Редкое наблюдение разрыва аневризмы брюшной аорты с формированием тромбированной аортокавальной фистулы, тромбоза нижней полой и подвздошных вен

This article considers a rare case of rupture of abdominal aortic aneurysm with thrombosed aorto-caval fistula formation, with thrombosis of the inferior vena cava and iliac veins. During the multiphase computed tomography, early enhancement of inferior vena cava was not obtained, but non-contrasting aorto-caval fistula was revealed in the venous contrast phase, as well as the absence of the contrast in the adjacent parts of the inferior vena cava and iliac veins. The patient was successfully operated and the diagnosis was confirmed intraoperatively.Представлено редкое клиническое наблюдение диагностики разрыва аневризмы брюшной аорты с образованием тромбированной аортокавальной фистулы в сочетании с тромбозом нижней полой вены (НПВ) и подвздошных вен. В ходе выполнения мультифазной компьютерной томо­графии раннего сброса контрастного препарата из аорты в НПВ получено не было, но в венозную фазу контрастирования выявлено неконтрастирующее аортокавальное соустье, а также отсут­ствие контрастирования прилежащих отделов НПВ и подвздошных вен. Пациент был успешно прооперирован, диагноз был подтвержден интраоперационно

Concerning the issue of early diagnostics of low tannin content in faba bean seeds (<i>Vicia faba</i> L.)

Background. The use of faba bean (Vicia faba L.) as a feed and food plant is hindered by the presence of antinutritional substances and in particular phenolic compounds – tannins – in the seed coat. The existence of low-tannin or zero-tannin forms in the faba bean gene pool allows breeders to promote this trait. The aim of this work was to carry out biochemical validation of a known morphological marker of low tannin content and identify relationships of some phenotypic traits characterized by accumulation of phenolic compounds (tannins and anthocyanins) with their concentration for use as additional morphological markers of the low content of these antinutrients in bean seeds.Materials and methods. Associations of the presence/absence of anthocyanin in seedlings, pigmentation on petals, and dark extrafloral nectaries on stipules with the content of tannins and anthocyanins were analyzed in 10 faba bean accessions with different seed coat colors from the VIR collection. The plants were grown and evaluated in Leningrad Province in 2020–2021. Tannins in seeds were assessed using Leventhal’s method. Anthocyanins were analyzed in the green biomass of plants using a spectrophotometric method.Results and conclusion. Different combinations of the studied morphological features associated with the coloration of the studied organs were found. A high positive correlation between the levels of anthocyanins and tannins (r = 0.79) was identified as well as a direct connection of the high value of these traits with the presence of a dark extrafloral nectary on stipules and anthocyanin in seedlings. The absence of anthocyanin staining on seedlings and the absence of dark-colored extrafloral nectaries on stipules can serve as markers of low-tannin genotypes in the early stages of plant development. The light seed coat color that does not darken for a long time may also be the evidence of low tannin content

Rapid assessment of the main economic value indicators in lupine flour samples using infrared spectroscopy

Background. A calibration model has been developed for rapid assessment of economic value indicators (protein, oil, and quinolizidine alkaloid contents) in the seeds of narrowleaf lupine accessions from VIR using near-infrared spectroscopy, with the help of which it is possible to decide on the further use of the accessions.Materials and methods. Biochemical quality indicators (protein, oil, and quinolizidine alkaloid content) were studied in the seeds of narrowleaf lupine (Lupinus angustifolius L.) grown in 2019 in the northwest of Russia. Calibration models for measuring protein, oil and alkaloids in lupine seeds (62 accessions) were developed using a MATRIX-I IR analyzer (Bruker Optics, Germany). To construct calibration models, we used the values obtained by chemical analysis methods accepted at VIR. The oil content in lupine seeds was assessed by the defatted dry residue technique in Soxhlet extractors, protein by the Kjeldahl method, and quinolizidine alkaloids by gas chromatography coupled with mass spectrometry. All indicators were recalculated on the dry-weight basis.Results and conclusion. Statistical significance of the developed models was verified according to the results of measuring the content of protein, oil and alkaloids in the seeds of the test batch. The protein and oil content data obtained using a calibration curve did not differ significantly from the results of chemical studies, in contrast to alkaloid indicators. Consequently, the developed calibration model for the MATRIX-I IR analyzer can be used for rapid assessment of protein and oil content in narrowleaf lupine flour samples, thus accelerating the process of obtaining data on the main economic value indicators. The analysis does not require reagents and is safe

