Fluorescence based detection of bioaerosols to improve emissions characterization from environmental sources

Bioaerosols are ubiquitous in ambient air but there have been increasing concerns about their human exposure and to health impact due to ever increasing environmental emissions from sources such as biowaste and intensive agriculture facilities (Borlée et al. 2015). However, the knowledge on their risk of exposure to the public is limited mainly due to a lack of emission characterisation, in part due to the limitation of conventional methods for the detection and characterisation of ambient bioaerosols. Among emerging techniques, fluorescence spectroscopy has shown promise in detecting and broadly classifying bioaerosols (Pan et al. 2015). This paper provides the preliminary results of a study that aims to demonstrate the potential of a fluorescence based bioaerosol sensor unit to detect and quantify these in real time with a view to developing and advancing bioaerosol exposure assessment methodologies to various environmental sources

Routine Testing of Liver Function Before and After Elective Laparoscopic Cholecystectomy: Is It Necessary?

This study suggests that mild to moderate elevation in preoperative liver function tests may not be associated with deleterious effects, and in the absence of clinical indications, routine preoperative or postoperative liver function testing may not be necessary

Effects of Cooperative Learning Models on Prospective Teachers’ Achievement and Equity

The cooperative learning (CL) intervention was conducted to find out the effects of two conditions of cooperative learning on prospective teachers (PTs) achievement and equity. Cooperative learning was embedded in methods of teaching English course. Thirty five students enrolled in Masters programme (MA ELTL) were the subjects of the study. The study employed counter balanced design. The main research questions addressed were; (1) What is the difference in PTs achievement after exposure to CL as compared to their previous CGPA? (2) Which model of CL is better for producing higher achievement? (3) Which CL model provides/ establishes equity (equal chances to succeed) to maximum number of students? Achievement tests and class quizzes were administered to obtain data. Paired samples t-test, Mann-Whitney U test and Kruskal-Wallis test were carried out to answer the research questions. The findings suggest that CL significantly enhances PTs achievement as compared to TI. Both CL models are compatible to increase achievement and equity in TE. Learning together is more efficient and effective as compared to STAD. The study open doors for doing innovations and intervention in teacher education through different CL models

Particulate pollution in different housing types in a UK suburban location

To investigate the levels of particulate pollution in residential built environments measurements of PM10, PM2.5, and PM1 and concentrations were made between 2004 and 2008 in various residencies in a UK suburban location. Measurements were carried out in three different residential settings (Types I, II and III). In type I non-smoking living rooms, the highest 24-hour mean concentrations were found in summer. When smoking took place in type I residences, the concentrations of PM10, PM2.5 and PM1, during the winter were almost double those in summer. In type II houses the concentrations were higher in the houses with open plan kitchens than in those with separate kitchens. In type III houses, mean concentrations were significantly higher in wood heated living rooms than those using central heating. In kitchens, cooking resulted in substantially higher concentrations of particulate matter with levels above those in smoking living rooms in winter. The hourly maximum values of number concentration were considerably higher in smoking rooms than non-smoking ones. Cooking resulted in increased number concentrations, with the average hourly maximum concentration of 179,110 #/cm3. Particle mass and number emission rates were determined for a number of activities. In kitchens grilling had the highest average number emission rate, followed by boiling and frying. The results clearly highlight the impact of different forms of dwelling and their use and management by occupants on the levels of particulate matter in naturally ventilated residential built environments. © 2012 Elsevier B.V

Indoor particulate matter in developing countries: a case study in Pakistan and potential intervention strategies

Around three billion people, largely in low and middle income countries, rely on biomass fuels for their household energy needs. The combustion of these fuels generates a range of hazardous indoor air pollutants and is an important cause of morbidity and mortality in developing countries. Worldwide, it is responsible for four million deaths. A reduction in indoor smoke can have a significant impact on lives and can help achieve many of the Millennium Developments Goals. This letter presents details of a seasonal variation in particulate matter (PM) concentrations in kitchens using biomass fuels as a result of relocating the cooking space. During the summer, kitchens were moved outdoors and as a result the 24 h average PM10, PM2.5 and PM1 fell by 35%, 22% and 24% respectively. However, background concentrations of PM10 within the village increased by 62%. In locations where natural gas was the dominant fuel, the PM concentrations within the kitchen as well as outdoors were considerably lower than those in locations using biomass. These results highlights the importance of ventilation and fuel type for PM levels and suggest that an improved design of cooking spaces would result in enhanced indoor air quality. © 2013 IOP Publishing Ltd

Microbial dynamics during various activities in residential areas of Lahore, Pakistan

