Integrating nuclide specific and dose rate based methods for airborne and ground based gamma spectrometry

Results of joint airborne survey work conducted by SUERC and JAEA are presented, for areas to the north and south of Fukushima Daiichi using four different airborne survey systems, cross calibrated at reference sites in Scotland and near Namie. Airborne measurements were made at a series of different survey heights using three high volume NaI based spectrometers, and for the first time using a high resolution system based on the Ortec IDM HPGe spectrometer. The JAEA data sets were analysed using the same methods applied to national scale mapping in Japan since the accident. The SUERC data sets were analysed using nuclide specific approaches validated in the European ECCOMAGS project. The data presented on a digital terrain model show marked correspondence with landscape features, which both suggest the initial deposition processes, and indicate trajectories for future re-deposition by natural processes. All data sets are traceable to each other, and to the ground based calibration sites. Nuclide specific inventories have been defined, which can serve as a future reference to evaluate environmental change

Bending fatigue strength design method for machine components with complex geometries

The purpose of this study is to devise a bending fatigue strength evaluation method that can be widely used for any machine component under the consideration that bending fatigue breakage depends on the initiation of a surface fatigue crack, namely, the conditions for the initiation of a fatigue crack can be used as the criteria for bending fatigue strength design. The specially designed and manufactured three-point bending specimens with crowned round notches are used in bending fatigue experiments. The obtained bending fatigue strengths are expressed as the maximum actual stress at the critical point, which increases with decreasing notch radius. Considering the effects of the stress distribution on the bending fatigue strength, we present a method for estimating the tension fatigue strength of a smooth specimen, which is shape-independent fatigue strength and can be applied to bending fatigue strength design for machine components with complex geometries

Chitinase 3-Like 1 Promotes Macrophage Recruitment and Angiogenesis in Colorectal Cancer

Chitinase 3-like 1 (CHI3L1), one of mammalian members of the chitinase family, is expressed in several types of human cancer, and elevated serum level of CHI3L1 is suggested to be a biomarker of poor prognosis in advanced cancer patients. However, the overall biological function of CHI3L1 in human cancers still remains unknown. Studies were performed to characterize the role of CHI3L1 in cancer pathophysiology utilizing human colorectal cancer samples and human cell lines. Plasma protein and tissue mRNA expression levels of CHI3L1 in colorectal cancer were strongly upregulated. Immunohistochemical analysis showed that CHI3L1 was expressed in cancer cells and CHI3L1 expression had a significant association with the number of infiltrated macrophages and microvessel density. By utilizing trans-well migration and tube formation assays, overexpression of CHI3L1 in SW480 cells (human colon cancer cells) enhanced the migration of THP-1 cells (human macrophage cells) and HUVECs (human endothelial cells), and the tube formation of HUVECs. The knockdown of CHI3L1 by RNA interference or the neutralization of CHI3L1 by anti-CHI3L1 antibody displayed strong suppression of CHI3L1-induced migration and tube formation. Cell proliferation assay showed that CHI3L1 overexpression significantly enhanced the proliferation of SW480 cells. ELISA analysis showed that CHI3L1 increased the secretion of inflammatory chemokines, IL-8 and MCP-1, from SW480 cells through mitogen-activated protein kinase (MAPK) signaling pathway. Both neutralization of IL-8 or MCP-1 and inhibition or knockdown of MAPK in SW480 cells significantly inhibited CHI3L1-induced migration and tube formation. In a xenograft mouse model, overexpression of CHI3L1 in HCT116 cells (human colon cancer cells) enhanced the tumor growth as well as macrophage infiltration and microvessel density. In conclusion, CHI3L1 expressed in colon cancer cells promotes cancer cell proliferation, macrophage recruitment and angiogenesis. Thus, the inhibition of CHI3L1 activity may be a novel therapeutic strategy for human colorectal cancer

動物モデルにおける骨髄間質細胞シートの乱軸型皮弁の延長効果

BACKGROUND: Bone marrow stromal cells can be applied therapeutically to enhance angiogenesis; however, the use of bone marrow stromal cell suspensions reduces efficiency because of low-level attachment. The authors hypothesized that bone marrow stromal cell sheets would facilitate cell fixation, thus enhancing angiogenesis. The authors investigated flap survival area and enhancement of angiogenic factors in a rat random-pattern skin flap model after application of bone marrow stromal cell sheets. METHODS: Bone marrow stromal cell sheets (prepared from 7-week-old rat femurs) were cultured under four different hypoxic conditions. Sheets with the highest angiogenic potential, determined by an in vitro pilot study, were injected into subcutaneous layers of the rat dorsum (bone marrow stromal cell sheet group). A control group (phosphate-buffered saline only) was included. On day 2 after injection, caudally based random-pattern skin flaps (12 × 3 cm) were elevated. On day 7 after elevation, surviving skin flap areas were measured. Skin samples were harvested from each flap and gene expression levels of vascular endothelial growth factor and basic fibroblast growth factor were measured by quantitative real-time polymerase chain reaction. RESULTS: Skin flap survival area (71.6 ± 2.3 percent versus 51.5 ± 3.3 percent) and levels of vascular endothelial growth factor and basic fibroblast growth factor were significantly higher in the bone marrow stromal cell sheet group than in the control group (p < 0.05). CONCLUSIONS: Implantation of bone marrow stromal cell sheets increased the survival area of random-pattern skin flaps. Expression of angiogenic factors may have contributed to the increased flap survival.博士（医学）・甲第658号・平成28年11月24日Copyright © 2015 American Society of Plastic SurgeonsThe definitive version is available at " http://dx.doi.org/10.1097/PRS.0000000000001679

