The Flow Cytometry Study of Cellular Immunity in Rhesus Monkeys after Experimental Infection with SARS CoV 2 Virus

Cellular immunity plays an important role in the pathogenesis and formation of protective immune defense against the SARS‑CoV‑2 virus.The aim of the work was to study the cellular immunity of rhesus monkeys applying flow cytometry after experimental infection with the SARS‑CoV‑2 virus.Materials and methods. Male rhesus monkeys were intranasally inoculated with the SARS‑CoV‑2 virus, Isolate B strain and hCoV-19/Russia/SP48-1226/2020 strain (abbreviated name U-2), at a dose of 5.0 lg PFU. Using flow cytometry, the levels of 21 populations/subpopulations of mononuclear cells in the peripheral blood of animals were determined before experimental infection with the pathogen and on day 14 after infection. SARS‑CoV‑2 coronavirus RNA was assessed using real-time polymerase chain reaction. Determination of the titer of virus-neutralizing antibodies to the SARS‑CoV‑2 virus in the blood sera of animals was conducted through neutralization test evaluating the ability to suppress negative colonies.Results and discussion. Infection with Isolate B strain culture has led to an increase in the relative content of total T-lymphocytes (p˂0.2), cytotoxic T-lymphocytes (p˂0.1), as well as monocytes expressing the early activation marker CD25 (p˂0.2). The decrease in levels has been observed for total B-lymphocytes (p˂0.2) and T-helper cells (p˂0.1). Infection with the U-2 strain culture revealed an increase in the relative content of monocytes expressing the early activation marker CD25 (p˂0.2). Thus, for the first time in the Russian Federation, flow cytometry was used to study the cellular immunity of rhesus monkeys before and after experimental infection with the SARS‑CoV‑2 virus. The obtained information can be used for studying the pathogenesis of SARS‑CoV‑2 infection, course, and outcome of the disease, and developing strategies for vaccination and treatment

Features the nutritional status of children with relapsing respiratory pathology

A survey of 182 children aged 5 to 9 years in the children’s sanatorium «Duslyk» Ufa. A survey of 182 children aged 5 to 9 years in the children’s sanatorium «Duslyk» Ufa.The main group included 101 children with relapsing respiratory pathology, the control group 81 healthy children. The article presents an analysis of the actual weekly diet of children at home, modern approaches to the study of nutritional status using clinical, biochemical, anthropometric, somatometric methods, bioimpedance measurements. The actual nutrition of children with relapsing respiratory pathology was characterized by an imbalance in the content of the main nutrients. A comprehensive assessment of nutritional status revealed malnutrition in children of the main group. The aim of the study was to assess the actual nutrition, the study of nutritional status, taking into account the clinical and laboratory parameters and the component composition of the body of children in the children’s sanatorium. The inclusion of an assessment of actual nutrition and nutritional status in the survey program will make it possible to timely identify malnutrition in children with relapsing respiratory pathology, purposefully carry out preventive measures