Effect of Estradiol Benzoate on the Size of Follicle and Corpus Luteum of Holstein Cows Treated with D-Cloprostenol.

The effect of two treatments, farms, and body  shape on the size of the follicle and  Corpus Luteum  were evaluated. D-Cloprostenol (150 ug) was used in one of the treatments; in the other,  D-Cloprostenol (150 ug) and Estradiol Ben-zoate (1 mg), were used. Two groups of 32 Holstein dairy cows each, from the Nero and Irquis farms in Cuenca, Ec-uador, were set up.  Body condition  (2.75-3.50) was considered. The calving number was 1-6; the farms,  Irquis and Nero; and animal age, 3-10 years. Ultrasound scanning was used on days 0; 3 and 7, in order to measure the  Luteumfirst, then the  dominant ovarian follicle, and finally, the Corpus Luteum.  Factor analysis of variance  was  performed. No significant differences were found for follicle size due to any factors; nor due to interaction.  Concerning luteum, significant differences (P < 0.05) were observed for the treatments and for the farm. The values were 0.33 cm higher for the treatment with Benzoate, and 0.36 cm,  at  Irquis, in comparison to Nero. The treatment with Benzoate, along with better husbandry at Irquis played a critical role in the appearance and evolution of the luteum

Efecto del Benzoato de Estradiol en el tamaño del folículo y cuerpo lúteo en vacas Holstein sincronizadas con D-Cloroprostenol.

Se evaluó el efecto de dos tratamientos,  las granjas y la condición corporal en el tamaño del folículo y cuerpo lúteo. En uno de los tratamientos se aplicó D-Cloroprostenol (150 ug) y en el otro, D-Cloroprostenol (150 ug) y Ben-zoato de Estradiol (1mg). Se formaron dos grupos de 32 vacas Holstein cada uno, pertenecientes a las granjas Nero e Irquis, en Cuenca, Ecuador. Se tomó en cuenta la condición corporal (2,75-3,50), el número de partos fue de 1-6, las granjas (Irquis y Nero) y edad de los animales (3-10 años). Se usó ecografía a 0;  3 y 7 días, así para en el primer momento medir el cuerpo lúteo, luego el folículo dominante y la última el cuerpo  lúteo. Se realizó un análisis de va-rianza factorial. No se encontraron diferencias significativas para el tamaño del folículo por efecto de ninguno de los factores ni la interacción, no así en el tamaño del cuerpo lúteo, donde se registraron diferencias s ignificativas (P < 0,05) para los tratamientos y para la granja. El valor fue superior en 0,33 cm en el tratamiento con Benzoato y de 0,36 cm en la granja Irquis respecto a Nero. La aplicación del tratamiento con Benzoato y el mejor manejo de la granja Irquis tuvieron influencia importante en la formación y desarrollo del cuerpo lúteo.Effect of Estradiol Benzoate on the Size of Follicle and Corpus Luteum of Holstein Cows, Treated with D-Cloprostenol.ABSTRACTThe effect of two treatments, farms, and body shape on the size of the follicle and Corpus Luteum, were evaluated. D-Cloprostenol (150 ug) was used in one of the treatments; in the other, D-Cloprostenol (150 ug) and Estradiol Ben-zoate (1mg), were used.  Two groups of 32 Holstein cows each,  from Nero and  Irquis farms in Cuenca, Ecuador, were set up.  Body condition  (2.75-3.50) was considered. The calving number was 1-6, the farms (Irquis and Nero), and animal age (3-10 years). Ultrasound scanning was used on days 0; 3 and 7, in order to measure the Luteum  first, then the dominating follicle, and finally, the Corpus Luteum. Factor variance analysis was performed. No significant differences were found for follicle size due to any factors; nor due to interaction. Concerning luteum, significant differences (P < 0.05) were observed for the treatments and for  the farm. The values were 0.33 cm higher for the treat-ment with Benzoate, and 0.36 cm, on Irquis Farm, in comparison to Nero. The treat ment with Benzoate, along with better husbandry on Irquis played a critical role in the appearance and evolution of the luteum