Opinions of Future Prospective Teachers on Coding Technique

© 2020. iJET. All Rights Reserved. The use of technology in education has increased rapidly in recent years, and one of these is coding training. Rapid changes have been observed in coding education in recent years. Many countries have added coding-related lessons to their curricula. There are studies about coding education that realise the learning of individuals at a very young age. This rapid change raises many questions for researchers. In this rapidly developing age, the views of prospective teacher candidates on coding are very important. With this study, it is aimed to discover the thoughts of prospective teachers of the Computer and Instructional Technologies Education department on coding education. For this purpose, qualitative data were obtained from 25 pre-service Computer and Instructional Technologies Education teacher candidates with case study method in Northern Cypriot, Russian and Kazakhstani universities. Selections were made based on volunteering among the pre-service teachers who took coding lessons. In this study, the data were collected with the semi-structured interview technique, which was developed considering the purpose of the research. Qualitative content analysis was used in the analysis of the data obtained. The results obtained in the research process revealed that the needs of pre-service teachers regarding the coding education were lacking and the number of coding courses should be increased

Identification of Antimicrobial Peptides from Novel Lactobacillus fermentum Strain

© 2020, Springer Science+Business Media, LLC, part of Springer Nature. Antimicrobial peptides (AMPs) are natural antagonistic tools of many bacteria and are considered as attractive antimicrobial agents for the treatment of bacteria with multidrug resistance. Lactic acid bacteria from the gastrointestinal tract of animals and human produce various AMPs inhibiting the growth of pathogens. Here we report the isolation and identification of novel Lactobacillus fermentum strain HF-D1 from the human gut producing AMPs which prevents the growth of P. aeruginosa and S. marcescens. The active fraction of peptides was obtained from the culture liquid by precipitation at 80% saturation of ammonium sulphate. For peptides identification, the precipitate was treated with guanidine hydrochloride to desorb from proteins, separated with ultrafiltration on spin columns with 10,000 MWCO, desalted with a reversed-phase chromatography and subjected to LC–MS/MS analysis. The in silico analysis of the identified 1111 peptides by using ADAM, CAMPR3 and AMPA prediction servers led to identification of the linear peptide with highly probable antimicrobial activity and further investigation of its antibacterial activity mechanism is promising. By using the dereplication algorithm, the peptide highly similar to non-ribosomal cyclic AMPs originally isolated from Staphylococcus epidermidis has been identified. This indicates that L. fermentum HF-D1 represents a novel strain producing antimicrobial peptides targeting P. aeruginosa and S. marcescens