Nonylphenol-degrading Microbe

東京水産大学生物資源化学講座東京水産大学生物資源化学講座東京水産大学生物資源化学講

温泉排水由来の酵母による廃糖蜜からエタノール生産ならびに,55℃でエタノール発酵を認めた新規酵母株の分子系統解析

東京水産大学食品生産学科東京水産大学食品生産学科東京水産大学食品生産学

水圏環境における耐塩性・発酵性酵母の分布

東京水産大学生物資源化学講座東京水産大学生物資源化学講座東京水産大学生物資源化学講

Application of Photon Correlation Spectroscope Technique to Rapid Detection of Nitrifying Bacteria

Rapid detection system for nitrifying bacteria was developed using photon correlation spectroscope（PCS）technique．The method is based on that nitrifダing bacterial cells reacted with anti－nitrifying bacteria antibody were able to analyze by PCS technique．When nitrifying bacterial cells were reacted with the antibody beads，the size distribution was shifted to larger rangeIn contrast，in the reference bacterial cells，the size distribution shows little change.The phenomenon was assumed that since the antibody reacted with nitrifンing bacteria selectively，they were aggregated with bacterial cells，and the distribution size increased.Thus，it is possible to discriminαte between aimed nitrifヅing bacteria and other species of bacterial cells using PCS technique.One assay could be completed in IO min，and the total PCS assay time includlng sample preparation waswithin 60min

Enzyme sensor system for determination of total cholesterol in fish plasma

An enzyme biosensor system was developed for rapid and continuous determination of total cholesterol in fish plasma. The system was constructed with an immobilized enzyme membrane, Clark-type oxygen electrode, flow system and personal computer. The enzyme membrane was prepared from cholesterol esterase, cholesterol oxidase, photosensitive polymer and an ultra-thin dialysis membrane.The optimum assay conditions of the sensor system were established as follows: pH 6.8, temperature 25°C and flow rate 0.2 mL/min. The calibration curves were linear in the range of 15-80 mg/dL for yellowtail, 17-42 mg/dL for amberjack and 20-70 mg/dL for rainbow trout. One assay was completed within 5 min and it was possible to determine the sample continuously. The sensor system was applied to the determination of total cholesterol in yellowtail plasma. A good correlation was observed between the values determined by the sensor method and the conventional method (correlation coefficient was 0.90)