15 research outputs found
Cardiovascular development: towards biomedical applicability: Epicardium-derived cells in cardiogenesis and cardiac regeneration
During cardiogenesis, the epicardium grows from the proepicardial organ to form the outermost layer of the early heart. Part of the epicardium undergoes epithelial-mesenchymal transformation, and migrates into the myocardium. These epicardium- derived cells differentiate into interstitial fibroblasts, coronary smooth muscle cells, and perivascular fibroblasts. Moreover, epicardium-derived cells are important regulators of formation of the compact myocardium, the coronary vasculature, and the Purkinje fiber network, thus being essential for proper cardiac development. The fibrous structures of the heart such as the fibrous heart skeleton and the semilunar and atrioventricular valves also depend on a contribution of these cells during development. We hypothesise that the essential properties of epicardium-derived cells can be recapitulated in adult diseased myocardium. These cells can therefore be considered as a novel source of adult stem cells useful in clinical cardiac regeneration therapy
An ultracytochemical study on the dynamics of alkaline phosphatase-positive granules in rat neutrophils
Alkaline phosphatase (ALPase) activity was
examined by cerium-based uitracytochemistry in
isolated rat neutrophils following stimulation with
phorbol myristate acetate (PMA) or N-formylmethionyl-leucyl-phenylalanine (fMLP). In control
neutrophils, low levels of ALPase activity were detected
in small tubular and spherical compartments distributed
throughout the cytoplasm. Neutrophils stimulated for
2.5, 5, 15, and 30 min with 50 nglml PMA or 10-7 M
fMLP displayed a time-dependent increase in ALPase
activity. At 2.5 min, an increase in activity was first
identified in compartments that were aggregated in the
central regions of the cell. By 15 min, a dense precipitate
was seen in tubular or elongated bead-like structures that
extended to and made contact with the plasma
membrane. Large enzyme-positive vacuoles were also
observed in regions near the plasma membrane. At the
longer stimulation times, a fine precipitate was present
on the cell surface of the neutrophil in regions where
subplasmalemmal ALPase activity was present. The
results of this study indicate that an increase in activity
and a redistribution of ALPase-positive structures occurs
in neutrophils in response to stimulation with PMA and
fMLP. It is likely that these compartments are latent
pools of ALPase which, upon stimulation, fuse and
mobilize the enzyme activity to the cell surface