Pengaruh Terapi Brain Gym Terhadap Peningkatan Fungsi Kognitif Pada Lanjut Usia Di Posyandu Lanjut Usia Desa Pucangan Kartasura

The increasing age of humans, the aging process occurs which affects generative changes in humans, one of which is throwback of cognitive function status. Brain Gym is one method of motion and train the brain, which is useful to improve cognitive function, especially in the elderly. The purpose of this study was to determine the effect of Brain Gym on the level of cognitive function in posyandu lansia of Pucangan village Kartasura. This type of research is Quasi Experimental Design with Pretest-Posttest Control Group Design, The population are 286 people. The sampling method used was purposive sampling. Overall sample in this study met the inclusion criteria of 20 people. To compare the cognitive level of the respondents before and after brain exercises using the Mini Mental State Examination (MMSE) were analyzed by paired t-test. Based on the results of the study, respondents had significant cognitive improvement. Analysis of research data in the experimental group using Paired Sample t-test with a significance value (α = 0.05). With tcount -4.452; p = 0.002 <0.05) and the average value sattus cognitive function before the Brain Gym therapy at 19.00 and at 21.80 after intervention. The average level of cognitive elderly control group with tvalue -2.714; (p = 0.024 <0.05). Thus the conclusion can be made that there is an influence of Brain Gym to increase cognitive function in the elderly

Epidemiological and Evolutionary Dynamics of Influenza B Viruses in Malaysia, 2012-2014

Epidemiological and evolutionary dynamics of influenza B Victoria and Yamagata lineages remained poorly understood in the tropical Southeast Asia region, despite causing seasonal outbreaks worldwide. From 2012-2014, nasopharyngeal swab samples collected from outpatients experiencing acute upper respiratory tract infection symptoms in Kuala Lumpur, Malaysia, were screened for influenza viruses using a multiplex RT-PCR assay. Among 2,010/3,935 (51.1%) patients infected with at least one respiratory virus, 287 (14.3%) and 183 (9.1%) samples were tested positive for influenza A and B viruses, respectively. Influenza-positive cases correlate significantly with meteorological factors-total amount of rainfall, relative humidity, number of rain days, ground temperature and particulate matter (PM10). Phylogenetic reconstruction of haemagglutinin (HA) gene from 168 influenza B viruses grouped them into Yamagata Clade 3 (65, 38.7%), Yamagata Clade 2 (48, 28.6%) and Victoria Clade 1 (55, 32.7%). With neuraminidase (NA) phylogeny, 30 intra-clade (29 within Yamagata Clade 3, 1 within Victoria Clade 1) and 1 inter-clade (Yamagata Clade 2-HA/Yamagata Clade 3-NA) reassortants were identified. Study of virus temporal dynamics revealed a lineage shift from Victoria to Yamagata (2012-2013), and a clade shift from Yamagata Clade 2 to Clade 3 (2013-2014). Yamagata Clade 3 predominating in 2014 consisted of intra-clade reassortants that were closely related to a recent WHO vaccine candidate strain (B/Phuket/3073/2013), with the reassortment event occurred approximately 2 years ago based on Bayesian molecular clock estimation. Malaysian Victoria Clade 1 viruses carried H274Y substitution in the active site of neuraminidase, which confers resistance to oseltamivir. Statistical analyses on clinical and demographic data showed Yamagata-infected patients were older and more likely to experience headache while Victoria-infected patients were more likely to experience nasal congestion and sore throat. This study describes the evolution of influenza B viruses in Malaysia and highlights the importance of continuous surveillance for better vaccination policy in this region.published_or_final_versio

What influences patients&rsquo; acceptance of a blood pressure telemonitoring service in primary care? A qualitative study

