Delay-based Passenger Car Equivalent at Signalized Intersections in Iran

Due to their different sizes and operational characteristics, vehicles other than passenger cars have a different influence on traffic operations especially at intersections. The passenger car equivalent (PCE) is the parameter that shows how many passenger cars must be substituted for a specific heavy vehicle to represent its influence on traffic operation. PCE is commonly estimated using headway-based methods that consider the excess headway utilized by heavy vehicles. In this research, the PCE was estimated based on the delay parameter at three signalized intersections in Tehran, Iran. The data collected were traffic volume, travel time for each movement, signalization, and geometric design information. These data were analysed and three different models, one for each intersection, were constructed and calibrated using TRAF-NETSIM simulation software for unsaturated traffic conditions. PCE was estimated under different scenarios and the number of approach movements at each intersection. The results showed that for approaches with only one movement, PCE varies from 1.1 to 1.65. Similarly, for approaches with two and three movements, the PCE varies from 1.07 to 1.99 and from 0.76 to 3.6, respectively. In addition, a general model was developed for predicting PCE for intersections with all of the movements considered. The results obtained from this model showed that the average PCE of 1.5 is similar to the value recommended by the HCM (Highway Capacity Manual) 1985. However, the predicted PCE value of 1.9 for saturated threshold is closer to the PCE value of 2 which was recommended by the HCM 2000 and HCM 2010.</p

Pericytes regulate vascular immune homeostasis in the CNS.

Pericytes regulate the development of organ-specific characteristics of the brain vasculature such as the blood-brain barrier (BBB) and astrocytic end-feet. Whether pericytes are involved in the control of leukocyte trafficking in the adult central nervous system (CNS), a process tightly regulated by CNS vasculature, remains elusive. Using adult pericyte-deficient mice (Pdgfb ret/ret ), we show that pericytes limit leukocyte infiltration into the CNS during homeostasis and autoimmune neuroinflammation. The permissiveness of the vasculature toward leukocyte trafficking in Pdgfb ret/ret mice inversely correlates with vessel pericyte coverage. Upon induction of experimental autoimmune encephalomyelitis (EAE), pericyte-deficient mice die of severe atypical EAE, which can be reversed with fingolimod, indicating that the mortality is due to the massive influx of immune cells into the brain. Additionally, administration of anti-VCAM-1 and anti-ICAM-1 antibodies reduces leukocyte infiltration and diminishes the severity of atypical EAE symptoms of Pdgfb ret/ret mice, indicating that the proinflammatory endothelium due to absence of pericytes facilitates exaggerated neuroinflammation. Furthermore, we show that the presence of myelin peptide-specific peripheral T cells in Pdgfb ret/ret ;2D2 tg mice leads to the development of spontaneous neurological symptoms paralleled by the massive influx of leukocytes into the brain. These findings indicate that intrinsic changes within brain vasculature can promote the development of a neuroinflammatory disorder

Inhibition of anti-tumor immunity by melanoma cell-derived Activin-A depends on STING

The transforming growth factor-β (TGF-β) family member activin A (hereafter Activin-A) is overexpressed in many cancer types, often correlating with cancer-associated cachexia and poor prognosis. Activin-A secretion by melanoma cells indirectly impedes CD8+ T cell-mediated anti-tumor immunity and promotes resistance to immunotherapies, even though Activin-A can be proinflammatory in other contexts. To identify underlying mechanisms, we here analyzed the effect of Activin-A on syngeneic grafts of Braf mutant YUMM3.3 mouse melanoma cells and on their microenvironment using single-cell RNA sequencing. We found that the Activin-A-induced immune evasion was accompanied by a proinflammatory interferon signature across multiple cell types, and that the associated increase in tumor growth depended at least in part on pernicious STING activity within the melanoma cells. Besides corroborating a role for proinflammatory signals in facilitating immune evasion, our results suggest that STING holds considerable potential as a therapeutic target to mitigate tumor-promoting Activin-A signaling at least in melanoma