Bioaerosols are ubiquitous in the atmosphere with their levels affected by a variety of environmental factors as well as type of activities being carried out at any specific time. The present study investigated how indoor activities influence bioaerosol concentrations in five residential houses of Lahore. Agar coated petri plates were exposed face upwards for twenty minutes in kitchens and living rooms during activity and non-activity periods. The temperature and relative humidity levels were noted as well. The bioaerosol concentrations in kitchens during the activity time ranged between 1022 to 4481 cfu/m3 and in living rooms from 1179 to 3183 cfu/m3 . Lower values were observed during non-activity periods. A paired-t test revealed a significant difference in bacterial loads during activity and non-activity times in both micro-environments (p = 0.038 in kitchen and p = 0.021 in living room). The predominant species identified were Micrococcus spp., Staphylococcus spp., and Bacillus spp. which are a common constituent of the indoor environment and are known to be opportunistic pathogens as well

Measurement of NO2 indoor and outdoor concentrations in selected public schools of Lahore using passive sampler

Higher levels of NO2 are a danger to human health especially for children. A seven day study was carried to find out the ambient concentrations of NO2in 27 schools of Lahore with the help of passive samplers. In each school three sites were selected, viz: laboratory, corridor and outdoors. After 7 days exposure the tubes were subjected to spectrophotometric analysis. Results showed that the maximum values measured in laboratory, outdoor and corridors were 376µg/m3 , 222µg/m3 and 77µg/m3 . Minimum values for laboratory, outdoor and corridors were 10µg/m3 , 20µg/m3 and 8µg/m3 . Factors affecting these values were laboratory activities and proximity to main roads. These values were significantly higher than the standard values defined by EPA. Therefore children in schools were at risk of developing health complications

Bioaerosols in residential micro-environments in low income countries: A case study from Pakistan

Our knowledge of the concentrations of bioaerosols in residential micro-environments in low income countries is scanty. The present investigation was conducted to assess the culturable concentration and size distribution of bacteria, gram negative bacteria and fungi in two rural and an urban site in Pakistan. The highest indoor culturable bacteria concentration was found at Rural Site II (14,650 CFU/m 3) while the outdoor maximum occurred at the urban site (16,416 CFU/m 3). With reference to fungi, both indoor and outdoor concentrations were considerably higher at Rural Site I than the other sites. The size distribution of culturable bacteria at all sites showed greater variability than that of culturable fungi. At all sites more than the half (55-93%) of the culturable bacterial and fungal counts were observed in the respirable fraction (<4.7 μm) and so had the potential to penetrate into lower respiratory system. © 2012 Elsevier Ltd. All rights reserved

Assessment of airborne particulate matter (PM2.5) in university classrooms of varying occupancy

Air pollution is a major concern in Pakistan. Levels of particulate matter (PM2.5) in educational built environments, have not yet been studied comprehensively in Pakistan. This study was conducted to assess relationships between indoor and outdoor particulate matter in classrooms of the University of the Punjab, Lahore, using a DUSTTRAK Aerosol Monitor (TSI Model 8520). Sampling for PM2.5concentrations was carried out simultaneously outdoors and indoors in different classrooms on the campus. According to the level of occupancy three classrooms were selected i.e. Classroom I: low occupancy, Classroom II: medium occupancy and Classroom III: high occupancy. Simultaneous outdoor measurements were carried out at rooftop of each classroom. A tracer method was used to measure the air change per hour in each classroom. The 24 hour average concentrations of PM2.5 in Classrooms I, II and III were observed to be 282 .g/m³, 75 .g/m³ and 673 .g/m³ whereas 24 hour average outdoor levels were 324 .g/m³, 121 .g/m³ and 998.g/m³ respectively. Results showed a significant impact of ambient air and occupancy level on PM2.5 levels inside classrooms and all observed values exceeded the WHO limit

Airborne biological hazards and urban transport infrastructure: current challenges and future directions

Exposure to airborne biological hazards in an ever expanding urban transport infrastructure and highly diverse mobile population is of growing concern, in terms of both public health and biosecurity. The existing policies and practices on design, construction and operation of these infrastructures may have severe implications for airborne disease transmission, particularly, in the event of a pandemic or intentional release of biological of agents. This paper reviews existing knowledge on airborne disease transmission in different modes of transport, highlights the factors enhancing the vulnerability of transport infrastructures to airborne disease transmission, discusses the potential protection measures and identifies the research gaps in order to build a bioresilient transport infrastructure. The unification of security and public health research, inclusion of public health security concepts at the design and planning phase, and a holistic system approach involving all the stakeholders over the life cycle of transport infrastructure hold the key to mitigate the challenges posed by biological hazards in the twenty-first century transport infrastructure