Cohesion policy funding for innovation and the knowledge economy

This paper examines roles of cohesion policy in financing support for research and development and technological innovation

Image_1_T Cell Receptor–Major Histocompatibility Complex Interaction Strength Defines Trafficking and CD103+ Memory Status of CD8 T Cells in the Brain.PDF

<p>T cell receptor–major histocompatibility complex (TCR–MHC) affinities span a wide range in a polyclonal T cell response, yet it is undefined how affinity shapes long-term properties of CD8 T cells during chronic infection with persistent antigen. Here, we investigate how the affinity of the TCR–MHC interaction shapes the phenotype of memory CD8 T cells in the chronically Toxoplasma gondii-infected brain. We employed CD8 T cells from three lines of transnuclear (TN) mice that harbor in their endogenous loci different T cell receptors specific for the same Toxoplasma antigenic epitope ROP7. The three TN CD8 T cell clones span a wide range of affinities to MHCI–ROP7. These three CD8 T cell clones have a distinct and fixed hierarchy in terms of effector function in response to the antigen measured as proliferation capacity, trafficking, T cell maintenance, and memory formation. In particular, the T cell clone of lowest affinity does not home to the brain. The two higher affinity T cell clones show differences in establishing resident-like memory populations (CD103⁺) in the brain with the higher affinity clone persisting longer in the host during chronic infection. Transcriptional profiling of naïve and activated ROP7-specific CD8 T cells revealed that Klf2 encoding a transcription factor that is known to be a negative marker for T cell trafficking is upregulated in the activated lowest affinity ROP7 clone. Our data thus suggest that TCR–MHC affinity dictates memory CD8 T cell fate at the site of infection.</p

Supplementary Table 1. from Peripheral self-reactivity regulates antigen-specific CD8 T-cell responses and cell division under physiological conditions

The transcriptional profile of Rop7-III compared to that of Rop7-II. 115 genes were identified to be significantly expressed with at least a two-fold difference

Supplementary Table 2. from Peripheral self-reactivity regulates antigen-specific CD8 T-cell responses and cell division under physiological conditions

<b>Gene set enrichment analysis of Rop7-II versus Rop7-III.</b> GeneGo Metacore Biological processes found to be enriched (FDR≥0.01) with genes that were significantly differentially expressed between Rop7-II and Rop7-III CD8 T cells