2 research outputs found
Induction of In Vitro Resistance to Penicillin in Viridans Group Streptococci and Its Effect on Susceptibility Pattern of Other Antimicrobial Agents
Background: The development of resistance to
penicillin in Viridans Group Streptococci (VGS)
during therapy has been reported. However, the in vitro
development of resistance to penicillin or other
antimicrobial agents in VGS is mostly overlooked and
rarely reported. Aim & Objectives: To induce in vitro
resistance to penicillin in VGS and to study its effect on
susceptibility pattern of other structurally related
(Beta–lactams) and unrelated antimicrobial agents.
Material and Methods: Four isolates of VGS
susceptible to all antimicrobial agents were
manipulated in vitro to induce resistance to penicillin
by sequential exposure to increasing concentrations of
penicillin. Results: Increase in MIC values of
penicillin from 0.06-0.12 µg/ml to 2-32 µg/ml was
observed indicating development of resistance to
penicillin. A significant increase in Minimum
Inhibitory Concentration (MIC) values of ampicillin
and slight increase in MIC values of other
antimicrobial agents in some isolates was also noted.
Conclusions: Exposure to increasing concentrations of
penicillin can promote the development of resistance
to penicillin and cross-resistance to other
antimicrobial agents suggesting its mutagenic role
Stimulus-triggered Fate Conversion of Somatic Cells into Pluripotency in Chronic Wounds in Human Beings?
Bone-marrow derived stem cells are multi potential
or totipotent and are able to differentiate into numerous
cell types. Their application is indicated in various
reconstructive and restorative surgeries for rapid
healing. A technique for creating cells that have the
embryonic ability to turn into almost any cell type in
the mammalian body has been reported. Recently, an
unexpected phenomenon of somatic cell
reprogramming into pluripotent cells by exposing to
sublethal stimuli such as citrate based acidic medium
has been reported. With the concept of creating acidic
environment in chronic infected wounds to make a
condition unsuitable for growth and multiplication of
bacteria using 3% citric acid has been reported. It
would be interesting to study whether the phenomenon
of pluripotency takes place in chronic infected wounds
in human beings following the application of 3% citric
acid and plays an important role in formation of
healthy granulation tissue