Erosion Rate Prediction Model for Levee-Floodwall Overtopping Applications in Cohesive Soils

Floodwall overtopping erodes the levee surface. Characterizing the erosion rate of levee-floodwall systems is crucial for designing such structures. Scouring as a result of overtopping during storm events, is one of the major reasons for the failure of these structures. In this study, the contributing factors such was floodwall height, soil characteristics, and hydraulic characteristics of overtopped flow are considered to develop an erosion rate prediction model for such structures built with fine-grained soils. Using over 60 simulated scaled levee-floodwall test results, the effect of the contributing parameters on soil erosion described and quantified. The tests cover relatively wide ranges of soil plasticity index (0% to 40%), degree of compaction (70% to 90%), saturation ratio (15% to 85%), scale of the floodwall (1:20 to 1:2), and overtopped flow velocity (0.2 m/sec to 0.6 m/sec). The scouring mechanism in the soil with low plasticity indices (0% to 9%) developed scouring hole, and by increasing the saturation ratio, erosion rate increased significantly. However, in the soil samples with high plasticity indices (i.e., 30% and 40%) a scour hole development is less pronounced and erosion rates decrease by increasing the saturation ratio. Utilizing the lab-scaled simulation results, a prediction method was developed to estimate erosion rates based on other soil and hydraulic properties. The developed model was validated using case history and compared to other prediction methods

Evolution of Water Retention Characteristics in Bio-Geochemically Altered Unsaturated Soils: A Pore-Scale Study

Biogeochemical processes in subsurface can dramatically alter the behavior of multiphase fluid flow and the hydrodynamics of porous media by bio-clogging. There exist analytical solutions such as soilsuction – saturation equations which can be used to predict the water retention curve relations in unsaturated soils. However, due to the complexity of various biogeochemical products, their pore-scale behavior and their interplay with pore structure, such analytical solutions would not provide accurate predictions. In this study, a large database of pore-networks were generated by adjusting the statistical and spatial pore and tube size distribution of the networks resembling various levels of bio-clogging. Numerical simulations including the evolution of pore structure, water retention relationship, and air invasion dynamics during desaturationwere explored. The numerical simulations verified that local pore-clogging leads to the development of isolated pore clusters and impermeable zones. The evolution of impermeable zones results in the formation of preferential flow paths towards the mobile zones for the multiphase flow problems. Using parallel computation, the critical predictors of water retention curves in bio-clogged porous media are found

Molecular homology between canine spontaneous oral squamous cell carcinomas and human head-and-neck squamous cell carcinomas reveals disease drivers and therapeutic vulnerabilities

Spontaneously occurring canine oral squamous cell carcinomas (COSCC) are viewed as a useful model for human head and neck squamous cell carcinomas (HNSCC). To date however, the molecular basis of COSCC remains poorly understood. To identify changes pertinent to cancer cells in COSCC, we specifically analyzed tumor cells and matched normal epithelium from clinical formalin-fixed paraffin-embedded specimens using laser-capture-microdissection coupled with RNA-sequencing (RNAseq). Our results identify strong contributions of epithelial-to-mesenchymal transition (EMT), classical tumor-promoting (such as E2F, KRAS, MYC, mTORC1, and TGFB1 signaling) and immune-related pathways in the tumor epithelium of COSCC. Comparative analyses of COSCC with 43 paired tumor/normal HNSCC from The Cancer Genome Atlas revealed a high homology in transcriptional reprogramming, and identified processes associated with cell cycle progression, immune processes, and loss of cellular differentiation as likely central drivers of the disease. Similar to HNSCC, our analyses suggested a ZEB2-driven partial EMT in COSCC and identified selective upregulation of KRT14 and KRT17 in COSCC. Beyond homology in transcriptional signatures, we also found therapeutic vulnerabilities strongly conserved between the species: these included increased expression of PD-L1 and CTLA-4, coinciding with EMT and revealing the potential for immune checkpoint therapies, and overexpression of CDK4/6 that sensitized COSCC to treatment with palbociclib. In summary, our data significantly extend the current knowledge of molecular aberrations in COSCC and underline the potential of spontaneous COSCC as a model for HNSCC to interrogate therapeutic vulnerabilities and support translation of novel therapies from bench to bedside

