Antimicrobial Activity and Germination Conditions of the Medicinal Plant Argemone mexicana

Commonly called the Mexican prickly poppy, Argemone mexicana is a stress-resistant member of the Papaveraceae family of plants that has been used in traditional medicine for centuries by indigenous communities in Mexico and Western parts of the United States. This plant has been used to treat a wide variety of ailments, including skin diseases and intestinal infections, with reported antimicrobial properties. However, these properties are poorly understood and no bioactive compounds have yet been identified in the plant to account for this antimicrobial action. Herein, we describe the growth conditions and preliminarily characterize the antibiotic effects of different parts of the A. mexicana plant. We report that 2 mg of A. mexicana methanol root extract possesses antibacterial activity against the bacteria Bacillus cereus and Staphylococcus aureus, while the same concentration has no inhibitory effect on the fungus Candida albicans. Moreover, the methanol root fraction displays a stronger antibacterial effect, when compared to either the methanol seed or leaf fractions at the same concentrations and normalized to background solvent alone. Additionally, we show that when supplemented with 1000 mg/L of the phytohormone gibberellic acid (GA), germination rates of A. mexicana are significantly increased when compared to germination with either no GA or 100 mg/L GA. These preliminary results warrant further research into defining the antimicrobial properties and chemicals produced in the roots of these plants and are especially significant given the growing global concern of antibiotic-resistant ‘superbugs’ and lack of new antimicrobial drug discovery

Antimicrobial Activity and Germination Conditions of the Medicinal Plant Argemone mexicana

Commonly called the Mexican prickly poppy, Argemone mexicana is a stress-resistant member of the Papaveraceae family of plants that has been used in traditional medicine for centuries by indigenous communities in Mexico and Western parts of the United States. This plant has been used to treat a wide variety of ailments, including skin diseases and intestinal infections, with reported antimicrobial properties. However, these properties are poorly understood and no bioactive compounds have yet been identified in the plant to account for this antimicrobial action. Herein, we describe the growth conditions and preliminarily characterize the antibiotic effects of different parts of the A. mexicana plant. We report that 2 mg of A. mexicana methanol root extract possesses antibacterial activity against the bacteria Bacillus cereus and Staphylococcus aureus, while the same concentration has no inhibitory effect on the fungus Candida albicans. Moreover, the methanol root fraction displays a stronger antibacterial effect, when compared to either the methanol seed or leaf fractions at the same concentrations and normalized to background solvent alone. Additionally, we show that when supplemented with 1000 mg/L of the phytohormone gibberellic acid (GA), germination rates of A. mexicana are significantly increased when compared to germination with either no GA or 100 mg/L GA. These preliminary results warrant further research into defining the antimicrobial properties and chemicals produced in the roots of these plants and are especially significant given the growing global concern of antibiotic-resistant ‘superbugs’ and lack of new antimicrobial drug discovery

Characterizing the Antimicrobial and Anticancer Activities and Several Associated Bioactive Compounds of Argemone mexicana

Commonly called the Mexican prickly poppy, Argemone mexicana is a stress-resistant member of the Papaveraceae family of plants that has been used in traditional medicine for centuries by indigenous communities in Mexico and Western parts of the United States. This plant has been used to treat a wide variety of ailments, including skin diseases and intestinal infections, with reported antimicrobial and anticancer properties. However, these properties are poorly understood, with few associated bioactive compounds yet identified. Herein, we describe the germination conditions of A. mexicana and preliminarily characterize the antimicrobial and anticancer activities of different parts of the plant. We show that when comparing 1 mg of each sample normalized to background solvent alone, the A. mexicana methanol outer root and leaf extracts possess the strongest antimicrobial activity, with greatest effects against the gram-positive bacteria tested, and less activity against the gram-negative bacteria and fungi tested. Additionally, we report that when using the MTT colorimetric assay, the outer root and leaf methanol extracts and the seed hexane extract have pronounced inhibitory effects against T84 human colon cancer cells. Using normal-phase column chromatography and subsequent mass spectrometry analysis of the outer root and leaf methanol fractions, we have begun to chemically characterize several candidate antibacterial compounds. These preliminary results warrant further research into defining the bioactive chemicals produced in the roots, leaves and seeds of A. mexicana and are especially significant given the growing global concern of antibiotic-resistant ‘superbugs’ and lack of new antimicrobial and anticancer drug discovery

Characterizing the Antimicrobial and Anticancer Activities and Several Associated Bioactive Compounds of Argemone mexicana