Impact of weather and climate on seed protein and oil content of soybean in the North Сaucasus

For a targeted search of initial breeding material for the quality of soybean seeds, it is necessary to know the patterns of the dependence of the corresponding seed characters on the weather and climatic conditions in a particular region. Global climatic change, the concretization of which is relevant, has a share in this dependence. Thus, the aim of this work was to identify the relationship between the variability of protein and oil content in soybean seeds with climatic parameters in the North Caucasus as well as trends in this variability over a long time period. The study of 1 442 soybean accessions from VIR collection in the Krasnodar region during 1987–2015 had been carried out and the tendencies of the variability of protein and oil content in seeds in this environment were estimated. The regression analysis in differences with forward stepwise selection of variables has been used to construct models for the dependence of the protein and oil content on generalized agrometeorological indices. During 1987–2015, for the period with temperatures above 10 °C, the sums of active temperatures increased by 218 °C/10 years and precipitation decreased by 20.9 mm/10 years. In the dynamics of protein content, a trend has been revealed as an increase by 2.5 % over 10 years, while there is no reliable trend in oil content. The maximum average mean of oil content and the smallest protein were in the middle-maturing accessions (22.2 and 38.8 %), and a relatively high protein content was detected, on average, in the early- (21.6 and 40.0 %) and late-maturing (20.2 and 39.9 %) varieties. The protein content had been increasing with a growth of the duration of the period with temperatures above 22 °C and decreasing with a raise in precipitation over a period of temperatures above 18 °C. The accumulation of oil in seeds was promoted by an increase of the hydrothermal coefficient over the period with temperatures above 19 °C, and, in late-maturating varieties, prevented by a prolonged autumn period with temperatures below 15 °C. Long-term growth in protein content is due to both climatic change and genetic improvement of varieties

SPECIES DIVERSITY OF THE VIR COLLECTION OF GRAIN LEGUME GENETIC RESOURCES AND ITS USE IN DOMESTIC BREEDING

The world’s genebanks hold 7.5 million germplasm accessions of plant genetic resources (PGR). One of the qualitative characteristics of the PGR collections is the species diversity, in particular, the presence of crop wild relatives (CWR), which makes it possible to widen the use of gene pools in the breeding process. The collection of the Vavilov Institute (VIR) is one of the most diverse holdings in the number of plant species. A survey is provided here of the species diversity in VIR’s grain legume collection, and its use in domestic breeding practice is analyzed. Comparison of this diversity with the state of PGR exploitation in the world makes it possible to assess the prospects of more efficient utilization of gene pool potential, especially for species that are unjustifiably cultivated on a too small scale or even neglected as crops in this country. The VIR collection of grain legumes incorporates 196 species from 9 genera of the family Fabaceae. This number includes cultigens and CWR. The cultivars of 21 species of grain legumes listed in the State Register of Breeding Achievements (2018) are adapted to the soil and climate conditions of this country. However, the species diversity of the collection could be used more efficiently in domestic plant breeding and crop production. This concerns both underutilized crops in Russia (broad beans, lima beans and grass pea) and those whose adaptive potential is adjusted only to certain and limited areas of the Russian Federation (Tepary beans and Vigna spp.). It is also necessary to exploit more efficiently species of the wild flora, both for direct utilization as pastures, green manure or phytoremediation crops and for introgressive breeding and domestication (Vicia benghalensis L., V. narbonensis L., Lathyrus sylvestris L., Lupinus hartwegii Lindl., etc.). Incorporation of crop wild relatives into the breeding process is promising for crop improvement in a number of aspects: for example, to increase resistance to diseases, pests, abiotic stressors, etc

Selection of an optimal method for screening the collection of narrow-leaved lupine held by the Vavilov Institute for the qualitative and quantitative composition of seed alkaloids

