Western Kenya. Methods: This was a randomized controlled study in which 90 subjects were recruited on the basis of an HIV-seropositive result from a voluntary and counseling center (VCT) using rapid HIV test kits. They were evaluated at baseline and every 4 weeks for 3 months to establish their clinical, biochemical and immunological status. After 12 weeks, 74 clients were still in the study, 9 were lost to follow-up while 7 had died. Of the 74 who completed the study, confirmation of baseline HIV status by ELIZA revealed that 63 were HIV-seropositive while 11 were HIV-seronegative despite losing spouses to HIV/AIDS. Correlations between parameters at baseline, during and after intervention were determined; Spearman’s Rho Coefficients indicating the level of significance. Group means were used to compare continuous data while categorical data was compared using Chi-Square. Results: Significant reductions in the clinical manifestation of disease were noted in the cohort after intervention for 12 weeks. Despite the large and different micronutrient dosages used between the two study arms, the only difference by arm of intervention was in the serum vitamin E level at 4 weeks which was much higher in arm 1 than it was in arm 2 of the study (p = 0.005). This might have been occasioned by the significant repletion of zinc in both arms, probably because use of citric acid in both arms improved zinc up-take from the supplements, food and/or reserves enabling other nutrients to be appropriately restored in both arms, these supporting the decision to pool the study arms and compare differences by HIV-seronegative and seropositive, notwithstanding the small sample sizes recruited but which nonetheless were our study limitation. Independent of the intervention arms, reduction of viral load by more than 0.5 log10 copies/ml correlated with higher baseline optical densities of HIV antibodies (P = 0.016) and higher baseline viral loads (p = 0.0001). A lower optical density of HIV antibodies at baseline correlated with higher serum zinc levels at 12 weeks (p = 0.008) and a lower Body Mass Index (BMI) at baseline (p = 0.029). Independent of the arm of study, a significant increase in CD4 cells counts post intervention correlated with lower baseline viral loads (p = 0.010), lower baseline NK cell counts (p = 0.007

Use of kitchen steel wool as oxygen absorber improves storage retention of beta-carotene in solar-dried vegetables

Abstract: Vitamin A deficiency remains a major health concern in developing countries whereas the season availability of vegetables could provide for vitamin A. Dehydration is widely used to preserve dark green leafy vegetables (DGLV) but storage in normal atmosphere condition losses beta-carotene by oxidation, therefore requiring use of an oxygen absorber. The study examined use of kitchen steel wool as an oxygen absorber in reducing the loss of beta-carotene content in three indigenous DGLVs that were solar dried and stored for a period of 168 days in four different packing conditions. Fresh vegetables contained between 781.94 to 1047.42 μg/g dry matter (DM) beta-carotene, reducing significantly (p=0.01) to between 653.63 to 712.99 μg/g DM after dehydration. Steel wool oxygen absorber significantly improved (p = 0.02) beta-carotene retention, recording a loss of 19.5 to 37.6% compared to 47 to 72% in normal conditions. Storage of DGLVs under kitchen steel wool oxygen absorber preserves vegetables and retains high levels of beta-carotene

Fabrication of Nanostructured Polyamic Acid Membranes for Antimicrobially Enhanced Water Purification

Water scarcity and quality challenges facing the world can be alleviated by Point-of-Use filtration devices (POU). The use of filtration membranes in POU devices has been limited largely because of membrane fouling, which occurs when suspended solids, microbes, and organic materials are deposited on the surface of filtration membranes significantly decreasing the membrane lifespan, thereby increasing operation costs. There is need therefore to develop filtration membranes that are devoid of these challenges. In this work, nanotechnology was used to fabricate nanostructured polyamic acid (nPAA) membranes, which can be used for microbial decontamination of water. The PAA was used as support and reducing agent to introduce silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) with antimicrobial properties. The nPAA membranes were fabricated via thermal and wet phase inversion technique and then tested against Escherichia coli and Staphylococcus aureus following standard tests. The resulting nanoparticles exhibited excellent dispersibility and stability as indicated by the color change of the solution and increments of optical density at 415 nm for AgNPs and 520 nm for AuNPs. The wet phase inversion process used produced highly porous, strong, and flexible nPAA membranes, which showed well-dispersed spherical AuNPs and AgNPs whose rough average size was found to be 35 nm and 25 nm, respectively. The AgNPs demonstrated inhibition for both gram positive E. coli and gram negative S. aureus, with a better inhibitory activity against S. aureus. A synergistic enhancement of AgNPs antimicrobial activity upon AuNPs addition was demonstrated. The nPAA membranes can thus be used to remove microbials from water and can hence be used in water purification