Adaptation à la sécheresse et création variétale : le cas de l’arachide en zone sahélienne

Drought is a multiform constraint whose impact on the vegetal metabolism is very variable according to its duration, intensity and phenological stage of the vegetal development where it occurs. Thus, the plant resistance is expressed at different plant organisation levels. The present study was aimed at integrating knowledge generated by experiments carried out in Senegal on groundnut within the framework of a breeding programme geared towards improving groundnut yield under drought conditions. Three studies involved in the breeding work, are presented. The first chapter analyses an incomplete half-diallel cross performed on an original population under recurrent selection for drought adaptation. The study confirmed the weak heritability of yields but concludes that the best predictor of pod-yield was the pod-yield itself. By contrast, the study of the genetic correlations showed that a selection for high haulm-yield could lead to poor pod-maturity under drought constraint. The selection indices were performed and used to estimate genetic gains relative to the main agronomic characters according to selection pressure. The second chapter covers the genetic variability of phenological, agronomic and physiological characters studied in two series of quasi-isogenic early lines. It has indicated that genetic variability was expressed in these lines despite its closeness. Some correlations between yield and physiological parameters, i.e. mainly fluorescence parameters, were significant but not stable across lines and environments showing that groundnut have different drought adaptation strategies according to genetic background and drought pattern. This work was pursued at the molecular level with three reference cvs involving the both recurrent parents of the precedent study. The gene transcript kinetics under drought, obtained using RT-PCR, showed that Phospolipase D and Cysteine protease gene expressions were stimulated by stress in the most susceptible cultivars, whereas their was higher LEA gene expression in the resistant one. These interconnected experiments conducted at different plant organisation levels led to the development of a general methodological model and of new improved genotypes to meet the social demand

Risk Factors for Thrombosis in an African Population

Little is known about the biological, epidemiological, and clinical risk factors for thrombosis and venous thromboembolism (VTE) among Black Africans. We undertook a study of the prevalence of VTE risk factors for thrombosis in a Senegalese population. A three-year cross-sectional and case-control study involving 105 cases and 200 controls was conducted in various hospitals in Dakar (Senegal). Our results demonstrate that oral contraception, immobilization by casts, surgery, and blood group were significantly associated with VTE occurrence. Additionally, 16 cases and 2 controls had protein S (PS) values of less than 48.4% (M-2SD), exhibiting a highly significant difference ( P < 1 x 10 −4 ). The number of cases with a low protein C (PC) level was significantly higher than the respective number of controls. Using logistic regression methods, we established a correlation between significantly associated variables and deep venous thrombosis (DVT) occurrence. Age, obesity, sickle cell disease, and PC deficiency were not significantly associated with thrombosis. In contrast, gender, PS deficiency, varicose veins, surgery, non-O blood type, and the presence of anti-phospholipid antibodies were significantly and independently associated with DVT. These findings are extremely useful for clinical management of patients suffering from DVT and can help to reduce the high recurrence rate observed in our study