Antibacterial Activities of Kelussia odoratissima and Teucrium polium Essential Oils in Combination of Synthetic Silver Nanoparticles against Food- borne Pathogens

To study the interaction of silver nanoparticles with antibacterial effects of Kelussia odoratissima and Teucrium polium essential oils consolidated trial based on calculation of MIC and FIC index or differential inhibitory concentration and simple method checkboard designed and implemented. Essential oil of the plants extracted by hydro-distillation using clevenger apparatus. In this study, common food contaminant bacteria such as Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Escherichia coli O157: H7, Salmonella enterica and Ps. aureogenosa were used. The results showed that the antibacterial properties of the essential oil of Teucrium is stronger (MIC between 0.16 to 1.25 mg/ ml) than of the oil of Kelussia (MIC between 0.3 to 2.5 mg/ml). Antibacterial properties of silver nanoparticles (MIC between 0.006 to 0.025 mg) were stronger significantly. Calculate the differential inhibitory concentration index (FICi) implies the existence of synergistic and additive effect between silver nanoparticles and oil of both plant which will depend on the type of pathogen. This means that the combined use of silver nanoparticles and essential oil of the plant especially Teucrium essential oils strong inhibitory effect with lower concentrations of the essential oils was observed. Antagonistic interaction between silver nanoparticles and oil treatments in any of these two plants were observed