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    Dokazivanje gena za enterotoksine koagulaza-negativnih stafilokoka iz autohtonih hrvatskih fermentiranih kobasica.

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    The aim of this study was to determine the presence of enterotoxin genes in coagulase - negative staphylococci from traditional Croatian fermented sausages. A total of 39 isolates were collected from the dry sausage ā€œkulenā€ and Slavonian homemade sausages as presumptive CNS, and were subjected to PCR identification to the genus level. All isolates belonged to the Staphylococcus genus. Strains were tested for the presence of 13 enterotoxin genes; seA, seB, seC, seG, seI, tsst1, seD, seE, seH, seJ, seM, seN and seO by the PCR method. All strains were negative to all enterotoxin genes. The observed results indicate the absence of risk related to enterotoxogenic potential of coagulase - negative staphylococci as indigenous microbiota from autochthonous Croatian traditional sausages.Cilj ovog rada bio je utvrditi prisutnost gena za enterotoksine u koagulaza - negativnih stafilokoka iz tradicionalnih hrvatskih fermentiranih kobasica. Ukupno je prikupljeno 39 izolata iz kulena i domaće slavonske kobasice te identificirano PCR - om do razine roda. Svi izolati pripadali su rodu Staphylococcus. PCR - om je određivana prisutnost 13 gena za enterotoksine: seA, seB, seC, seG, seI, tsst1, seD, seE, seH, seJ, seM, seN i seO. Ni u jednom izolatu nije utvrđena prisutnost gena za enterotoksine. Dobiveni rezultati upućuju na odsutnost rizika povezanog s toksogenim potencijalom koagulaza-negativnih stafilokoka kao dijela autohtone mikroflore hrvatskih fermentiranih kobasica

    Dokazivanje gena za enterotoksine koagulaza-negativnih stafilokoka iz autohtonih hrvatskih fermentiranih kobasica.

    Get PDF
    The aim of this study was to determine the presence of enterotoxin genes in coagulase - negative staphylococci from traditional Croatian fermented sausages. A total of 39 isolates were collected from the dry sausage ā€œkulenā€ and Slavonian homemade sausages as presumptive CNS, and were subjected to PCR identification to the genus level. All isolates belonged to the Staphylococcus genus. Strains were tested for the presence of 13 enterotoxin genes; seA, seB, seC, seG, seI, tsst1, seD, seE, seH, seJ, seM, seN and seO by the PCR method. All strains were negative to all enterotoxin genes. The observed results indicate the absence of risk related to enterotoxogenic potential of coagulase - negative staphylococci as indigenous microbiota from autochthonous Croatian traditional sausages.Cilj ovog rada bio je utvrditi prisutnost gena za enterotoksine u koagulaza - negativnih stafilokoka iz tradicionalnih hrvatskih fermentiranih kobasica. Ukupno je prikupljeno 39 izolata iz kulena i domaće slavonske kobasice te identificirano PCR - om do razine roda. Svi izolati pripadali su rodu Staphylococcus. PCR - om je određivana prisutnost 13 gena za enterotoksine: seA, seB, seC, seG, seI, tsst1, seD, seE, seH, seJ, seM, seN i seO. Ni u jednom izolatu nije utvrđena prisutnost gena za enterotoksine. Dobiveni rezultati upućuju na odsutnost rizika povezanog s toksogenim potencijalom koagulaza-negativnih stafilokoka kao dijela autohtone mikroflore hrvatskih fermentiranih kobasica
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