Evaluating Chemical Seed Treatments for Fusarium Root Rot Control in Dry Beans and Field Peas

This study evaluated commercially available seed treatment products for their ability to control Fusarium solani and F. avenaceum, causal agents of root rot in dry edible bean and field peas, respectively, through in-vitro, growth chamber and field trials. Disease severity was assessed using a 0 to 5 scale, and root health parameters were recorded. The in-vitro tests conducted were not considered good predictors of fungicide performance in growth chamber or field trials in case of dry beans for management of F. solani. In case of field peas, in-vitro and growth chamber studies provided consistent results and allowed the identification of fludioxonil, trifloxystrobin and pyraclostrobin as the most effective products to manage F. avenaceum. Overall, integration of chemical seed treatments along with cultural practices; crop varieties partially resistant to root rot, and drench application is necessary to effectively manage Fusarium root rot of dry beans and field peas in field conditions

Digital contrast sensitivity chart with varying visual acuity: Development and validation

BACKGROUND: Contrast sensitivity (CS) evaluation is very much necessary for the diagnosis and management of eye conditions. Several disadvantages seen in the traditionally used Pelli-Robson (PR) chart persuaded us to develop a novel digital device for the evaluation of CS at various levels of visual acuity (VA) for early detection of deteriorating vision. MATERIALS AND METHODS: The new chart was constructed on the Debian software. Letters were constructed at each level of VA from 6/60 to 6/6 with a contrast of 0–2.25 logCS. The newly constructed chart was validated against the PR chart. The data were analyzed on the SPSS software version 23. The Pearson's correlation test was used to find out the correlation between the digital CS chart (DCSC) and PR charts. RESULTS: On successful construction of a DCSC with varying VA, 56 participants were examined with a mean age of 20.43 ± 1.44 years. The mean CS with PR was 1.84 ± 0.13 logCS, whereas DCSC was 2.09 ± 0.14 logCS. The correlation between the two charts was moderate having r = 0.489 with P = 0 (confidence interval 95%). CONCLUSION: A newly developed CS chart is efficient in examining the CS in clinical practice and has good repeatability; it may help in the early detection and monitoring of ocular diseases

The impact of COVID-19 on compliance to amblyopia treatment in a tertiary eye care center

Purpose: To investigate the impact of the coronavirus disease 2019 (COVID-19) outbreak on compliance to amblyopia therapy from the perspective of parents of amblyopic children. Methods: The participants for the qualitative study were enrolled from the medical records of a tertiary eye care center during the COVID-19 pandemic. The trained researcher conducted telephonic interviews consisting of validated 15 open-ended questions for 15 min. The questions were related to patients' compliance toward amblyopia treatment and the date of follow-up with their practitioners. The data collected were entered on Excel sheets in the participant's own words and later converted to a transcript for analysis. Results: A total of 217 parents of children with amblyopia who were due for follow-up were contacted through telephone. The response rate for willingness to participate was only 36% (n = 78). Of these, 76% (n = 59) of parents reported that their child was compliant toward the treatment during the therapy period and 69% reported that their child was currently not on treatment for amblyopia. Conclusion: In the current study, we have observed that although reported compliance by parents during the therapy period was good, around 69% of patients had discontinued amblyopia therapy. The main reason for the discontinuation of therapy was the failure to visit the hospital during the scheduled follow-up with the eye care practitioner

Polyamine Induction in Postharvest Banana Fruits in Response to NO Donor SNP Occurs via l-Arginine Mediated Pathway and Not via Competitive Diversion of S-Adenosyl-l-Methionine

Nitric oxide (NO) is known to antagonize ethylene by various mechanisms; one of such mechanisms is reducing ethylene levels by competitive action on S-adenosyl-L-methionine (SAM)—a common precursor for both ethylene and polyamines (PAs) biosynthesis. In order to investigate whether this mechanism of SAM pool diversion by NO occur towards PAs biosynthesis in banana, we studied the effect of NO on alterations in the levels of PAs, which in turn modulate ethylene levels during ripening. In response to NO donor sodium nitroprusside (SNP) treatment, all three major PAs viz. putrescine, spermidine and spermine were induced in control as well as ethylene pre-treated banana fruits. However, the gene expression studies in two popular banana varieties of diverse genomes, Nanjanagudu rasabale (NR; AAB genome) and Cavendish (CAV; AAA genome) revealed the downregulation of SAM decarboxylase, an intermediate gene involved in ethylene and PA pathway after the fifth day of NO donor SNP treatment, suggesting that ethylene and PA pathways do not compete for SAM. Interestingly, arginine decarboxylase belonging to arginine-mediated route of PA biosynthesis was upregulated several folds in response to the SNP treatment. These observations revealed that NO induces PAs via l-arginine-mediated route and not via diversion of SAM pool