Incidence and effects of Varicella Zoster Virus infection on academic activities of medical undergraduates - a five-year follow-up study from Sri Lanka

<p>Abstract</p> <p>Background</p> <p>The adult population in Sri Lanka is having high level of susceptibility for Varicella Zoster Virus (VZV) infection. Among medical undergraduates, 47% are VZV seronegative. The purpose of the present study was to determine the incidence of VZV infection in medical undergraduates in Sri Lanka, and to describe the effects of VZV infection on their academic activities.</p> <p>Methods</p> <p>A retrospective cohort of medical undergraduates' susceptible for VZV infection was selected from the University of Peradeniya, Sri Lanka. Data on the incidence of VZV infection (Chickenpox) during their undergraduate period was collected using a self-administered structured questionnaire. A second questionnaire was administered to collect data on the details of VZV infection and the impact of it on their academic activities. VZV incidence rate was calculated as the number of infections per 1,000 person years of exposure. Descriptive statistics were used to describe the impact of VZV infection on academic activities.</p> <p>Results</p> <p>Out of the 172 susceptible cohort, 153 medical undergraduates were followed up. 47 students reported VZV infection during the follow up period and 43 of them participated in the study. The cumulative incidence of VZV infection during the period of five and half years of medical training was 30.7%. Incidence density of VZV infection among medical undergraduates in this cohort was 65.1 per 1,000 person years of follow-up. A total of 377 working days were lost by 43 students due to the VZV infection, averaging 8.8 days per undergraduate. Total academic losses for the study cohort were; 205 lectures, 17 practicals, 13 dissection sessions, 11 tutorials, 124 days of clinical training and 107 days of professorial clinical appointments. According to their perception they lost 1,927 study hours due to the illness (Median 50 hours per undergraduate).</p> <p>Conclusions</p> <p>The incidence of VZV infection among Sri Lankan medical undergraduates is very high and the impact of this infection on academic activities causes severe disruption of their undergraduate life. VZV immunization for susceptible new entrant medical undergraduates is recommended.</p

Cellular Radiosensitivity: How much better do we understand it?

Purpose: Ionizing radiation exposure gives rise to a variety of lesions in DNA that result in genetic instability and potentially tumorigenesis or cell death. Radiation extends its effects on DNA by direct interaction or by radiolysis of H2O that generates free radicals or aqueous electrons capable of interacting with and causing indirect damage to DNA. While the various lesions arising in DNA after radiation exposure can contribute to the mutagenising effects of this agent, the potentially most damaging lesion is the DNA double strand break (DSB) that contributes to genome instability and/or cell death. Thus in many cases failure to recognise and/or repair this lesion determines the radiosensitivity status of the cell. DNA repair mechanisms including homologous recombination (HR) and non-homologous end-joining (NHEJ) have evolved to protect cells against DNA DSB. Mutations in proteins that constitute these repair pathways are characterised by radiosensitivity and genome instability. Defects in a number of these proteins also give rise to genetic disorders that feature not only genetic instability but also immunodeficiency, cancer predisposition, neurodegeneration and other pathologies. Conclusions: In the past fifty years our understanding of the cellular response to radiation damage has advanced enormously with insight being gained from a wide range of approaches extending from more basic early studies to the sophisticated approaches used today. In this review we discuss our current understanding of the impact of radiation on the cell and the organism gained from the array of past and present studies and attempt to provide an explanation for what it is that determines the response to radiation

Prospecting for unconventional phosphate sources in lake sediments around Eppawala phosphate deposit, Sri Lanka

