5 research outputs found
FAS-dependent cell death in α-synuclein transgenic oligodendrocyte models of multiple system atrophy
Multiple system atrophy is a parkinsonian neurodegenerative disorder. It is cytopathologically characterized by accumulation of the protein p25α in cell bodies of oligodendrocytes followed by accumulation of aggregated α-synuclein in so-called glial cytoplasmic inclusions. p25α is a stimulator of α-synuclein aggregation, and coexpression of α-synuclein and p25α in the oligodendroglial OLN-t40-AS cell line causes α-synuclein aggregate-dependent toxicity. In this study, we investigated whether the FAS system is involved in α-synuclein aggregate dependent degeneration in oligodendrocytes and may play a role in multiple system atrophy. Using rat oligodendroglial OLN-t40-AS cells we demonstrate that the cytotoxicity caused by coexpressing α-synuclein and p25α relies on stimulation of the death domain receptor FAS and caspase-8 activation. Using primary oligodendrocytes derived from PLP-α-synuclein transgenic mice we demonstrate that they exist in a sensitized state expressing pro-apoptotic FAS receptor, which makes them sensitive to FAS ligand-mediated apoptosis. Immunoblot analysis shows an increase in FAS in brain extracts from multiple system atrophy cases. Immunohistochemical analysis demonstrated enhanced FAS expression in multiple system atrophy brains notably in oligodendrocytes harboring the earliest stages of glial cytoplasmic inclusion formation. Oligodendroglial FAS expression is an early hallmark of oligodendroglial pathology in multiple system atrophy that mechanistically may be coupled to α-synuclein dependent degeneration and thus represent a potential target for protective intervention
Psychologistsâ dilemmas in career counselling practice
In this study, we explored main dilemmas psychologists face in career
counselling in two main professional settings: employment and education. Participants
included 24 experienced Portuguese psychologists, working in employment
(n = 14) and educational (n = 10) settings. We used consensual qualitative
research to conduct and analyse semi-structured interviews. Results revealed
dilemmasâ in five domains: neutrality, assessment, dual loyalty, role boundaries, and
confidentiality, with the typical dilemma in the domain of neutrality. Differences
between groups were found in the domains of dual loyalty and role boundaries.Dans cette étude, nous avons exploré les principaux dilemmes rencontrés
par les psychologues dans le conseil en orientation dans deux milieux professionnels
centraux: le placement et lâĂ©ducation. Parmi les participants figuraient 24 psychologues
portugais expérimentés travaillant dans des contextes de placement (n = 14)
et dâĂ©ducation (n = 10). Nous avons utilisĂ© la recherche qualitative consensuelle
pour mener et analyser les entretiens semi-structurés. Les reŽsultats ont révé lé des
dilemmes dans cinq domaines: la neutralitĂ©, lâĂ©valuation, la double loyautĂ©, les
limites du rÎle, et la confidentialité, avec le dilemme typique dans le domaine de la
neutralité. Les différences entre les groupes ont été identifiees dans les domaines de
la double loyauté et les limites du rÎle.In dieser Studie untersuchten wir die hauptsÀ chlichen Dilemmata, mit denen Psychologen in der Berufsberatung in zwei wesentlichen professionellen
Einrichtungen konfrontiert sind: Beruf und Bildung. Zu den Teilnehmern geho
šrten 24 erfahrene portugiesische Psychologen, die in Einrichtungen von Beruf
(n = 14) und Bildung (n = 10) arbeiteten. Wir verwendeten einvernehmliche
qualitative Forschung um semi-strukturierte Interviews durchzufušhren und zu
analysieren. Die Ergebnisse zeigten Dilemmata in fĂŒnf Bereichen: NeutralitĂ€t,
Beurteilung, doppelte LoyalitÀt, Rollengrenzen und Vertraulichkeit, mit dem
typischen Dilemma in der DomÀne der NeutralitÀt. Unterschiede zwischen den
Gruppen wurden in den Bereichen der doppelten LoyalitÀt und Rollengrenzen
gefunden