Growth of pea plants (Pisum sativum L.) subjected to different soil water potentials : physiological indexes

A ervilha é cultivada no Brasil Central, durante o inverno seco, exigindo para o pleno êxito da cultura o uso da irrigação. Assim, o presente trabalho se propõe a avaliar os efeitos do potencial da água no solo nos índices fisiológicos da análise quantitativa de crescimento de plantas de ervilha (Pisum sativum L.). O experimento foi montado em casa de vegetação, em solos de textura argilosa, com delineamento experimental inteiramente casualizado, com quatro tratamentos decorrentes de potenciais mínimos da água no solo (-33, -100, -200 e -1500 kPa) em três repetições, cada qual contendo duas plantas de ervilha, cultivar Caprice. Os resultados obtidos evidenciaram que a redução do potencial água no solo, induziu o decréscimo na área foliar, sem interferir no comportamento da razão de massa foliar, razão de área foliar, taxa assimilatória líquida e taxa de crescimento relativo. _________________________________________________________________________________________ ABSTRACT: Peas are cultivated in the central region of Brazil, during the dry winter, demanding for the complete success the use of irrigation. Therefore, the present work has the aim of evaluating soil water potential effects on the indexes of physiological growth analysis of peas (Pisum sativum L.). The experiment was carried out in a greenhouse using soils of clayey texture, in a fully randomized design, with four treatments, based on minimum soil water potentials (-33, -100, -200 and -1500 kPa) in three replicates, each one containing two pea plants Caprice cultivar. The results obtained indicate that the reduction of soil water potential induced the decrease of leaf area, but did not interfer on the behaviour of leaf weight ratio, leaf area ratio, net assimilation rate and relative growth rate

Ação inseticida do extrato de Derris amazonica Killip para Cerotoma arcuatus Olivier (Coleoptera: Chrysomelidae

A abundância e o potencial inseticida de Derris amazonica e a necessidade de controle de Cerotoma arcuatus Olivier (Coleoptera: Chrysomelidae) na cultura do feijão-caupi (Vigna unguiculata L. Walp) estimularam a realização desta pesquisa, que objetivou avaliar a ação inseticida do extrato de D. amazonica a adultos de C. arcuatus em condições de laboratório. Os bioensaios testaram as vias de intoxicação por ingestão de folhas contaminadas, contato com superfície contaminada e aplicação tópica, com delineamento experimental inteiramente casualizado, com quatro repetições. Os valores de mortalidade e consumo foliar dos insetos foram submetidos à análise de regressão, sendo utilizada a análise de Probit para determinação das CL50, da DL50 e dos TL50. O extrato de D. amazonica, contendo 3,7% de rotenona, foi tóxico para adultos de C. arcuatus via ingestão de folhas contaminadas (CL50=15,14 µL do extrato.mL-1 de água), superfície contaminada (CL50=0,45 µL do extrato.cm-2) e aplicação tópica (DL50=1,44 µL do extrato.g-1 do inseto). Mortalidades de adultos de C. arcuatus superiores a 80% e os menores tempos letais médios foram obtidos na concentração de 5% (v v-1) do extrato em todos os bioensaios. O consumo foliar de adultos de C. arcuatus foi inversamente proporcional a concentração do extrato quando expostos por via de ingestão foliar ou aplicação tópica, sendo inclusive observada inibição da alimentação dos indivíduos. O extrato de D. amazonica é tóxico para C. arcuatus e inibe a alimentação dos insetos a partir da concentração de 1% (v v-1).The abundance and insecticidal potential of Derris amazonica in addition to need of controlling Cerotoma arcuatus for bean crop stimulated this research. The objective of this work was to evaluate insecticide action of the extract of D. amazonica to adults of C. arcuatus in laboratory conditions. The bioassays were carried out using three distend methodologies: leaf intake, contact in treated surface (filter paper) and topical application. A completed randomized experimental design was used with four replications. Mortality values and leaf consumption of the insects were subjected to regression analyses, being the Probit analyses used to determine of the i.e., LC50, LT50 and LD50. The extract of D. amazonica containing 3.7% of rotenone was toxic to adults C. arcuatus when exposed to treated leaves (LC50 = 15.14 µl.mL-1), treated surface (LC50 = 0.45 µl.cm-2) and subjected to topical exposure (LD50 = 1.44 µl.g-1). In all bioassays the adults mortality was higher than 80% with lower median lethal times obtained with 5% (v.v-1) concentrations of the extract. Leaf consumption by adults C. arcuatus was inversely proportional to the concentration of the extract when exposed by leaf intake or topical application, also being observed inhibition of feeding individuals. The extract of D. amazonica is toxic to C. arcuatus and inhibits the feeding of insects from the concentration of 1% (v v-1)

