Gel with Ectoine improves wound healing on a thermal burn model in rats

Due to the ability to create complexes of water molecules on the surface of the mucous membrane, ectoine is a promising compound for the treatment of burns. Evaluate the reparative activity of Ectoine using the thermal burn model in rats. In male rats (n=30), 2 thermal burns were counteracted under counter-anesthesia, one of which was treated by topical application of the test drugs for 7 days, and the other served as a contro

A CLINICAL CASE OF NORVEGIAN SCABIES IN A PATIENT WITH ACUTE LYMPHOBLASTIC LEUKAEMIA

A case of Norwegian scabies is described in a patient of 19 years with a lesion of the scalp, face, trunk, upper and lower extremities that occurred against the background of acute lymphoblastic leukemia. A good effect was obtained from conducting etiotropic, pathogenetic and symptomatic therapy in combination with specific treatment of the underlying disease

DYNAMIC CHANGES OF GRANULYSIN AND CATHELICIDIN IN CHILDREN AND ADOLESCENTS WITH DIFFERENT FORMS OF PULMONARY TUBERCULOSIS

Granulysin and cathelicidin, the cytolytic molecules of innate immune system are important protective factors during infection with Mycobacterium tuberculosis. We present original data concerning high levels of granulysin and cathelicidin among the group of children and adolescents with latent TB infection. Patients with tuberculosis of the respiratory system exhibit significantly lower amounts of serum cathelicidin in destructive forms, as well as granulysin levels in “minor” forms of tuberculosis before starting the specific chemotherapy. The chemotherapy performed did not influence the serum granulysin and cathelicidin contents in patients with destructive tuberculosis, whereas the patients with “minor” forms (TLN/focal tuberculosis) revealed a significant increase in granulysin content after 6 months of treatment, and same trend for cathelicidin concentrations after 3 months of chemotherapy, followed by subsequent return to baseline values

Complex estimation of mitochondrial changes of immunocompetent blood cells in pregnant women with urgent and premature birth

Inflammation is among the factors promoting development of premature rupture of the membranes (PPROM). Upon the conditions of physiological immune imbalance in pregnancy, inflammation modifies its course and can even change the immune response. Appropriate indexes may be quantitative and functional. We used a marker of mitochondrial membrane potential (MPM, Ay) as an integral index of the functional state of immunocompetent blood cells (IBC) in 159 women who were examined at 8-14 weeks of gestation; they were observed up to 34-36 weeks. Of these cohort, 121 women were referred to a comparison group. The main group (n = 46) consisted of pregnant women with PPROM at the term of 28-33 weeks. The examination was carried out according to current medical standards, with informed consent, being approved by the Ethics committee at the Khabarovsk branch of Far Eastern Scientific Centre of Physiology and Pathology of Respiration — Research Institute of Maternity and Childhood Protection. Additionally, MPM and lymphocyte populations were determined by flow cytometry. The degree of disturbed energy supply in the IBC was based on the data of simultaneous determination of lymphocyte, granulocyte and monocyte numbers with reduced MPM values (application for invention No. 2020115963), thus revealing 3 degrees of energy deficiency: 1st degree, monovariant IBC composition with reduced MPM; 2nd degree, bivariant composition, 3rd degree, total changes. A relative and absolute decrease in CD3 (72% vs 78% and 1624 vs 1980), CD8 (28% vs 33% and 651 vs 851), an increase in CD19 (14% vs 9% and 304 vs 219) were revealed in pregnant women with PPROM. When assessing MPM values in the IBC populations, a decreased proportion of women without energy deficiency from the 1st to the 2nd trimester (from 41% to 30%), due to the 3rd degree of energy deficiency (from 17% to 26%) was detected. A shift of affected pools at the 2nd degree of energy deficiency in favor of lymphocytic-granulocytic association (from 7% to 25%) from lymphocytic-monocytic compartment (from 73% to 50%) was found. From the 2nd to 3rd trimester, we have detected redistribution of granulocyte pools at the 1st degree (0 to 8%) and from the lymphocytic-granulocytic association (25% and 5%) to monocytic-granulocytic (25% and 40%). In the group with PPROM, there was a decreased proportion of pregnant women without energy deficiency (13% and 27%), as well as with the 1st and 2nd degrees (17% vs 31% and 9% vs 17%), due to the 3rd degree of energy deficiency (61% and 26 %), relative to the comparison group. The IBC pools of in the main group were redistributed at the 1st degree in favor of granulocytes (25% and 8%), at the 2nd, in favor of the lymphocytic-monocytic association (100% and 55%) from the granulocytic-monocytic (0% and 40%). Such imbalance of bioenergetic processes in the IBC can be an important factor of pathologically ongoing inflammation. These changes could be caused by both higher incidence of infections in such patients and by alloimmune interactions between mother and fetus. However, they may also determine the pathological course of inflammation. Preterm birth, which is usually caused by PPROM, is a multifactorial pathological condition. However, independent on specific triggers, the changes in energy supply of IBC, at least, may serve as a significant biomarker of probability for this disorder

