21 research outputs found
Studies on genetic relationships and diversity in arecanut (Areca catechu L.) germplasm utilizing RAPD markers
Get PDFIn the present investigation, genetic relationship among 60 arecanut germplasm, consisting of both indigenous and exotic accessions, were assessed using 14 polymorphic RAPD primers. The average polymorphism was 6.64 markers per primer. The PIC values among the 14 primers ranged from 0.19 to 0.49. Similarity values among the accessions ranged between 0.68 and 0.93. Cluster analysis revealed two major clusters. The Indian collections Konkan I, Konkan II and Maidhan formed a separate cluster. All the collections from Indonesia, Sri Lanka, Vietnam, Fiji, Solomon Islands, Singapore and China and some Indian collections (viz., Andaman and Nicobar Islands and North East germplasm collections) formed a second cluster. The clustering pattern was, in general, in accordance with the geographical origin of the collections. The results obtained from this study are crucial for developing effective management strategies for genetic improvement of arecanut
Genetic Diversity Studies and Screening for Fusarium Wilt (Fusarium udum Butler) Resistance in Wild Pigeonpea Accessions, Cajanus scarabaeoides (L.) Thouars
No full textNot AvailableWild relatives play an important role in the genetic improvement of cultivated crops. Cajanus scarabaeoides (L.) Thouars is one of the important wild species, which possess several desirable traits. Investigations on 67 accessions of C. scarabaeoides and 3 cultivated varieties for total soluble seed protein profiles using Sodium Dodecyl Sulphate-Poly Acryl amide Gel Electrophoresis resolved the protein bands ranged from 4-11. Based on the presence or absence of bands, similarity values were calculated and dendrogram was constructed using Unweighted Pair Group Method with Arithmetic Mean analysis. The dendrogram formed two main clusters. The first main cluster consisted of three cultivated varieties and 55 accessions of C. scarabaeoides and the second main cluster comprised of remaining 12 accessions of C. scarabaeoides. Similarity percentage ranged from 38.5 to 100. ICP15728 is the lone genotype which is distinctly different from remaining genotypes formed separate sub-cluster in the second main cluster with 38.5% similarity. Thirty eight accessions of C. scarabaeoides were screened against Fusarium wilt under glass house conditions. Of the 38 accessions screened seven accessions (ICP12707, ICP15689, ICP15692, ICP15732, ICP15744, ICP15748 and ICP15754) showed resistance to Fusarium wilt. Therefore, these accessions could be utilized in breeding programmes to increase the levels of resistance to Fusarium wilt in the pigeonpea cultivars, after further testing.Not Availabl
