The Relationship between Uterine, Fecal, Bedding, and Airborne Dust Microbiota from Dairy Cows and Their Environment: A Pilot Study

Simple Summary After calving, dairy cows face the risk of negative energy balance, inflammation, and immunosuppression, which may result in bacterial infection and disruption of the normal microbiota, thus encouraging the development of metritis and endometritis. This study characterized uterine, fecal, bedding, and airborne dust microbiota from postpartum dairy cows and their environment during summer and winter. The results clarify the importance of microbiota in cowshed environments, i.e., bedding and airborne dust, in understanding the postpartum uterine microbiota of dairy cows. Abstract The aim of this study was to characterize uterine, fecal, bedding, and airborne dust microbiota from postpartum dairy cows and their environment. The cows were managed by the free-stall housing system, and samples for microbiota and serum metabolite assessment were collected during summer and winter when the cows were at one and two months postpartum. Uterine microbiota varied between seasons; the five most prevalent taxa were Enterobacteriaceae, Moraxellaceae, Ruminococcaceae, Staphylococcaceae, and Lactobacillaceae during summer, and Ruminococcaceae, Lachnospiraceae, Bacteroidaceae, Moraxellaceae, and Clostridiaceae during winter. Although Actinomycetaceae and Mycoplasmataceae were detected at high abundance in several uterine samples, the relationship between the uterine microbiota and serum metabolite concentrations was unclear. The fecal microbiota was stable regardless of the season, whereas bedding and airborne dust microbiota varied between summer and winter. With regards to uterine, bedding, and airborne dust microbiota, Enterobacteriaceae, Moraxellaceae, Staphylococcaceae, and Lactobacillaceae were more abundant during summer, and Ruminococcaceae, Lachnospiraceae, Bacteroidaceae, and Clostridiaceae were more abundant during winter. Canonical analysis of principal coordinates confirmed the relationship between uterine and cowshed microbiota. These results indicated that the uterine microbiota may vary when the microbiota in cowshed environments changes

アンシン カード オ モチイタ ガン カンジャ ノ キュウキュウ イリョウ タイセイ ノ コウチク ト ビョウビョウ ビョウシン レンケイ ノ ココロミ

Oncologic Emergency Medical（OCM）card is to guarantee medical care to be provided by Tokushima Municipal Hospital to advanced cancer patients who once receive medical care in the institution even after they are referred to other hospitals or when their medical condition is worsened. Forty-seven cancer patients have been issued with the OCM card between April２０１５ and March ２０１６. For those patients, we have retrospectively investigated the actual medical services provided by our hospital and cooperation with other hospitals and clinics. The card was issued for ３８ patients who were under palliative treatment. More than half of all the patients issued with the card were in the department of surgery, and eight of them were colon cancer patients, who consisted the largest portion. Exacerbation of pain was the most common reason for those who used the card at the emergency outpatient visit, but more than half of them could return home within the same day. Although seven of fourteen patients who were determined to be under home care were eventually re-hospitalized, all the fourteen patients were able to spend as long time as possible at their own home due to the card system. During one year after the introduction of the OCM card system, there has been no trouble with the acceptance of emergency outpatient visits and the cooperation of Tokushima Municipal Hospital with other hospitals and clinics