Narrow-leaved lupine (Lupinus аngustifolius L.) is a widely cultivated leguminous forage and green manure crop with a potential for human nutrition. However, the presence of secondary metabolites – alkaloids – in lupine seeds considerably affects the quality of raw produce, reducing its nutritive value; in addition, high concentrations of alkaloids are toxic to humans and animals. Therefore, plant breeders working with lupine need to gain knowledge about the variability of alkaloid content in seeds of different genotypes and search for the sources of their low concentrations in the crop’s gene pool. The collection of narrow-leaved lupine genetic resources held by the N.I. Vavilov Institute of Plant Genetic Resources (VIR) offers wide opportunities for such search by means of mass screening. For its part, largescale gene pool screening requires the selection of an optimal technique to measure alkaloid content in seeds, so that it would be easily reproducible and as little labor-, time- and fund-consuming as possible. The results of the search for such method are presented. Qualitative and quantitative indices were compared when target compounds had been extracted with multicomponent mixtures and individual reagents (chloroform, methanol, etc.) and the extracts analyzed using gas chromatography-mass spectrometry. High-performance liquid chromatography combined with mass spectrometry was also employed. Five major alkaloids were found to be present in all types of extracts: lupanine, 13-hydroxylupanine (dominant ones), angustifoline, sparteine, and isolupanine. The fullest extraction of alkaloids was observed when the extractant with an added alkaline agent was used (425 mg/100 g). The lowest level of extraction was registered with chloroform (216 mg/100 g). The significance of the differences was confirmed statistically

ИССЛЕДОВАНИЕ РАДИОАКТИВНОГО ЗАГРЯЗНЕНИЯ МОРСКОЙ БИОТЫ В СВЯЗИ С АВАРИЕЙ НА АЭС «ФУКУСИМА-1»

134Cs and 137Cs contents have been studied in 44 samples of the marine biota including four species of brown and red algae (11 samples), four species of invertebrates (8 samples) and ten species of fish (25 samples). The samples have been collected in the Northwest Pacific Ocean and in the Seas of Okhotsk and Japan within the framework of the Russian monitoring program that started in 2011 to study environmental consequences of the accident at “Fukushima-1” NPP. In 2011–2012, total activity of both cesium radioisotopes for all the samples analyzed did not exceed 1 Bq kg–1 (wet weight). This value is negligible compared to the safe level of 130 Bq kg–1 (for 137Cs) for the fish consumption in Russia. 134Cs, a marker of the Fukushima-derived contamination, has been determined at a level of 0.2–0.4 Bq kg–1 (wet weight) for three samples of pacific saury (Cololabis saira) collected from areas near Shikotan Island in Sempember 2011 and 2012. The study shows that the Fukushima accident has no considerable impact on radiation conditions in the Kuril-Kamchatka region of the Northwest Pacific Ocean and in the Russian waters of the Sea of Japan.Изучено содержание радионуклидов 134Cs и 137Cs в 44 пробах морских организмов, представленных четырьмя видами водорослей (11 проб), четырьмя видами беспозвоночных животных (8 проб) и десятью видами рыб (25 проб). Образцы морских организмов были отобраны в водах Японского и Охотского морей и северо-западной части Тихого океана в 2011–2012 гг. в рамках программы комплексных исследований по изучению последствий аварии на АЭС «Фукусима-1» для дальневосточных регионов Российской Федерации. Во всех проанализированных пробах удельная активность радионуклидов цезия (по сумме двух изотопов) не превышала 1 Бк/кг (на сырой вес). Присутствие 134Cs – маркера «фукусимских» выбросов и сбросов – было достоверно обнаружено в трех пробах тихоокеанской сайры (Cololabis saira), выловленной у Южных Курил в 2011 и 2012 гг. Проведенное исследование показывает, что авария на АЭС «Фукусима-1» не оказывает заметного воздействия на радиационную обстановку в Японском море и Курило-Камчатском районе Тихого океана

