Inflammatory Stroma of Lymphoepithelioma-like Carcinoma of the Cervix: Immunohistochemical Study of 3 Cases and Review of the Literature

The aim of this study was to characterize the inflammatory infiltrate of the lymphoepithelioma-like carcinoma, a variant of squamous cell carcinoma clinically associated with a good prognosis. Immunohistochemistry was used to characterize 3 cases of lymphoepithelioma-like carcinoma in the uterine cervix, diagnosed over a period of 3 yr. The patients were between 30 and 50 yr old. Their cervical smears had shown atypical squamous cells of unknown significance or high-grade squamous intraepithelial lesions, and the initial cervical biopsy showed high-grade cervical intraepithelial neoplasia (CIN3) or squamous cell carcinoma. All patients underwent a Wertheim operation, 2 of which were preceded by a cervicectomy. Microscopically, all tumors were characterized by poorly defined sheets of undifferentiated squamous cells with a syncytial pattern and a dense background of infiltrating lymphocytes. Immunohistochemical analysis revealed that the lymphocytes were predominantly CD3 + and CD8 + T cells. The tumor lobules contained isolated CD8 + T cells, whereas CD4 + T cells and CD20 + B cells surrounded the tumor lobules. CD56 + NK cells and CD79 + B cells were scattered in the tumor tissue. An in situ hybridization staining for Epstein-Barr encoding region was negative but all cases were immunohistochemically positive for P16. Follow-up varied between 2 mo and 2.7 yr. All 3 patients were disease free. Lymphoepithelioma-like carcinoma of the uterine cervix is a variant of squamous cell carcinoma, known for its better prognosis. The good prognosis of this tumor is potentially explained by the high levels of infiltrating CD8 + T cells.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

A Prospective Study of Lymphocytes Infiltrating the Breast Cancer Microenvironment

Abstract 1105info:eu-repo/semantics/publishe

Characteristics of B cells present in organized tumor-associated tertiary lymphoid structures in human breast cancer

Poster non publiéinfo:eu-repo/semantics/nonPublishe

CXCL13-producing follicular helper T cells in human breast cancer.

info:eu-repo/semantics/publishe

Characterization and organization of infiltrating B-cells in human breast cancer

info:eu-repo/semantics/nonPublishe

Lymphocytes infiltrating breast cancer :density, composition and organization

Abstract 50Pinfo:eu-repo/semantics/publishe

The composition and organization of lymphocytes infiltrating human breast cancer

Poster presentation selected for a poster walkinfo:eu-repo/semantics/publishe

CXCL13-producing extrafollicular Tfh-like CD4+ T cells in human breast cancer

info:eu-repo/semantics/publishe

A simple and rapid protocol to non-enzymatically dissociate fresh human tissues for the analysis of infiltrating lymphocytes.

The ability of malignant cells to evade the immune system, characterized by tumor escape from both innate and adaptive immune responses, isnow accepted as an important hallmark of cancer. Our research on breast cancer focuses on the active role that tumor infiltrating lymphocytesplay in tumor progression and patient outcome. Toward this goal, we developed a methodology for the rapid isolation of intact lymphoid cellsfrom normal and abnormal tissues in an effort to evaluate them proximate to their native state. Homogenates prepared using a mechanicaldissociator show both increased viability and cell recovery while preserving surface receptor expression compared to enzyme-digested tissues.Furthermore, enzymatic digestion of the remaining insoluble material did not recover additional CD45+ cells indicating that quantitative and qualitative measurements in the primary homogenate likely genuinely reflect infiltrating subpopulations in the tissue fragment. The lymphoidcells in these homogenates can be easily characterized using immunological (phenotype, proliferation, etc.) or molecular (DNA, RNA and/orprotein) approaches. CD45+ cells can also be used for subpopulation purification, in vitro expansion or cryopreservation. An additional benefitof this approach is that the primary tissue supernatant from the homogenates can be used to characterize and compare cytokines, chemokines,immunoglobulins and antigens present in normal and malignant tissues. This protocol functions extremely well for human breast tissues andshould be applicable to a wide variety of normal and abnormal tissues.The video component of this article can be found at http://www.jove.com/video/52392/info:eu-repo/semantics/publishe