Estimation Procedures and TFP Analysis of the JIP Database 2006 Provisional Version

(Introduction) The purpose of this paper is to explain the preliminary version of the newly compiled Japan Industrial Productivity Database (JIP 2006) and report some results of our growth accounting analysis based on this database. The JIP 2006 contains information on 108 sectors from 1970 to 2002 that can be used for total factor productivity analyses. These sectors cover the whole Japanese economy. The JIP Database was compiled as part of the RIETI (Research Institute of Economy, Trade and Industry) research project "Study on Industry-Level and Firm-Level Productivity in Japan." The original version of the JIP Database (ESRI/Hi-Stat JIP Database 2003) was compiled in a collaboration between ESRI (Economic and Social Research Institute, Cabinet Office, Government of Japan) as part of its research project on "Japan's Potential Growth" and Hitotsubashi University as part of its Hi-Stat project (A 21st-Century COE Program, Research Unit for Statistical Analysis in the Social Sciences). The authors are grateful to ESRI and members of the Hi-Stat team for the support and cooperation provided for our present RIETI project. At this moment, the major data available are sectoral capital service input indices and labor service input indices, including information on real capital stocks and the nominal cost of capital by type of capital and by industry, the nominal and real values of sectoral gross output and intermediate input, as well as some supplementary tables, such as statistics on trade, inward and outward FDI, and Japan's industrial structure. All real values are based on 1995 prices. For growth accounting, nominal labor costs and nominal capital services for 108 industries are also estimated. The sum of these two values for each industry is not adjusted to be equal to the value added of that industry at factor cost base. The final version of the JIP 2006 is scheduled to be released by November, 2006. The final version will include nominal and real annual input-output tables, detailed information on ICT capital services and some additional statistics, such as R&D stocks and capacity utilization rates at the detailed sectoral level. For scholars familiar with the JIP 2003, we here briefly summarize the main differences between and the main similarities of the 2006 and 2003 versions of the JIP. 1. The JIP 2003 is based on the 1968 SNA, while the JIP 2006 is based on the 1993 SNA. The capital stock of the JIP 2006 includes order-made software, plant engineering, and assets accumulated by the search for minerals. The JIP 2003 uses SNA statistics as control totals. Following Japan's present SNA statistics, capital stock in the preliminary version of the JIP 2006 does not include prepackaged and in-house software. However, the final version of the JIP 2006 will include two sets of statistics, one in which capital stock does not include prepackaged and in-house software and one in which it does. 2. In the case of the JIP 2006, labor input data include detailed information on labor input cross-classified by categories of labor. The paper is organized as follows: In the next section, we report the estimation procedures of our annual input-output tables. In Sections 2 and 3, we explain the capital service input data and the labor input data of the JIP 2006, respectively. Finally, in Section 4, we analyze Japan's sectoral and macro TFP growth.

Ultraviolet Action Spectrum and Effect of EPC-K1 on Ultraviolet Radiation-induced Injury in Cultured Normal Human Epidermal Keratinocytes

This study was aimed to determine the ultraviolet (UV: 235-310nm) action spectrum for killing normal human epidermal keratinocytes (NHEK) and to investigate the preventive effect of EPC-K1, a phosphate diester of vitamin C and vitamin E on UV radiation-induced NHEK injury. NHEK were cultured in EpiLife medium supplemented with Human Keratinocyte Growth Supplement Kit. NHEK viability was determined by crystal violet (CV) staining 48 h after the UV irradiation. The mRNA expressions of the C/EBP homologous protein (Chop) transcription factor and endoplasmic reticulum-resident molecular chaperone, Bip, were determined by RT-PCR analyses. UV was especially effective in killing NHEK when applied in the wavelength region of 250-280nm. The minimum exposure dose required to kill 50% of cells (LD50) was 1.64mJ/cm2 at 269nm. At 235 and 310nm, the LD50 for NHEK was 6.62 and 293mJ/cm2, respectively. Irradiation of 660-mJ/cm2 at 310nm significantly decreased the cell viability to 30% of control (without irradiation). The addition of 0.1mM EPC-K1 after irradiation returned the cell viability to 118%. Six hours after the 660-mJ/cm2 irradiation at 310nm, Chop and Bip mRNA levels in NHEK were increased to 487% and 283%, respectively, and were not significantly affected by EPC-K1. Chop and Bip are responsive to ER stress. These results suggested that EPC-K1 exerts a protective effect against UV-induced NHEK injury, and further studies should investigate the molecular mechanism underlying this effect