Initial Experience With Single-Isocenter Radiosurgery to Target Multiple Brain Metastases Using an Automated Treatment Planning Software: Clinical Outcomes and Optimal Target Volume Margins Strategy

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A Flavone-Based Solvatochromic Probe with A Low Expected Perturbation Impact on the Membrane Physical State

The study of the cell membrane is an ambitious and arduous objective since its physical state is regulated by a series of processes that guarantee its regular functionality. Among the different methods of analysis, fluorescence spectroscopy is a technique of election, non-invasive, and easy to use. Besides, molecular dynamics analysis (MD) on model membranes provides useful information on the possibility of using a new probe, following its positioning in the membrane, and evaluating the possible perturbation of the double layer. In this work, we report the rational design and the synthesis of a new fluorescent solvatochromic probe and its characterization in model membranes. The probe consists of a fluorescent aromatic nucleus of a 3-hydroxyflavone moiety, provided with a saturated chain of 18 carbon atoms and a zwitterionic head so to facilitate the anchoring to the polar heads of the lipid bilayer and avoid the complete internalization. It was possible to study the behavior of the probe in GUV model membranes by MD analysis and fluorescence microscopy, demonstrating that the new probe can efficiently be incorporated in the lipid bilayer, and give a color response, thanks to is solvatochromic properties. Moreover, MD simulation of the probe in the membrane supports the hypothesis of a reduced perturbation of the membrane physical state

Characterization of Silter Cheeses Produced in Valley and Alpine Pastures by a Proteomic Approach.

Silter is an Italian hard cheese manufactured with milk produced by cows fed at different altitude, valley or alpine pasture. The chemical, rheological and sensory properties of cheeses can be affected by the modification in milk composition due to the breed which at different altitude causes the modification of protein content, κ-CN glycosylation, plasmin activity, and coagulation properties. The influence of milk plasmin activity on dairy production was investigated in seven Silter cheeses, four produced in the valley and three from alpine mountain, through alkaline urea-polyacrylamide gel electrophoresis; two-dimensional gel electrophoresis coupled to mass spectrometry and image analysis. Results demonstrated that Silter cheese obtained from cows reared in alpine pasture is characterized by a more evident proteolysis, determining high levels of β-CN and αs1-CN fragments. Therefore, the most relevant fragmentation was attributed to a more intense activity of plasmin and to a different dosage of rennet to make up for the reduced coagulation properties of alpine milk

The Hubble Space Telescope UV Legacy Survey of Galactic Globular Clusters. XXIII. Proper-motion Catalogs and Internal Kinematics

A number of studies based on the data collected by the Hubble Space Telescope (HST) GO-13297 program "HST Legacy Survey of Galactic Globular Clusters: Shedding UV Light on Their Populations and Formation" have investigated the photometric properties of a large sample of Galactic globular clusters and revolutionized our understanding of their stellar populations. In this paper, we expand upon previous studies by focusing our attention on the stellar clusters' internal kinematics. We computed proper motions for stars in 56 globular clusters and one open cluster by combining the GO-13297 images with archival HST data. The astrophotometric catalogs released with this paper represent the most complete and homogeneous collection of proper motions of stars in the cores of stellar clusters to date, and expand the information provided by the current (and future) Gaia data releases to much fainter stars and into the crowded central regions. We also census the general kinematic properties of stellar clusters by computing the velocity dispersion and anisotropy radial profiles of their bright members. We study the dependence on concentration and relaxation time, and derive dynamical distances. Finally, we present an in-depth kinematic analysis of the globular cluster NGC 5904