Uso De Enxerto ósseo Autógeno Nas Reconstruções Da Cavidade Bucal. Análise Retrospectiva De 07 Anos

Currently, there is great demand for patients who requires treatment with bone integrable dental implants, however this situation is often hampered by the changes in the edentulous alveolar ridge, which may make the installation of the implant more complicated. The autogenous bone graft are current days the choice of treatments for patients with insufficient bone volume to receive implants and that to receive bone integrable implants. The purpose of this study was to evaluate retrospectively the use of autogenous bone graft in patients treated in the Surgery Area of Oral and Maxillofacial from the College of Dentistry of Piracicaba, by de State University of Campinas in the period July 2001 to July 2008. The results show that the intraoral donors areas were used in 95,1% of the cases, and that the maxillary tuberosity was used in 39.8% of the cases, the ascending branch in 31,4% and the mandibular symphysis in 28,8% of the cases. The "onlay" block graft was the most commonly technique employed (53,7%), followed by the particulate bone graft (36,6%) and by sinus lifting of the maxillary (9,7%). In conclusion, whenever possible we should choose intraoral donors areas, and in this work, these donors areas were the most commonly employed, and the maxillary tuberosity the most used.504221225Atwood, D.A., Reduction of residual ridges: a major oral disease entity (1971) J Prosthet Dent, 26, pp. 266-279Tallgren, A., The continuing reduction of the residual alveolar ridges in complete denture wearers: a mixed-longitudinal study covering 25 years (1972) J Prosthet Dent, 27, pp. 120-132Ashman, A., Bruins, P., Prevention of alveolar bone loss postextraction with HTR grafting material (1985) Oral Surg Oral Med Oral Pathol, 60, pp. 146-153Hjorting-hansen, E., Adawy, A.M., Hillerup, S., The pattern of postoperative bone resorption following mandibular vestibulolingual sulcoplasty with free skin graft (1983) J Oral Maxillofac Surg, 41, pp. 358-364Piecuch, J.F., Segal, D., Grasso, J.E., Augmentation of the atrophic maxilla with interpositional autogenous bone grafts (1984) Maxillofac Surg, 12, pp. 133-138Misch, C.M., Comparison of intraoral donor sites for onlay grafting prior to implant placement (1997) Int J Oral Maxillofac Implants, 12, pp. 767-776Garg, A.K., Morales, M.J., Navarro, I., Duarte, F., Autogenous mandibular bone grafts in the treatment of the resorbed maxillary anterior alveolar ridge: rationale and approach (1998) Implant Dent, 7, pp. 169-176Lekholm, U., Gunne, J., Henry, P., Higuchi, K., Linden, U., Bergstrom, C., Steenberghe, D.V., Survival the Branemark Implant in partially. edentolous jaws: a 10-year prospective multicenter study (1999) Int J Oral and Maxillofac Implants, 14, pp. 639-645Keller, E.E., Eckert, S.E., Tolman, D.E., Maxillary antral and nasal one-stage inlay composite bone graft: preliminary report on 30 recipient sites (1994) J Oral Maxillofac Surg, 52, pp. 438-447Branemark, P.I., Lindstrom, J., Hallén, O., Breine, U., Jeppson, P.-h., Ohman, A., Reconstruction of the defective mandible (1975) Scand J Plast Reconstr Surg, 9, pp. 116-128Triplett, R.G., Schow, S.R., Autologous bone grafts and endosseous implants: complementary techniques (1996) J Oral Maxillofac Surg, 54, pp. 486-494Montazem, A., Valauri, D.V., St-Hilaire, H., Buchbinder, D., The mandibular symphysis as a donor site in maxillofacial bone grafting: a quantitative anatomic study (2000) J Oral Maxillofac Surg, 58, pp. 1368-1371Raghoebar, G.M., Batenburg, R.H.K., Vissink, A., Reintsema, H., Augmentation of localized defects of the anterior maxillary ridge with autogenous bone before insertion of implants (1996) J Oral Maxillofac Surg, 54, pp. 1180-1185Raghoebar, G.M., Meijndert, L., Kalk, W.W.I., Vissink, A., Morbidity of mandibular bone harvesting: a comparative study (2007) Int J Oral Maxillofac Implants, 22, pp. 359-365Serra E Silva, F.M., Cortez, A.L.V., Moreira, R.W.F., Mazzonetto, R., Complications of intraoral donor site for bone grafting prior to implant placement (2006) Implant Dent, 15, pp. 420-426Misch, C.E., (2000) Implantes dentários contemporãneos, , 2 ed. São Paulo: SantosBezerra, F.J.B., Lenharo, A., (2000) Técnica clínica avançada em implantodontia, , São Paulo: Artes MédicasClavero, J., Lundgren, S., Ramus or chin grafts for maxillary sinus inlay and local onlay augmentation: comparison of donor site morbidity and complications (2003) Clin Implant Dent Relat Res, 5, pp. 154-160Joshi, A., An investigation of post-operative morbidity following chin graft surgery (2004) Br Dent J, 196, pp. 215-218Block, M.S., Kent, J.N., Kallukaran, F.U., Thunthy, K., Weinberg, R., Bone maintenance 5 to 10 years after sinus grafting (1998) J Oral Maxillofac Surg, 56, pp. 706-71