Incorporating car owner preferences for the introduction of economic incentives for speed limit enforcement

Human error including driving misbehavior contributes to over 90 percent of road vehicle accidents, and speeding is considered to be risky. Smart technologies, such as Connected Vehicle System (CVS) are among the interesting technical options to improve driving behavior, and Pay-As-You-Speed (PAYS) is an effective economic incentive to reduce speed violations. We investigated the acceptability of CVS with and without the presence of economic incentives, such as PAYS, in the context of a middle-income country: Iran. We used a Zero-Inflated Ordered Probit model (ZIOP) to estimate drivers’ willingness to pay for a CVS, and a hazard-based model for predicting the incentive level needed for accepting CVS via a PAYS scheme. ZIOP model indicated that drivers with the following characteristics were more likely to pay more for CVS: having a comprehensive insurance coverage, being younger than 60 years, owning more than one car, and having older vehicles. The hazard-based model also confirmed that drivers that speed relatively often have a lower tendency to adopt CVS, and drivers who experienced an accident in the past were more inclined to adopt CVS via PAYS. Also, drivers' opinion about CVS, vehicle characteristics, demographics, and driving experience influenced the effect of PAYS characteristics on acceptability of CVS. Finally, we offer recommendations for how to effectively implement CVS, in order to significantly reduce the high fatality and accident rates in middle-income countries such as Iran.</p

Effect of Sediment Accumulation on Best Management Practice (BMP) Stormwater Runoff Volume Reduction Performance for Roadways

Best management practices (BMPs) are commonly used to reduce the adverse effects of post-construction runoff. BMP deterioration happens over time when these age and the infiltration rate decreases as a result of sediment accumulation. The objective of this paper was to investigate the effect of sediment accumulation on BMP stormwater runoff volume reduction performances. The BMPs studied included a bioswale and an infiltration trench. To undertake this research, both field tests and numerical simulations were conducted under five different and single rainfall events with a wide range of intensities and duration. The minimal sediment accumulation of 0.22 kg/m2·year was considered in this study. Three different sedimentation accumulation configurations (i.e., new, 2-year-old, and 10-year-old BMPs) were considered. According to the results, the infiltration trench had 100% runoff reduction efficiency in all conditions including high-intensity rain and 10-year-old BMP age. The performance of the bioswale for the first 2 and 10 years deteriorated by about 55% and 70%, respectively

Correlates of self-reported driving aberrations in Tehran: A study at the level of drivers and districts

There are relatively few comprehensive studies on driving errors and violations in Iran, a non-Western country with a high traffic fatality rate. In this study, 712 drivers completed a questionnaire at technical inspection centres and carwashes in Tehran, Iran. Respondents were asked about their demographic characteristics, accident involvement, traffic fines, and driving aberrations in the form of the Driver Behaviour Questionnaire (DBQ). The results of a principal component analysis of the DBQ showed a distinction between errors and two types of violations: speeding and non-speeding violations. Correlation analyses showed that DBQ violations were associated with a higher driving mileage, a higher education level (for DBQ speeding violations in particular), and younger age. DBQ errors were associated with risk perception, that is, the belief that one has a high probability of becoming involved in a car accident. Regression analyses showed that the DBQ speeding violations score was predictive of the number of speeding tickets and that the DBQ non-speeding violations score was predictive of involvement in minor accidents in the past three years. A correlation analysis at the level of municipal districts showed that drivers from districts with lower education and literacy levels and lower car ownership were more likely to report driving a low-cost car and had lower DBQ violations scores. These results can be interpreted as indicating that affluence enables deviant driving. We conclude that the error-violation distinction is of relevance to road safety in Tehran, both at the level of individual drivers and at the level of districts.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Human-Robot Interactio