Five dimensional O(N)O(N)-symmetric CFTs from conformal bootstrap

We investigate the conformal bootstrap approach to $O(N)$ symmetric CFTs in five dimension with particular emphasis on the lower bound on the current central charge. The bound has a local minimum for all $N>1$, and in the large $N$ limit we propose that the minimum is saturated by the critical $O(N)$ vector model at the UV fixed point, the existence of which has been recently argued by Fei, Giombi, and Klebanov. The location of the minimum is generically different from the minimum of the lower bound of the energy-momentum tensor central charge when it exists for smaller $N$. To better understand the situation, we examine the lower bounds of the current central charge of $O(N)$ symmetric CFTs in three dimension to compare. We find the similar agreement in the large $N$ limit but the discrepancy for smaller $N$ with the other sectors of the conformal bootstrap.Comment: 5 pages, 6 figures. v2: minor change

How should Social Studies Lessons collaborate with Museum Curators?: A Case Study of Curator’s Perceptions for School-museum Partnership in Japan

This study reports the results of an empirical investigation of Japanese museum curators’ perceptions of the role of museums in the school-museum relationship. Findings are based on data collected through semi-structured interviews with seven museum curators located in Aichi and Gifu prefectures and analyzed using M-GTA (Kinoshita, 2003; 2007; 2016). Interviews with seven curators indicate that perceptions of museums’ role were formed not only by their relationship with the schools they worked with but also by the interconnectedness of the curators’ individual work experiences and their responsibility to the expectations of the local community, including the government and visitors. These findings suggest that curators confront collections based on foundations while coordinating with relationships with schools and considering local community demands. Curator recognizes museums’ role as a social space through interactions with schools and communities. In this case study, we observed a museum that faced difficulties in its relationship with the local community, including schools. Therefore, conceptualizing curators as “creators” in social studies classes may help schools and museums establish a closer relationship

Revival of apoptotic cells that display early-stage dynamic membrane blebbing

AbstractThe critical point at which apoptosis becomes irreversible and how cells attain an anti-apoptotic state remain unknown. Here, we report that apoptotic cells undergoing early-stage dynamic membrane blebbing revive. We examined this phenomenon in cell lines that stably express 2DED2DD, a modified FADD produced by fusing the tandem death effector domains (DEDs) and tandem death domains (DDs). Induction of apoptosis caused rapid blebbing. Eight hours later, most cells shrunk while some detached from the flask. Twenty-four hours later, when activated caspase 3 decreased, more than half the cells revived and appeared normal, probably due to the induction of unidentified anti-apoptotic proteins

ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus.

Small GTPases of the ADP-ribosylation factor (ARF) family, except for ARF6, mainly localize to the Golgi apparatus, where they trigger formation of coated carrier vesicles. We recently showed that class I ARFs (ARF1 and ARF3) localize to recycling endosomes, as well as to the Golgi, and are redundantly required for recycling of endocytosed transferrin. On the other hand, the roles of class II ARFs (ARF4 and ARF5) are not yet fully understood, and the complementary or overlapping functions of class I and class II ARFs have been poorly characterized. In this study, we find that simultaneous depletion of ARF1 and ARF4 induces extensive tubulation of recycling endosomes. Moreover, the depletion of ARF1 and ARF4 inhibits retrograde transport of TGN38 and mannose-6-phosphate receptor from early/recycling endosomes to the trans-Golgi network (TGN) but does not affect the endocytic/recycling pathway of transferrin receptor or inhibit retrograde transport of CD4-furin from late endosomes to the TGN. These observations indicate that the ARF1+ARF4 and ARF1+ARF3 pairs are both required for integrity of recycling endosomes but are involved in distinct transport pathways: the former pair regulates retrograde transport from endosomes to the TGN, whereas the latter is required for the transferrin recycling pathway from endosomes to the plasma membrane

Phospholipid‐flipping activity of P4‐ATPase drives membrane curvature

リン脂質の移動によって細胞膜が変形するメカニズムを解明 --ウイルス・細菌の細胞への侵入を制御の可能性--. 京都大学プレスリリース. 2018-03-30.P4-ATPases are phospholipid flippases that translocate phospholipids from the exoplasmic/luminal to the cytoplasmic leaflet of biological membranes. All P4-ATPases in yeast and some in other organisms are required for membrane trafficking; therefore, changes in the transbilayer lipid composition induced by flippases are thought to be crucial for membrane deformation. However, it is poorly understood whether the phospholipid-flipping activity of P4-ATPases can promote membrane deformation. In this study, we assessed membrane deformation induced by flippase activity via monitoring the extent of membrane tubulation using a system that allows inducible recruitment of Bin/amphiphysin/Rvs (BAR) domains to the plasma membrane (PM). Enhanced phosphatidylcholine-flippase activity at the PM due to expression of ATP10A, a member of the P4-ATPase family, promoted membrane tubulation upon recruitment of BAR domains to the PM. This is the important evidence that changes in the transbilayer lipid composition induced by P4-ATPases can deform biological membranes

The N- or C-terminal cytoplasmic regions of P4-ATPases determine their cellular localization

Mammalian P4-ATPases specifically localize to the plasma membrane and the membranes of intracellular compartments. P4-ATPases contain 10 transmembrane domains, and their N- and C-terminal (NT and CT) regions face the cytoplasm. Among the ATP10 and ATP11 proteins of P4-ATPases, ATP10A, ATP10D, ATP11A, and ATP11C localize to the plasma membrane, while ATP10B and ATP11B localize to late endosomes and early/recycling endosomes, respectively. We previously showed that the NT region of ATP9B is critical for its localization to the Golgi apparatus, while the CT regions of ATP11C isoforms are critical for Ca2+-dependent endocytosis or polarized localization at the plasma membrane. Here, we conducted a comprehensive analysis of chimeric proteins and found that the NT region of ATP10 proteins and the CT region of ATP11 proteins are responsible for their specific subcellular localization. Importantly, the ATP10B NT and the ATP11B CT regions were found to harbor a trafficking and/or targeting signal that allows these P4-ATPases to localize to late endosomes and early/recycling endosomes, respectively. Moreover, dileucine residues in the NT region of ATP10B were required for its trafficking to endosomal compartments. These results suggest that the NT and CT sequences of P4-ATPases play a key role in their intracellular trafficking