Internal organs morphology and tumors in laboratory rats with transplanted liver cancer PC-1 by oral intoduction containing extract of Gratiola (Gratiola Officinalis L.) and Anthocyan Maize (Zea Mays L.)

The purpose of the work is to study antitumor activity of flavonoid-containing extracts of anthocyan maize and gratiola in experiments in vivo and to estimate their effects on the internal organs, tumors and blood of rats with transplanted liver cancer PC-1. Materials and methods: Effect of extracts of anthocyan maize and gratiola has been studied in experiments in vivo in 30 laboratory rats with transplanted liver cancer PC-1 using morphological and biochemical methods. Results: It has been found that extract of gratiola received by a certain way has selective cytotoxic effect on tumor cells. Anthocyan maize extract reduces endogenous intoxication. Conclusion: These results open the prospect of further study of the extract of gratiola in various experimental models of cancer and anthocyan maize extract use may be developed as a means of endogenous intoxication reducing for application in the treatment of cancer. Investigation of anthocyan maize extract may assist in the development of drugs that reduce the endogenous intoxication at the treatment of cancer

Evaluation of the wound healing capacity of the gel form of the preparation "Mellisol" on the model of experimental planar wounds

Objective: to study the wound healing activity of the gel form of the "Mellisol" preparation containing nanoaggregates of flavonoids stabilized with polyazolidine ammonium modified with iodine hydrate ions on a model of experimental planar wounds of rats. Material and Methods. An investigation of the wound healing activity of the gel form of the "Mellisol" preparation was carried out in vivo in 18 white laboratory rats of the Wistar line. Simulation of experimental planar wounds was performed according to the standard procedure described in the preclinical testing manual. Results. In the course of the studies, it was established that a statistically significant (p=0.045) acceleration of healing on the 12th day of the experiment occurred in animals of the experimental group on whose wounds the gel form of the cosmetic means "Mellisol" was applied, and the complete healing of the wound defect occurred on the 21st day after operation. Conclusion. The gel form of the preparation "Mellisol" is characterized by a high level of antimicrobial and regenerative activity reliably reducing the healing time for planar wounds without the development of microbial complications.</p

Morphological changes in the organs of laboratory animals during the prolonged oral administration of gold nanoparticles

The purpose of the article is to assess the severity of the morphological changes in the internal organs of laboratory animals with prolonged oral administration of gold nanoparticles of different sizes. Material and methods: The experiment was carried out on 40 albino rats which were divided into 4 groups. Animals of the experimental group received oral gold nanoparticles by the appropriate scheme. Results: At comparing the morphological changes in the internal organs of laboratory animals it was established that the introduction of gold nanoparticles of different sizes leads to the same type of disturbances of morphological structure, the gold nanoparticles of 1-3 and 50 nm cause the most expressive changes. Conclusion: The most significant morphological modifications in the internal organs were caused by gold nanoparticles of 1-3 and 50 nm. It points out to the size-dependence of the effect of gold nanoparticles

Effect of Systemic Polyelectrolyte Microcapsule Administration on the Blood Flow Dynamics of Vital Organs

Polyelectrolyte microcapsules and other targeted drug delivery systems could substantially reduce the side effects of drug and overall toxicity. At the same time, the cardiovascular system is a unique transport avenue that can deliver drug carriers to any tissue and organ. However, one of the most important potential problems of drug carrier systemic administration in clinical practice is that the carriers might cause circulatory disorders, the development of pulmonary embolism, ischemia, and tissue necrosis due to the blockage of small capillaries. Thus, the presented work aims to find out the processes occurring in the bloodstream after the systemic injection of polyelectrolyte capsules that are 5 μm in size. It was shown that 1 min after injection, the number of circulating capsules decreases several times, and after 15 min less than 1% of the injected dose is registered in the blood. By this time, most capsules accumulate in the lungs, liver, and kidneys. However, magnetic field action could slightly increase the accumulation of capsules in the region-of-interest. For the first time, we have investigated the real-time blood flow changes in vital organs in vivo after intravenous injection of microcapsules using a laser speckle contrast imaging system. We have demonstrated that the organism can adapt to the emergence of drug carriers in the blood and their accumulation in the vessels of vital organs. Additionally, we have evaluated the safety of the intravenous administration of various doses of microcapsules. © 2019 American Chemical Society