Adina Abdullah,1 Su May Liew,1 Nik Sherina Hanafi,1 Chirk Jenn Ng,1 Pauline Siew Mei Lai,1 Yook Chin Chia,1 Chu Kiong Loo2 1Department of Primary Care Medicine, Faculty of Medicine, University Malaya Primary Care Research Group, University of Malaya, Kuala Lumpur, Malaysia; 2Department of Artificial Intelligence, Faculty of Computer Science and Information Technology, University of Malaya, Kuala Lumpur, Malaysia Background: Telemonitoring of home blood pressure (BP) is found to have a positive effect on BP control. Delivering a BP telemonitoring service in primary care offers primary care physicians an innovative approach toward management of their patients with hypertension. However, little is known about patients&rsquo; acceptance of such service in routine clinical care.Objective: This study aimed to explore patients&rsquo; acceptance of a BP telemonitoring service delivered in primary care based on the technology acceptance model (TAM).Methods: A qualitative study design was used. Primary care patients with uncontrolled office BP who fulfilled the inclusion criteria were enrolled into a BP telemonitoring service offered between the period August 2012 and September 2012. This service was delivered at an urban primary care clinic in Kuala Lumpur, Malaysia. Twenty patients used the BP telemonitoring service. Of these, 17 patients consented to share their views and experiences through five in-depth interviews and two focus group discussions. An interview guide was developed based on the TAM. The interviews were audio-recorded and transcribed verbatim. Thematic analysis was used for analysis.Results: Patients found the BP telemonitoring service easy to use but struggled with the perceived usefulness of doing so. They expressed confusion in making sense of the monitored home BP readings. They often thought about the implications of these readings to their hypertension management and overall health. Patients wanted more feedback from their doctors and suggested improvement to the BP telemonitoring functionalities to improve interactions. Patients cited being involved in research as the main reason for their intention to use the service. They felt that patients with limited experience with the internet and information technology, who worked out of town, or who had an outdoor hobby would not be able to benefit from such a service.Conclusion: Patients found BP telemonitoring service in primary care easy to use but needed help to interpret the meanings of monitored BP readings. Implementations of BP telemonitoring service must tackle these issues to maximize the patients&rsquo; acceptance of a BP telemonitoring service. Keywords: primary health care, qualitative research, home blood pressure monitoring, telemedicin

HUBUNGAN DUKUNGAN ORANG TUA PADA ASPEK EMOSIONAL ANAK TERHADAP TINGKAT PRESTASI SEKOLAH

Latar Belakang: Ruang lingkup keperawatan jiwa meliputi seluruh rentang kehidupan baik kesehatan jiwa, masalah psikososial maupun gangguan jiwa. Anak usia sekolah sebagai klien sehat jiwa diharapkan mampu memenuhi tugas perkembangannya sehingga dapat meraih prestasi. Pencapaian prestasi anak usia sekolah memerlukan dukungan berbagai pihak termasuk orang tua. Peran orang tua berpengaruh besar pada perkembangan mental anak. Tujuan: mengetahui hubungan antara dukungan orang tua pada aspek emosional anak terhadap tingkat prestasi sekolah.Metode: jenis penelitian menggunakan pendekatan kuantitatif dengan desain penelitian cross sectional yang dilakukan dengan satu kali tanpa ada intervensi lanjutan. Teknik sampling yang digunakan adalah total sampling dengan tetap menerapkan kriteria restriksi. Besar populasi adalah 94 anak. Jumlah sampel yang dialmbil 68 anak. Pengumpulan data dukungan orang tua pada aspek emosional anak dengan kuesioner dukungan orang tua pada aspek emosional dan data tingkat prestasi sekolah menggunakan nilai rata-rata raport anak semester ganjil (1) tahun pelajaran 2021/2022. Uji analisis menggunakan uji spearman rank.Hasil: uji spearman rank didapatkan hasil p value= 0,002

HLA-E-expressing pluripotent stem cells escape allogeneic responses and lysis by NK cells

Polymorphisms in the human leukocyte antigen (HLA) class I genes can cause the rejection of pluripotent stem cell (PSC)-derived products in allogeneic recipients. Disruption of the Beta-2 Microglobulin (B2M) gene eliminates surface expression of all class I molecules, but leaves the cells vulnerable to lysis by natural killer (NK) cells. Here we show that this 'missing-self' response can be prevented by forced expression of minimally polymorphic HLA-E molecules. We use adeno-associated virus (AAV)-mediated gene editing to knock in HLA-E genes at the B2M locus in human PSCs in a manner that confers inducible, regulated, surface expression of HLA-E single-chain dimers (fused to B2M) or trimers (fused to B2M and a peptide antigen), without surface expression of HLA-A, B or C. These HLA-engineered PSCs and their differentiated derivatives are not recognized as allogeneic by CD8+ T cells, do not bind anti-HLA antibodies and are resistant to NK-mediated lysis. Our approach provides a potential source of universal donor cells for applications where the differentiated derivatives lack HLA class II expression