Commonly called the Mexican prickly poppy, Argemone mexicana is a stress-resistant member of the Papaveraceae family of plants that has been used in traditional medicine for centuries by indigenous communities in Mexico and Western parts of the United States. This plant has been used to treat a wide variety of ailments, including skin diseases and intestinal infections, with reported antimicrobial and anticancer properties. However, these properties are poorly understood, with few associated bioactive compounds yet identified. Herein, we describe the germination conditions of A. mexicana and preliminarily characterize the antimicrobial and anticancer activities of different parts of the plant. We show that when comparing 1 mg of each sample normalized to background solvent alone, the A. mexicana methanol outer root and leaf extracts possess the strongest antimicrobial activity, with greatest effects against the gram-positive bacteria tested, and less activity against the gram-negative bacteria and fungi tested. Additionally, we report that when using the MTT colorimetric assay, the outer root and leaf methanol extracts and the seed hexane extract have pronounced inhibitory effects against T84 human colon cancer cells. Using normal-phase column chromatography and subsequent mass spectrometry analysis of the outer root and leaf methanol fractions, we have begun to chemically characterize several candidate antibacterial compounds. These preliminary results warrant further research into defining the bioactive chemicals produced in the roots, leaves and seeds of A. mexicana and are especially significant given the growing global concern of antibiotic-resistant ‘superbugs’ and lack of new antimicrobial and anticancer drug discovery

Characterizing the Antimicrobial and Anticancer Activities and Several Associated Bioactive Compounds of Argemone mexicana

Commonly called the Mexican prickly poppy, Argemone mexicana is a stress-resistant member of the Papaveraceae family of plants that has been used in traditional medicine for centuries by indigenous communities in Mexico and Western parts of the USA. This plant has been used to treat a wide variety of ailments, including skin diseases and intestinal infections, with reported antimicrobial and anticancer properties. However, these properties are poorly understood, with no associated bioactive compounds yet identified. Herein, we describe the germination conditions of A. mexicana and preliminarily characterize the antimicrobial and anticancer activities of different parts (seeds, leaves, inner vs. outer roots) of the plant. We show that when comparing 1 mg of each sample normalized to background solvent alone, the A. mexicana methanol outer root and leaf extracts possess the strongest antimicrobial activity, with greatest effects against gram-positive bacteria tested, and less activity against gram-negative bacteria and fungi tested. Additionally, we report that when using the MTT colorimetric assay, the outer root and leaf methanol extracts and the seed hexane extract have pronounced inhibitory effects against T84 human colon cancer cells. Using normal-phase column chromatography and subsequent mass spectrometry analysis of the outer root and leaf methanol fractions, we have begun to chemically characterize several candidate antibacterial compounds. These preliminary results warrant further research into defining the bioactive chemicals produced in the roots, leaves and seeds of A. mexicana and are especially significant given the growing global concern of antibiotic-resistant ‘superbugs’ and lack of new antimicrobial and anticancer drug discovery

Characterizing the Antimicrobial and Anticancer Activities and Several Associated Bioactive Compounds of Argemone mexicana

Commonly called the Mexican prickly poppy, Argemone mexicana is a stress-resistant member of the Papaveraceae family of plants that has been used in traditional medicine for centuries by indigenous communities in Mexico and Western parts of the USA. This plant has been used to treat a wide variety of ailments, including skin diseases and intestinal infections, with reported antimicrobial and anticancer properties. However, these properties are poorly understood, with no associated bioactive compounds yet identified. Herein, we describe the germination conditions of A. mexicana and preliminarily characterize the antimicrobial and anticancer activities of different parts (seeds, leaves, inner vs. outer roots) of the plant. We show that when comparing 1 mg of each sample normalized to background solvent alone, the A. mexicana methanol outer root and leaf extracts possess the strongest antimicrobial activity, with greatest effects against gram-positive bacteria tested, and less activity against gram-negative bacteria and fungi tested. Additionally, we report that when using the MTT colorimetric assay, the outer root and leaf methanol extracts and the seed hexane extract have pronounced inhibitory effects against T84 human colon cancer cells. Using normal-phase column chromatography and subsequent mass spectrometry analysis of the outer root and leaf methanol fractions, we have begun to chemically characterize several candidate antibacterial compounds. These preliminary results warrant further research into defining the bioactive chemicals produced in the roots, leaves and seeds of A. mexicana and are especially significant given the growing global concern of antibiotic-resistant ‘superbugs’ and lack of new antimicrobial and anticancer drug discovery

Antimicrobial Activity and Germination Conditions of the Medicinal Plant \u3ci\u3eArgemone mexicana\u3c/i\u3e

Commonly called the Mexican prickly poppy, Argemone mexicanais a stress-resistant member of the Papaveraceae family of plants that has been used in traditional medicine for centuries by indigenous communities in Mexico and Western parts of the United States. This plant has been used to treat a wide variety of ailments, including skin diseases and intestinal infections, with reported antimicrobial properties. However, these properties are poorly understood and no bioactive compounds have yet been identified in the plant to account for this antimicrobial action. Herein, we describe the growth conditions and preliminarily characterize the antibiotic effects of different parts of the A. mexicana plant. We report that 2 mg of A. mexicanamethanol root extract possesses antibacterial activity against the bacteria Bacillus cereusand Staphylococcus aureus, while the same concentration has no inhibitory effect on the fungus Candida albicans. Moreover, the methanol root fraction displays a stronger antibacterial effect, when compared to either the methanol seed or leaf fractions at the same concentrations and normalized to background solvent alone. Additionally, we show that when supplemented with 1000 mg/L of the phytohormone gibberellic acid (GA), germination rates of A. mexicana are significantly increased when compared to germination with either no GA or 100 mg/L GA. These preliminary results warrant further research into defining the antimicrobial properties and chemicals produced in the roots of these plants and are especially significant given the growing global concern of antibiotic-resistant ‘superbugs’ and lack of new antimicrobial drug discovery

