Polymorphisms in the selectin gene cluster are associated with fertility and survival time in a population of Holstein Friesian cows

Selectins are adhesion molecules, which mediate attachment between leucocytes and endothelium. They aid extravasation of leucocytes from blood into inflamed tissue during the mammary gland’s response to infection. Selectins are also involved in attachment of the conceptus to the endometrium and subsequent placental development. Poor fertility and udder health are major causes for culling dairy cows. The three identified bovine selectin genes SELP, SELL and SELE are located in a gene cluster. SELP is the most polymorphic of these genes. Several SNP in SELP and SELE are associated with human vascular disease, while SELP SNP rs6127 has been associated with recurrent pregnancy loss in women. This study describes the results of a gene association study for SNP in SELP (n = 5), SELL (n = 2) and SELE (n = 1) with fertility, milk production and longevity traits in a population of 337 Holstein Friesian dairy cows. Blood samples for PCR-RFLP were collected at 6 months of age and animals were monitored until either culling or 2,340 days from birth. Three SNP in SELPEx4-6 formed a haplotype block containing a Glu/Ala substitution at rs42312260. This region was associated with poor fertility and reduced survival times. SELPEx8 (rs378218397) coded for a Val475Met variant locus in the linking region between consensus repeats 4 and 5, which may influence glycosylation. The synonymous SNP rs110045112 in SELEEx14 deviated from Hardy Weinberg equilibrium. For both this SNP and rs378218397 there were too few AA homozygotes present in the population and AG heterozygotes had significantly worse fertility than GG homozygotes. Small changes in milk production associated with some SNP could not account for the reduced fertility and only SELPEx6 showed any association with somatic cell count. These results suggest that polymorphisms in SELP and SELE are associated with the likelihood of successful pregnancy, potentially through compromised implantation and placental development

Results of parodontium treatment related to platelet-reach plasma

In current moment of problem treatment parodontium is actual. We have offered do inject platelet-reach plasma after hygiene professional of cavities mouth locked in pleat connecting, 0,3 ml in one segment tooth including thee teeth. Results of investigation have shown through one year in group main of patients under using platelet-reach plasma they were noted realistically more low factors of indexes parodontium and cellumorphometry under comparison with group checking

Structural Properties of Ribosomal Protein S8 from the Extreme Thermophile Thermus Thermophilus

The gene of ribosomal protein S8 from the extreme thermophile Thermus thermophilus was expressed in E. coli using the strain BL21(DE3) and vector pET3-1. A method of isolating this protein from the super producing strain was developed, which makes it possible to obtain 8-12 mg of product from 11 of culture. The secondary structure of protein S8 was determined by using CD spectroscopy. The protein was shown to be highly resistant to denaturants

Structural Properties of Ribosomal Protein S8 from the Extreme Thermophile Thermus Thermophilus

The gene of ribosomal protein S8 from the extreme thermophile Thermus thermophilus was expressed in E. coli using the strain BL21(DE3) and vector pET3-1. A method of isolating this protein from the super producing strain was developed, which makes it possible to obtain 8-12 mg of product from 11 of culture. The secondary structure of protein S8 was determined by using CD spectroscopy. The protein was shown to be highly resistant to denaturants