Demonstration of dnp groups on the draining lymph node cells of guinea pigs following skin painting with DNCB by peroxidase labelled antibody method.

The distribution of 2,4-dinitrophenyl (DNP) groups in the draining lymph nodes of guinea pigs 12 h after painting the skin with 2,4-dinitrochlorobenzene (DNCB) was examined by a peroxidase labelled antibody method using antibody against DNP groups. DNP groups were detected on cells that were found mainly in the subcapsular sinus of the lymph nodes. Electron microscopic examination showed DNP groups distributed on the surface of lymphocytes. The significance of these findings is discussed.</p

An Attempt to Suppress the Induction of Contact Sensitivity to 2,4-Dinitrochlorobenzene by Tape Stripping Treatment of Guinea Pig Skin

The effect of stripping treatment with cellophane tape on induction of contact sensitivity (CS) to 2,4-dinitrochlorobenzene (DNCB) was studied in inbred JY1 strain guinea pigs in order to determine whether Langerhans cells (LC) are relevant to production of CS. Tape stripping of ear skin achieved, to a considerable degree but not absolutely, depletion of epidermal LC, as measured by cell surface of LC dedetcted by ATPase staining. However, pretreatment of tape stripping on the induction site of contact sensitization with DNCB did not diminish the rate and intensity of challenge reactions to DNCB

The Effect of Tape Stripping Treatment of Guinea Pig Skin on the Induction of Delayed Hypersensitivity

The induction of CS to DNCB was impaired if DNP-EC was injected subcutaneously through the skin that was stripped with cellophane tape immediately before the antigen application. The suppressive effect of tape stripping on DNP-EC induced CS was abrogated when normal PM was mixed and injected subcutaneously with DNP-EC into stripped skin. By contrast, tape stripping treatment before BCG or OVA in FIA administration had not influence on development of these delayed hypersensitivity. Significance of these findings is discussed