ABROGATION OF CARBON TETRACHLORIDE (CCL4) INDUCED HEPATOTOXICTY BY AROGYAVARDHINI IN WISTAR RATS.

From long back onwards Arogyavardhini, a herbo-mineral preparation  is used in the affections of liver & spleen disorders as a ayurvedic preparation.The present study was aimed to evaulate the  hepatoprotective effect of arogyavardhini in carbon tetrachloride (CCl4) induced liver damage in wistar  rats. In the present study Arogyavardhini A (65 mg/kg, p.o) and Arogyavardhini B (65 mg/kg, p.o) were used to screen the hepatoprotective activity. Hepatotoxicity was induced by the CCl4 ( 3 ml/kg, p.o), and silymarin (50 mg/kg, p.o) was taken as a standard. Biochemical parameters like serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate trasaminase (SGPT), alkaline phosphatase (ALP), total bilirubin and direct bilirubin levels were estimated. Histapathological examination of liver samples were also done. CCl4 treated groups showed the elevated levels of biochemical parameters like SGOT, SGPT, ALP, total bilirubin, and direct bilirubin levels. In-case of Arogyavardhini treated groups significantly (p<0.01) prevented this hepatotoxicity. Histopathological examinations revealed the post-treatment of Arogyavardhini exhibited the protection of liver tissue from CCl4 induced hepatotoxicity. The observed results strongly supports the hepatoprotective activity of arogyavardhini against CCl4 induced hepatotoxicity.   Keywords: Arogyavardhini, CCl4, Hepatotoxicity, SGOT, SGPT

Evaluation of safety and efficacy of Maa-Lact in lactating Holtzman rats

Objective: To evaluate the safety & efficacy of Maa-Lact granules for its galactogougue activity in Holtzman rats and its effect on suckling pups. Methods: Group I rats were treated as control, group II and III rats were treated with 500 mg/kg, 1 000 mg/kg of Maa-Lact granules for 21 days. Weekly body weights of dams and pups were collected, litter survivability for 22 days and ocular blood samples were collected on 1st day of parturition and 21st day of post parturition for the estimation of prolactin levels. On 21st day blood samples were collected from retro-orbital sinus for haemotological and biochemical estimations. On the same day of weaning rats were sacrificed and subjected to necropsy and individual organ weights were recorded. Results: No significant difference in weekly food weight consumption, body weights between control & treated groups with normal clinical signs. There is no mortaly in dams throught the study period with no significant difference in pups weights. The percentage mortality in pups was 14.43 %, 14.07 %, and 13.42% in group I, group II and group III, respectively. The histopathological finding has shown that treated groups have less convulution and adipose tissue deposition along with increase in length and branching of lactiferous duct and alveolar size. Conclusion: Based on above results, it can be concluded that Maa-Lact posseses significant galctogogue activity