Анализ влияния особенностей радиационных аварий на кризисную риск-коммуникацию

In the event of radiation accidents and incidents related to a possible threat to public health, one of the tools for forming health-saving behavior of the population, ensuring the social acceptability of protective measures and reducing social tension is crisis communication. Nuclear and radiation emergencies associated with the potential for radioactive contamination of large areas or the impact on the population can become the basis for the emergence of resonant information events. Properly organized crisis communication with the population in such situations is one of the necessary conditions for the effectiveness of emergency response. The article analyzes the impact on crisis risk communication of some features of nuclear and radiation events that have occurred in recent years. These events have had a significant impact on the possibility or impossibility of following the general principles of crisis communication, and provides examples of recommendations for adjusting information work if it is impossible to strictly follow the general principles of crisis communication in real circumstances. The study made it possible to identify the following features of the development of specific crisis situations that influenced the course of crisis communication and created a number of difficulties: 1) the latent nature of the early phase of the accident; 2) absence or deliberate concealment of information about a radiation accident; 3) the transboundary nature of the accident; 4) hidden (secret) nature of information about the accident; 5) an increase in the level of radiation anxiety among the population of the territories not affected by the accident; 6) the rapid formation and spread of myths; 7) projecting artistic images on a real accident. The analysis of the influence of the features of radiation accidents and incidents on the crisis communication allowed us to come to the following conclusions: 1) crisis communication is an important component of emergency response, affecting both the psychoemotional state of the population and the effectiveness of emergency measures related to the actions or inaction of the population; 2) the development of means of information interaction and methods of dissemination and consumption of information requires a revision of traditional methods of information work with the population; 3) the information should be timely, objective, consistent and understandable both for the public and for non-specialists in the field of radiation hygiene responsible for making management decisions; 4) information should not be presented in the form of short messages without explanations; 5) contradictory aggressive information received by the population from different sources may cause distrust of official sources of information; 6) if you do not provide up-to-date and objective information about the accident, the public’s trust is very easy to lose and very difficult to restore.При возникновении радиационных аварий и инцидентов, связанных с возможной угрозой для здоровья населения, одним из инструментов формирования здоровьесберегающего поведения населения, обеспечения социальной приемлемости защитных мероприятий и снижения социальной напряжённости является кризисная риск-коммуникация. Радиационные аварии и инциденты, связанные с потенциальной возможностью радиационного загрязнения значительных по площади территорий или воздействия на население, могут стать основой для возникновения резонансных информационных поводов. Должным образом организованная информационная работа с населением в подобных ситуациях является одним из необходимых условий эффективности противоаварийных мероприятий в целом. В статье проанализировано влияние на кризисную риск-коммуникацию некоторых особенностей радиационных аварий и инцидентов, произошедших в последние годы, оказавших существенное влияние на возможность или невозможность следования общим принципам кризисной коммуникации; приводятся примеры рекомендаций по корректировке информационной работы при невозможности строгого следования общим принципам кризисной коммуникации в реально сложившихся обстоятельствах. В ходе исследования были выявлены следующие особенности развития конкретных радиационных аварий и инцидентов, оказавшие влияние на ход кризисной коммуникации и создавшие ряд сложностей для специалистов: 1) скрытый характер ранней фазы аварии; 2) отсутствие или умышленное сокрытие сведений о радиационной аварии; 3) трансграничный характер аварии; 4) закрытый (секретный) характер сведений об аварии; 5) повышение уровня радиотревожности у населения территорий, не затронутых аварией; 6) быстрое формирование и распространение мифов; 7) проецирование художественных образов на реальную аварию. Анализ влияния особенностей радиационных аварий и инцидентов на кризисную риск-коммуникацию позволил прийти к следующим выводам: 1) кризисная риск-коммуникация является важной составляющей аварийного реагирования, влияющей как на психоэмоциональное состояние населения, так и на эффективность противоаварийных мероприятий, связанных с действиями или бездействием населения; 2) развитие средств информационного взаимодействия и способов распространения и потребления информации требует пересмотра традиционных методов информационной работы с населением; 3) информация должна быть своевременной, объективной, непротиворечивой и понятной как для населения, так и для неспециалистов в области радиационной гигиены, ответственных за принятие управленческих решений; 4) информация не должна представляться в виде кратких сообщений без объяснений; 5) противоречивая агрессивная информация, получаемая населением из разных источников, может вызвать недоверие к официальным источникам информации; 6) при непредставлении актуальной и объективной информации о происходящей аварии доверие населения очень легко потерять и очень трудно восстановить

