Ultraviolet Irradiation Induces the Accumulation of Chondroitin Sulfate, but Not Other Glycosaminoglycans, in Human Skin

Ultraviolet (UV) light alters cutaneous structure and function. Prior work has shown loss of dermal hyaluronan after UV-irradiation of human skin, yet UV exposure increases total glycosaminoglycan (GAG) content in mouse models. To more fully describe UV-induced alterations to cutaneous GAG content, we subjected human volunteers to intermediate-term (5 doses/week for 4 weeks) or single-dose UV exposure. Total dermal uronyl-containing GAGs increased substantially with each of these regimens. We found that UV exposure substantially increased dermal content of chondroitin sulfate (CS), but not hyaluronan, heparan sulfate, or dermatan sulfate. UV induced the accumulation of both the 4-sulfated (C4S) and 6-sulfated (C6S) isoforms of CS, but in distinct distributions. Next, we examined several CS proteoglycan core proteins and found a significant accumulation of dermal and endothelial serglycin, but not of decorin or versican, after UV exposure. To examine regulation in vitro, we found that UVB in combination with IL-1α, a cytokine upregulated by UV radiation, induced serglycin mRNA in cultured dermal fibroblasts, but did not induce the chondroitin sulfate synthases. Overall, our data indicate that intermediate-term and single-dose UVB exposure induces specific GAGs and proteoglycan core proteins in human skin in vivo. These molecules have important biologic functions and contribute to the cutaneous response to UV

Sclerotherapy Induced Takotsubo Syndrome

A 66-year-old female was brought to the emergency department for acute-onset left-sided chest pain. Prior to arrival, she was at an outpatient appointment with a vascular surgeon for elective sclerotherapy treatment of her lower extremity varicose veins. After receiving an IV injection of polidocanol, she developed severe chest pain with left arm and jaw numbness for the first time in her life. Upon arrival to the ED, the patient reported that her symptoms had resolved. Electrocardiogram (ECG) on presentation was significant for T-wave inversions in leads V1-V3. An initial set of cardiac enzymes showed a troponin I level of 0.62 ng/mL, which subsequently increased to 2.26 ng/mL. Her echocardiogram was significant for mild left ventricular systolic dysfunction with apical hypokinesis (ejection fraction 50%). A repeat ECG showed new T-wave inversions compared to that from the time of admission. The patient eventually agreed to cardiac catheterization, which revealed patent vessels without coronary artery disease, supporting our diagnosis of Takotsubo syndrome and what is the first reported case of likely polidocanol-induced Takotsubo syndrome in the United States

Geology, geochemistry, and some genetic discussion of the Chador-Malu iron oxide-apatite deposit, Bafq District, Central Iran

© 2015, Saudi Society for Geosciences.The Chador-Malu iron oxide-apatite system (Bafq District, Central Iran) contains the largest known iron ore deposit in Iran (pre-mining reserve of 400 Mt @ 55 % Fe), and comprises the pipe-like northern (this study) and the sill-like southern orebodies of predominantly massive ore, and a sodic-calcic alteration envelope. The geology and geochemistry of the Chador-Malu deposit demonstrates its similar characteristics to the Kiruna-type deposits. There is circumstantial evidence for rare earth elements (REE) mobilization during apatite leaching by high-temperature fluids and associated monazite nucleation. Pervasive actinolitization of the rhyolitic country rocks led to the formation of actinolite-rich metasomatic host rocks, which represent another evidence for high-temperature fluids at Chador-Malu. Hydrothermal mineralization is suggested by small iron ore veins (2–3 cm thick) and breccias cemented by iron oxides, as well as a Fe-metasomatism which overprints all types of host rock alteration. Based on REE geochemistry and spatial relationships, it is proposed that a potential source for metals and P could be late-stage Fe-P melt differentiates of the Cambrian magmatism, which is consistent with the late Fe-metasomatism of the host rocks. The proposed Fe-P melts and the mineralization would be linked by hydrothermal media through the zones of ring fracture at Chador-Malu and similar parts of the Bafq district