Expression Of A Thioredoxin Peroxidase In Insulin-producing Cells

The presence of thioredoxin peroxidase (TPx), also known as thiol specific antioxidant (TSA), was investigated in neonatal and adult rat islets, and in the β-cell line HIT-T15. Western blotting of extracts from neonatal and adult pancreatic islets and from the tumoral cell line HIT-T15 revealed the presence of a 25 kDa protein that comigrated with purified yeast TPx. Endocrine pancreatic TPx accounted for approximately 0.01% of the total protein content. Treatment with H 2O 2 for 3 h increased the expression of TPx in HIT-T15 cells. The distribution of TPx throughout the islet cells was confirmed by immunocytochemistry. Since pancreatic β-cells possess a weak antioxidant enzyme defense system, especially with regard to hydrogen peroxidase-decomposing enzymes, the presence of a TPx analog in islets suggests that this enzyme may play a role in protecting pancreatic cells against reactive oxygen species.286 II3S253S28Malaisse, W.J., Malaisse-Lagae, F., Sener, A., Determinants of the selective toxicity of alloxan to the pancreatic β-cell (1982) Proc Natl Acad Sci USA, 79, pp. 927-930Tiedge, M., Lortz, S., Drinkgem, J., Relation between antioxidant enzyme gene expression and antioxidative defense status of insulin producing cells (1997) Diabetes, 46, pp. 1733-1742Eizirik, D.L., Flodstrom, M., Karlsen, A.E., The harmony of the spheres: Inducible nitric oxide synthase and related genes in pancreatic beta cells (1996) Diabetologia, 39, pp. 875-890Kim, K., Kim, I.H., Lee, K.Y., The isolation and purification of a specific "protector" protein which inhibits enzyme inactivation by a thiol/Fe(III)/O 2 mixed function oxidation system (1988) J Biol Chem, 263, pp. 4704-4710Kim, M., Kim, K., Rhee, S.G., Induction of an antioxidant protein of Saccharomyces cerevisiae by O 2, Fe 3-, or 2-mecaptoethanol (1989) Proc Natl Acad Sci USA, 86, pp. 6018-6022Netto, L.E.S., Chae, H.Z., Kang, S.W., Removal of hydrogen peroxide by thiol-specific antioxidant enzyme (TSA) is involved with its antioxidant properties (1996) J Biol Chem, 271, pp. 15315-15321Zhang, P., Liu, B., Kang, S.W., Thioredoxin peroxidase is a novel inhibitor of apoptosis with a mechanism distinct from that of Bcl-2 (1997) J Biol Chem, 272, pp. 30615-30618Hotta, M., Tashiro, F., Ikegami, H., Pancreatic beta cell-specific expression of thioredoxin, an antioxidative and antiapoptotic protein, prevents autoimmune and streptozotocin-induced diabetes (1998) J Expl Med, 188, pp. 1445-1451Chae, H.Z., Uhm, T.B., Rhee, S.G., Dimerization of thiol-specific antioxidant and the essential role of cysteine 47 (1994) Proc Natl Acad Sci USA, 91, pp. 7022-7026Chen, H., Carlson, E.C., Pellet, L., Overexpression of metallothionein in pancreatic beta-cells reduces streptozotocin-induced DNA damage and diabetes (2001) Diabetes, 50, pp. 2040-204