Phosphate fertilizer is an important input in agriculture, in the scenario of increasing food demand with the population increase. Global rock phosphate reserves are finite and exhaustible. This leads to the search for unconventional forms of phosphate. In Sri Lankan conditions, this aspect leads to a systematic study of lake sediments for locating secondary phosphate deposition in the area around Eppawala Phosphate Deposit. A total of 41 sediment samples from lake sediments upstream and downstream of the Eppawala Phosphate Deposit was analyzed to determine the respective phosphate content. Average concentrations of P2O5, CaO and K2O in downstream lake were respectively 0.80%, 0.44% and 0.25%. In upstream lake sediments, the respective values were 0.45%, 0.26% and 0.12%. Correlation analysis of downstream sediments shows a significant positive correlation (r ≥ 0.70) between P2O5 and CaO. Environmentally hazardous elements such as Cd and As were also analyzed in each sample to assess the impact of the excessive and long term usage of fertilizers in the area. Average concentrations of Cd and As in the downstream lake were 76.2 ppb and 28.8 ppb respectively and in the upstream lakes, the values were 396.8 ppb and 845.2 ppb, respectively. Each sample was analyzed to determine the 2% citric acid solubility. Results show that 29% from available total P2O5 in the upstream lake sediments and 44% from available total P2O5 from downstream lake sediments were soluble in 2% citric acid. The study reveals that the lake sediments especially in downstream have more soluble phosphate than in the Eppawala Rock Phosphate. This leads to the fact that downstream sediments have relatively higher amount of phosphate which is more soluble than in the Eppawala Rock Phosphate. Therefore, downstream lake sediments can be used as phosphate fertilizer for short term farming. Further studies on agronomic efficiency of downstream sediments are recommended

Nearshore sediment dynamics in Sri Lanka

Coastal environment is a unique morphodynamic system for diverse changes. The present study estimated coastal morphodynamic changes and sediment transportation directions at locations of Uswetakeiyawa beach nourishment and Colombo harbour expansion project areas. In addition, heavy mineral occurrences were studied in beach sediments altogether parameters of mineralogical and geochemical compositions to identify depositional environments. Moreover, time series satellite images were used to identify seasonal coastal sediment accretion and depositional trends. Uswetakeiyawa beach nourishment project area indicates complex sand accretion or erosion, and a net reduction of overall beach widths with reference to the initial field visit. This site records bi-directional sediment transportations by longshore currents during the monsoon periods. Similarly, Colombo harbour expansion project area shows that the cumulative beach erosion has increased after the construction of the breakwater (rate = 0.7 m/year from May 2000 to April 2011 and rate = 28.2 m/year from April 2011 to June 2012). The cumulative and site-specific sand accretion and erosion patterns show clear influence with the monsoon seasonality. Beaches were wider during the stormy south-western monsoon, whereas beaches were narrower during fair weather of north-east monsoon. In contrast, ilmenite, zircon, garnet, monazite and rutile were identified as main heavy minerals in beach sediments. The heavy mineral potential of the north-eastern coast was high (average about 45-50% in the Verugal and 70-85% in the Pulmoddai deposits and 3.5-5.0% in the offshore samples from Nilaveli to Kokkilai), compared to the south-western sediments (average about 10% in onshore and 2% in offshore Gin River mouth). Observed high concentrated heavy minerals in beach and low concentrated offshore sediments suggest the panning system in the surf zone to form enriched placer deposits. The whole rock major and trace compositions of beach sediments were marked enrichment of TiO2, Fe2O3, Ce, Cr, Nb, Th and V compared to the average upper continental crust values. The analysis of prominent coastal longshore transport patterns shows that clockwise flows in the eastern and north-western coasts. In the south-western coast, transport directions were divided into two branches of anti-clockwise and clockwise. Therefore, the schematic representation of monsoon-derived longshore currents suggests that the sources of heavy minerals in the Pulmoddai and Verugal deposits may have probably influenced by clastic sediment supply in eastern India and Sri Lanka (the Mahaweli River), respectively. Heavy minerals in the western coast can probably derive from Precambrian metamorphic rocks, and supplied to coast through river systems in Sri Lanka

Chloride contamination in construction aggregates due to periodic saline water intrusion: a case study in the Kaluganga River Estuary, Sri Lanka