Antimicrobial Activity and Germination Conditions of the Medicinal Plant \u3ci\u3eArgemone mexicana\u3c/i\u3e

Commonly called the Mexican prickly poppy, Argemone mexicanais a stress-resistant member of the Papaveraceae family of plants that has been used in traditional medicine for centuries by indigenous communities in Mexico and Western parts of the United States. This plant has been used to treat a wide variety of ailments, including skin diseases and intestinal infections, with reported antimicrobial properties. However, these properties are poorly understood and no bioactive compounds have yet been identified in the plant to account for this antimicrobial action. Herein, we describe the growth conditions and preliminarily characterize the antibiotic effects of different parts of the A. mexicana plant. We report that 2 mg of A. mexicanamethanol root extract possesses antibacterial activity against the bacteria Bacillus cereusand Staphylococcus aureus, while the same concentration has no inhibitory effect on the fungus Candida albicans. Moreover, the methanol root fraction displays a stronger antibacterial effect, when compared to either the methanol seed or leaf fractions at the same concentrations and normalized to background solvent alone. Additionally, we show that when supplemented with 1000 mg/L of the phytohormone gibberellic acid (GA), germination rates of A. mexicana are significantly increased when compared to germination with either no GA or 100 mg/L GA. These preliminary results warrant further research into defining the antimicrobial properties and chemicals produced in the roots of these plants and are especially significant given the growing global concern of antibiotic-resistant ‘superbugs’ and lack of new antimicrobial drug discovery

Characterizing the Antimicrobial and Anticancer Activities and Several Associated Bioactive Compounds of Argemone mexicana

Commonly called the Mexican prickly poppy, Argemone mexicana is a stress-resistant member of the Papaveraceae family of plants that has been used in traditional medicine for centuries by indigenous communities in Mexico and Western parts of the USA. This plant has been used to treat a wide variety of ailments, including skin diseases and intestinal infections, with reported antimicrobial and anticancer properties. However, these properties are poorly understood, with no associated bioactive compounds yet identified. Herein, we describe the germination conditions of A. mexicana and preliminarily characterize the antimicrobial and anticancer activities of different parts (seeds, leaves, inner vs. outer roots) of the plant. We show that when comparing 1 mg of each sample normalized to background solvent alone, the A. mexicana methanol outer root and leaf extracts possess the strongest antimicrobial activity, with greatest effects against gram-positive bacteria tested, and less activity against gram-negative bacteria and fungi tested. Additionally, we report that when using the MTT colorimetric assay, the outer root and leaf methanol extracts and the seed hexane extract have pronounced inhibitory effects against T84 human colon cancer cells. Using normal-phase column chromatography and subsequent mass spectrometry analysis of the outer root and leaf methanol fractions, we have begun to chemically characterize several candidate antibacterial compounds. These preliminary results warrant further research into defining the bioactive chemicals produced in the roots, leaves and seeds of A. mexicana and are especially significant given the growing global concern of antibiotic-resistant ‘superbugs’ and lack of new antimicrobial and anticancer drug discovery

Characterizing the Antimicrobial and Anticancer Activities and Several Associated Bioactive Compounds of Argemone mexicana

Commonly called the Mexican prickly poppy, Argemone mexicana is a stress-resistant member of the Papaveraceae family of plants that has been used in traditional medicine for centuries by indigenous communities in Mexico and Western parts of the USA. This plant has been used to treat a wide variety of ailments, including skin diseases and intestinal infections, with reported antimicrobial and anticancer properties. However, these properties are poorly understood, with few associated bioactive compounds yet identified. Herein, we describe the germination conditions of A. mexicana and preliminarily characterize the antimicrobial and anticancer activities of different parts (seeds, leaves, inner vs. outer roots) of the plant. We show that when comparing 1 mg of each sample normalized to background solvent alone, the A. mexicana methanol outer root and leaf extracts possess the strongest antimicrobial activity, with greatest effects against the gram-positive bacteria tested, and less activity against the gram-negative bacteria and fungi tested. Additionally, we report that when using the MTT colorimetric assay, the outer root, leaf methanol, and the seed hexane extracts have pronounced inhibitory effects against T84 human colon cancer cells. Using normal-phase column chromatography and subsequent mass spectrometry analysis of the outer root and leaf methanol fractions, we have begun to chemically characterize several candidate antibacterial compounds. These preliminary results warrant further research into defining the bioactive chemicals produced in the roots, leaves and seeds of A. mexicana and are especially significant given the growing global concern of antibiotic-resistant ‘superbugs’ and lack of new antimicrobial and anticancer drug discovery