Effects of whole-body gammagamma-irradiation on the biosynthesis of certain serum proteins. Progress report, September 1, 1972--December 31, 1973

Whole-body gamma irradiation stimulated the in vitro uptake of the model amino acid, alpha -aminoisobutyric acid (AIB) in rat liver. The Km was 5.1 and 9.2 mM for liver slices from normal and irradiated rats; Vmax increased from 10 to 25 mu moles/gm tissue/hr. When /sup 14/C-cycloleucine was used as a tracer, irradiation increased the Na/sup +/-independent component of transport. Although Na+ ions are required, no stoichiometric relationship could be demonstrated. No effect of irradiation was observed on /sup 22/Na uptake. Irradiation reduced the recovery of plasma membranes from liver homogenates. Although the membrane obtained was low in lipids, irradiation had not damaged either the ouabain-sensitive Na/sup +/, K/sup +/(Mg/sup ++)ATPase or the K/sup +- p/-nitrophenylphoaphatase. (auth