Research protocol: essential stage of research process guaranteeing validity of results

La recherche scientifique est une activité obligatoire à tout enseignant à l’université. Nous avons réalisé ce travail de synthèse en apportant des innovations dans les composantes d’un protocole de recherche. Le but de notre travail est de rappeler aux chercheurs de tous les domaines, la démarche à suivre pour rédiger un bon projet de recherche encore appelé protocole de recherche. Un protocole de recherche complet doit contenir les onze composantes suivantes : le titre ; l’introduction ; la problématique ; l’hypothèse ; les objectifs ; les généralités ; la méthode ; le calendrier ; le budget ; la bibliographie et la mention des liens d’intérêt. La méthode d’étude est la partie essentielle du protocole de recherche. En conclusion, tout chercheur doit maîtriser la procédure d’élaboration d’un protocole de recherche lui permettant d’aboutir à des résultats valides et de publier des articles scientifiques de bonne qualité.Scientific research is a compulsory activity for every university teacher. We realized this synthetic work by bringing innovations in research protocol components. The purpose of our work is to remind the searchers in all domains, the approach to be followed to draft a good research project also called research protocol. A complete research protocol has to include the eleven following components: title, introduction, problem, hypothesis, objectives, majorities, method, timetable, budget, bibliography and conflict of interests. The method of study is an essential part of the research protocol. As a conclusion, we can say every searcher should know and be able to elaborate a research protocol in order to obtain valid results and to publish quality scientific articles

Effect of Topical Application of Hydro-Ethanolic Extract of Acacia nilotica Pods on Second-Degree Cutaneous Infected Burns

Acacia nilotica is a medicinal plant used traditionally in Togo to treat various diseases including burn wounds. The aim of this study was to evaluate experimentally Acacia nilotica burn wound healing effect.Cutaneous burn wounds were symmetrically caused on depilated areas of rat skin through contact with an aluminum bar (r = 10 mm), preheated at 80°C for 30 sec. Five groups of animal were constituted and each group contained 8 mice. Four groups of animal’s burn wounds were infected by Staphylococcus aureus. One group burn wound is uninfected and serves as negative control. Burn wounds were assessed by planimetry and histological parameters of healing. Twelve days after burn wound induction, wound contraction in the uninfected groups (negative control) was 19.9% for topical application against -2.43% for infected control. In the infected groups treated with extract, wound contraction was generally stimulates. Histological examination showed granulated tissue developing over the wounds treated with the extract of A. nilotica at 2.5% and 5% where the proliferation of fibroblasts and neo-blood vessels was very marked.In conclusion, A. nilotica pod contents tannin, flavonoids, alkaloids and protein reduces DPPH solution and significantly accelerates wound healing of burns, and this is the case even if wounds are infected with 109CFU/mL of S. aureus

Effect of Topical Application of Hydro-Ethanolic Extract of Acacia nilotica Pods on Second-Degree Cutaneous Infected Burns

Acacia nilotica is a medicinal plant used traditionally in Togo to treat various diseases including burn wounds. The aim of this study was to evaluate experimentally Acacia nilotica burn wound healing effect.Cutaneous burn wounds were symmetrically caused on depilated areas of rat skin through contact with an aluminum bar (r = 10 mm), preheated at 80°C for 30 sec. Five groups of animal were constituted and each group contained 8 mice. Four groups of animal’s burn wounds were infected by Staphylococcus aureus. One group burn wound is uninfected and serves as negative control. Burn wounds were assessed by planimetry and histological parameters of healing. Twelve days after burn wound induction, wound contraction in the uninfected groups (negative control) was 19.9% for topical application against -2.43% for infected control. In the infected groups treated with extract, wound contraction was generally stimulates. Histological examination showed granulated tissue developing over the wounds treated with the extract of A. nilotica at 2.5% and 5% where the proliferation of fibroblasts and neo-blood vessels was very marked.In conclusion, A. nilotica pod contents tannin, flavonoids, alkaloids and protein reduces DPPH solution and significantly accelerates wound healing of burns, and this is the case even if wounds are infected with 109CFU/mL of S. aureus