ПРОБЛЕМЫ РИСК – КОММУНИКАЦИИ: ОБЩЕСТВЕННЫЕ ОРГАНИЗАЦИИ И ИХ РОЛЬ В ФОРМИРОВАНИИ ОБЩЕСТВЕННОГО МНЕНИЯ ПО ВОПРОСАМ РАДИАЦИОННОЙ БЕЗОПАСНОСТИ НАСЕЛЕНИЯ

Events connected with the development and improvement of the nuclear industry are usually ambiguously perceived by the public. Mass-media and Internet play a leading role in the development of the public opinion on the most relevant public life issues, ecology and radiation safety in particular. Non-governmental organizations can impact the adequate perception of the issues of the radiation safety in the region by the public. Materials and methods: this study is focused on the primary evaluation of the data on the results of the sociological survey of three regions of the North-West of the Russian Federation: St-Petersburg, Leningrad and Murmansk regions; and the assessment of the websites of the non-governmental organizations, activities of the non-governmental organizations in the Internet and social networks. Results: the study indicated that non-governmental organizations actively use Internet and social networks in their practice. All the relevant non-governmental organizations have websites and social network accounts. Based on the number of the subscribers in social networks, it was possible to mark two non-governmental organizations: Greenpeace and World Wildlife Fund (WWF) with more than 400000 subscribers each. VKontakte social network was assessed as the most effective mean of communicating with the public; a total number of subscribers on the groups of major non-governmental organizations exceeds 500000 persons. These two organizations are the most popular and trusted by the public. Greenpeace is known to 26% of respondents; WWF – to 11%. The population of the Murmansk region is more informed about the actions of the non-governmental organizations. 48% of the respondents in the Murmansk region named at least one non-governmental organization answering the question “What non-governmental organizations active in the field of radiation protection do you know?” with the corresponding 18% and 29% in St-Petersburg and Leningrad region respectively. Nongovernmental organizations have a sufficient potential of influence on the adequate perception of the issues of the radiation protection by the public in the region; however, that potential is not fully implemented. Despite that, activities of the non-governmental organizations should be considered while developing the adequate perception of the radiation factors by the public.Мероприятия, связанные с развитием и совершенствованием атомной отрасли, как правило, воспринимаются населением неоднозначно. Ведущую роль в формировании общественного мнения по наиболее актуальным вопросам жизни общества, в частности, по вопросам экологии и радиационной безопасности играют средства массовой информации и Интернет. Общественные организации также могут оказывать влияние на адекватное восприятие населением вопросов радиационной безопасности в регионе. Материалы и методы: в ходе исследования выполнен первичный анализ данных социологического исследования в трех регионах Северо-Запада Российской Федерации: Санкт-Петербурге, Ленинградской и Мурманской областях, проведена оценка сайтов общественных организаций, активности общественных организаций в Интернете, социальных сетях. Результаты: исследование показало, что общественные организации активно используют в своей практике Интернет и социальные сети. У всех значимых общественных организаций имеются сайты и официальные аккаунты в социальных сетях. По количеству подписчиков в социальных сетях выделяются две общественные организации – «Гринпис» и Всемирный фонд дикой природы, имеющие более 400 000 подписчиков каждая. Социальная сеть «ВКонтакте» является наиболее эффективным способом коммуникации с населением, суммарное количество подписчиков на группы ключевых общественных организаций «Вконтакте» составляет более 500 тысяч человек. Эти же две организации являются как наиболее известными, так и пользующимися наибольшим доверием у населения. «Гринпис» знают 26% респондентов, Всемирный фонд дикой природы знаком 11%. На вопрос «О деятельности каких общественных организаций, занимающихся вопросами радиационной безопасности, Вы слышали?» в Мурманской области 48% респондентов назвали хотя бы одну общественную организацию, в то время как в Санкт-Петербурге и Ленинградской области 18% и 29% соответственно. Общественные организации имеют достаточный потенциал влияния на адекватное восприятие населением вопросов радиационной безопасности в регионе, однако в настоящее время данный потенциал не реализуется в полной мере. Вследствие этого активность общественных организаций должна быть объектом исследования при планировании работы по формированию адекватного восприятия радиационного фактора населением