Effect of Fermented Camel Milk on Obesity Measures and Blood Pressure of Adolescents With Metabolic Syndrome

Background: Metabolic Syndrome (MetS) predisposes the human body to a variety of chronic diseases.  Objectives: This study, for the first time, aimed to assess the effects of Fermented Camel Milk (FCM), a functional dairy food, on the obesity measures and blood pressure of adolescents with MetS. Methods: This was a crossover, randomized, double-blind trial. We enrolled overweight or obese adolescents, aged 11-18 years, meeting the diagnostic criteria for MetS. We randomly assigned the study participants to receive FCM 250 mL per day for 8 weeks, followed by a 4-week washout, then, consuming Diluted Cow Yogurt (DCY) 250 mL per day for 8 weeks, or the reverse sequence. General and abdominal obesity measures consisting of weight, Body Mass Index (BMI), BMI z-score, Waist Circumference (WC), Hip Circumference (HC), waist to height ratio and Systolic and Diastolic Blood Pressure (SBP, DBP) were measured before and after each of the 4 periods. A three-day food record and physical activity questionnaire were completed before each period. Statistical analyses were performed using Minitab and SPSS considering the significance level of 0.05. Results: Twenty-four participants with the Mean±SD age of 13.77±1.87 years (age range: 10.45-16.25 years) (58% girls) completed the study. It resulted in nonsignificant mean changes (increase or decrease) in weight of -0.67 kg (95%CI: -1.97; 0.61; P=0.28), BMI (-0.10 kg/m2, 95%CI: -0.65; 0.45; P=0.70), BMI z-score of -0.06 (95%CI: -0.33; 0.19; P=0.59), WC of -1.10 cm (95%CI: -3.22; 1.01; P=0.29), and HC of -0.12 cm (95%CI: -2.04; 1.79; P=0.89) by FCM consumption in comparison to DCY. The study also resulted in non-significant mean reduction in DBP of -4.45 mm Hg (95%CI: -10.04; 1.12; P=0.11).  Conclusions: According to some positive impacts of FCM on obesity measures and blood pressure, we suggest conducting further studies to validate the clinical impacts of fermented camel milk

Paediatric pre-B acute lymphoblastic leukaemia-derived exosomes regulate immune function in human T cells

Exosomes derived from solid tumour cells are involved in immune suppression, angiogenesis and metastasis; however, the role of leukaemia-derived exosomes has less been investigated. Hence, changes in immune response-related genes and human T cells apoptosis co-incubated with exosomes isolated from patients' pre-B cell acute lymphoblastic leukaemia were evaluated in this in vitro study. Vein blood sample was obtained from each newly diagnosed acute lymphoblastic leukaemia (ALL) patient prior any therapy. ALL serum exosomes were isolated by ultrafiltration and characterized using Western blotting and transmission electron microscopy. Exosomes were then co-incubated with T lymphocytes and the gene expressions, as well as functions of human T cells were quantified by qRT-PCR. Apoptosis and caspase-3 and caspase-9 protein expression were also evaluated by flowcytometry and Western blotting analysis, respectively. Exosomes isolated from ALL patients affected T lymphocytes and elevated the apoptosis. Moreover, these exosomes altered the T cells profile into regulatory type by increasing the expression of FOXP3 and Tregs-related cytokines, including TGF-B and IL-10. The expression level of Th17-related transcription factors (RoRγt) and interleukins (IL-17 and IL-23) decreased after this treatment. According to our findings, exosomes derived from ALL patients' sera carry immunosuppressive molecules, indicating the possible effect of exosomes as liquid biomarkers for cancer staging

Paediatric pre-B acute lymphoblastic leukaemia-derived exosomes regulate immune function in human T cells

Exosomes derived from solid tumour cells are involved in immune suppression, angiogenesis and metastasis; however, the role of leukaemia‐derived exosomes has less been investigated. Hence, changes in immune response‐related genes and human T cells apoptosis co‐incubated with exosomes isolated from patients' pre‐B cell acute lymphoblastic leukaemia were evaluated in this in vitro study. Vein blood sample was obtained from each newly diagnosed acute lymphoblastic leukaemia (ALL) patient prior any therapy. ALL serum exosomes were isolated by ultrafiltration and characterized using Western blotting and transmission electron microscopy. Exosomes were then co‐incubated with T lymphocytes and the gene expressions, as well as functions of human T cells were quantified by qRT‐PCR. Apoptosis and caspase‐3 and caspase‐9 protein expression were also evaluated by flowcytometry and Western blotting analysis, respectively. Exosomes isolated from ALL patients affected T lymphocytes and elevated the apoptosis. Moreover, these exosomes altered the T cells profile into regulatory type by increasing the expression of FOXP3 and Tregs‐related cytokines, including TGF‐B and IL‐10. The expression level of Th17‐related transcription factors (RoRγt) and interleukins (IL‐17 and IL‐23) decreased after this treatment. According to our findings, exosomes derived from ALL patients' sera carry immunosuppressive molecules, indicating the possible effect of exosomes as liquid biomarkers for cancer staging