3 research outputs found
Preventive Measures against Speech Disorders in Early Childhood
AbstractThis article investigates the theoretical and practical aspects of speech disorders in early childhood. The article contains both the analysis of young children's speech diagnostics results and main aspects of a preventive kids’ program “I learn how to speak correctly”. The program is aimed at improving gross, fine and articulatory motor skills, psychical processes (memory, attention, thinking, perception), impressive and expressive kids’ speech, developing parents’ pedagogic behavior concerning the speech development problem in early childhood
\u3cem\u3e Limosilactobacillus Fermentum\u3c/em\u3e 3872 That Produces Class III Bacteriocin Forms Co-aggregates with the Antibiotic-resistant\u3cem\u3e Staphylococcus Aureus\u3c/em\u3e Strains and Induces Their Lethal Damage
LF3872 was isolated from the milk of a healthy lactating and breastfeeding woman. Earlier, the genome of LF3872 was sequenced, and a gene encoding unique bacteriocin was discovered. We have shown here that the LF3872 strain produces a novel thermolabile class III bacteriolysin (BLF3872), exhibiting antimicrobial activity against antibiotic-resistant Staphylococcus aureus strains. Sequence analysis revealed the two-domain structural (lysozyme-like domain and peptidase M23 domain) organization of BLF3872. At least 25% residues of this protein are expected to be intrinsically disordered. Furthermore, BLF3872 is predicted to have a very high liquid-liquid phase separation. According to the electron microscopy data, the bacterial cells of LF3872 strain form co-aggregates with the S. aureus 8325-4 bacterial cells. LF3872 produced bacteriolysin BLF3872 that lyses the cells of the S. aureus 8325-4 mastitis-inducing strain. The sensitivity of the antibiotic-resistant S. aureus collection strains and freshly isolated antibiotic-resistant strains was tested using samples from women with lactation mastitis; the human nasopharynx and oral cavity; the oropharynx of pigs; and the cows with a diagnosis of clinical mastitis sensitive to the lytic action of the LF3872 strain producing BLF3872. The co-cultivation of LF3872 strain with various antibiotic-resistant S. aureus strains for 24 h reduced the level of living cells of these pathogens by six log. The LF3872 strain was found to be able to co-aggregate with all studied S. aureus strains. The cell-free culture supernatant of LF3872 (CSLF3872) induced S. aureus cell damage and ATP leakage. The effectiveness of the bacteriolytic action of LF3872 strain did not depend on the origin of the S. aureus strains. The results reported here are important for the creation of new effective drugs against antibiotic-resistant strains of S. aureus circulating in humans and animals