The Kaluganga River Estuary is one of the main sources of construction sand in Sri Lanka. Salt water intrusion along this estuary due to extensive sand mining has increased over the years. Thus, the focus of the current research is to understand the relationship between river sand mining, salt water intrusion, and the resultant effects on construction sand. Two surveys were conducted along the Kaluganga Estuary along an 11 km stretch from the river mouth at predetermined intervals to measure depth water quality profiles, and to collect sediment samples. These surveys were carried out during maximum spring tide; first in a dry period and then in a wet period, to understand hydrographic effects on the quality of river sands. Sand samples were analysed for absolute chloride content and grain size distribution. Results showed significant salt water intrusion during the dry period, averaging 2,307 μS cm−1 in surface waters throughout the surveyed 11 km stretch along with 3,818 μS cm−1 (average) in bottom waters up to 5.6 km upstream from the river mouth causing above normal chloride content in the bottom sandy sediments. The high chloride content in bottom sands was recorded up to 5.5 km from the river mouth making them unsuitable for construction purposes. However, during wet period, salt water intrusion levels in the bottom waters were insignificant (average 61 μS cm−1) and the chloride content in bottom sediments was very low. This study highlighted the requirement for regulations on river estuary sandmining for construction purposes

Geo-environmental assessment along Southern and Western coastal belt of Sri Lanka after Indian Ocean mega Tsunami.

Geo-environmental assessment after the recent mega tsunami, along the southern and western coastal belt indicates varying effects to the beach profile, mineral deposits, surface and ground water, and soil. The most significant effect was due to the beach erosion which had changed the beach profile creating deep water near the shore allowing waves to break much further inland than usual triggering further coastal erosion with the south western monsoon. Destruction of live corals, salinization of drinking water and agricultural soils, water logging in abandoned inland coral pits severely causing epidemic threats, are some of the other major geo-environmental issues along this coastal region. Considerable amount of heavy minerals (from Beruwela to Hambanthota) which are potential placer deposits of ilmanite, rutile, monazite, zircon, and garnet has been removed from the coast line and deposited further inland in a wide scatted area, thus, degrading this potential placer deposit. Immediate actions have to be undertaken to rectify most of these environmental issues

Structures of water column and sediment sub-bottom in the Tangalle Bay, Sri Lanka

Water quality parameters and local morphology are important for monitoring aquatic life, and for planning any coastal development project. So far, few studies have been carried out in Sri Lanka for identifying water column structure and subbottom lithology in coastal zones. Tangalle Bay is situated in lowlands in the intermediate zone of southern Sri Lanka. Tangalle Bay replenish freshwater by minor tributaries (e.g., Kirama Oya) and Rekawa Lagoon. Field excursion in Tangalle Bay was carried out during the northeastern monsoon which is relatively less influenced by high wave-energy compared to the southwest monsoon. In this study, water quality parameters were measured using Valeport Midas CTD instrument. In addition, sub-bottom morphology was identified using Bathy 2010PCTM CHIRP sub-bottom profiler. Pre-decided systematic sampling locations were identified for covering three cross-shore and six long-shore survey lines. Water quality parameters of cross-shore and long-shore survey lines are represented separately. In cross-shore survey lines, temperature variations show warm pool in shallow water (depth 4 km) water can probably indicate well-mixed nature, perhaps due to upwelling. Less dense water can be observed in shallow water (depth < 3 m). Density variations also indicate upwelling of dense water at a distance of 4 km. The lowest salinity was detected close to the nearshore area due to freshwater supply by tributaries. Salinity variations also indicate possible vertical zonation of upwelling beyond 4 km distance from the shore. Dissolved oxygen values are high up to a 3 km distance in surface and bottom water, due to well mixing in the nearshore area. However, dissolved oxygen values are decreased in bottom water at distance of 3-4 km from the shore. In long-shore survey lines, temperature and density variations show strong stratifications same as that of cross-shore survey lines. High salinity on the bottom and low salinity on the surface water in the nearshore area can probably indicate freshwater supply from terrestrial margins. Sub-bottom profile data indicate the attached coral reef on the bed rock. This attached coral reef creates two depositional features such as layered sedimentary features on the landward side and homogeneous sediments on the seaward side of the bay