Features of mitochondrial membrane potential of immunocompetent blood cells in children with chronic nonspecific lung diseases accompanied by pneumofibrosis

Pneumofibrosis is a pathological outcome of pulmonary tissue inflammation. It can complicate any lung and bronchial disorder. The mitochondrial membrane potential reflects functional state of immunocompetent blood cells that influence progression of a chronic inflammatory process. The aim of this work was to study the features of mitochondrial membrane potential (MMP) of immunocompetent blood cells in children with chronic nonspecific lung diseases (CNPD), accompanied by pneumofibrosis. We have examined 79 children with CNPD manifesting with symptoms of focal pneumofibrosis. The group of patients included children with congenital lung malformations (43%), consequences of bronchopulmonary dysplasia (41%), chronic bronchitis (10%), post-pneumonic pulmonary fibrosis (6%). The average age of children was 6.5±1.2 years, including 43 boys (54%) and 36 girls (46%). The comparison group included 46 children with COPD without signs of pulmonary fibrosis, the control group consisted of 30 apparently healthy children. The contents of cells with reduced MMP among lymphocytes, monocytes, and granulocytes in peripheral blood was determined with JC-1 dye, using the BD FACSCalibur instrument and Cell Quest Pro software (Becton Dickinson, USA). The proportion of lymphocytes with reduced MMP in patients with COPD was similar in the children of the main and comparison group, exceeding the indexes of the control group by 1.7 times (p < 0.001). Decreased MMP of granulocytes in children with pneumofibrosis was detected 1.9 times more often than in children with fibrosis-free CNPD cases (p < 0.05), and 3.4 times more common than in children from the control group (p < 0.001). Monocytes with reduced MMM in children with pulmonary fibrosis were detected 2 times more often than in children with COPD without fibrosis (p < 0.05), and 7.3 times more frequent than in the control group (p < 0.001). The changes were more expressed in children during exacerbation of the disease. The revealed features suggest a decreased level of metabolic activity of blood cells, thus, probably, presenting an immunopathogenetic basis for development of pneumofibrosis

Improving the efficacy of potato clonal micropropagation by inoculation with the rhizosphere bacteria <i>Azospirillum baldaniorum</i> Sp245 and <i>Ochrobactrum cytisi</i> IPA7.2

Sustainable development of agriculture depends on the provision of quality seeds to the market. Inoculation with plant-growth-promoting rhizobacteria in in vitro culture can be used to improve the growth efficacy and performance of microplants. We examined the effect of in vitro inoculation of microplants of the cultivars Nevsky and Kondor with the strains Azospirillum baldaniorum Sp245 and Ochrobactrum cytisi IPA7.2 separately and in combination. We examined the morphological variables of plant growth in in vitro culture and under ex vitro adaptation conditions; we also investigated the growth and performance of the plants in the greenhouse. The dependence of the inoculation eff icacy on potato genotype, growth stage, and inoculum composition was ascertained throughout the experiment. In vitro, A. baldaniorum Sp245 alone and in combination with O. cytisi IPA7.2 promoted the formation of roots on the microplants of both cultivars and the growth of Nevsky shoots. During plant growth ex vitro, all growth variables of the Nevsky microplants were promoted by O. cytisi IPA7.2 alone and in combination with A. baldaniorum Sp245. In both cultivars grown in the greenhouse, shoot growth was promoted in most inoculation treatments. The survival ability of the Nevsky microplants in the greenhouse increased 1.7-fold under the effect of simultaneous inoculation. Inoculation of microplants with a combination of A. baldaniorum Sp245 and O. cytisi IPA7.2 increased the number of Nevsky minitubers 1.5-fold and the number of Kondor minitubers 3.5-fold. Inoculation with the tested strains can be used to promote the growth of microplants and increase the yield of minitubers in potato seed breeding for the production of healthy planting material

Prevalence of hiv infection in one of the risk behavior groups

The article presents the results of a sentinel behavioral study among commercial sex workers in the city of Yekaterinburg. The analysis revealed the prevalence of HIV infection in this cohort and the main behavioral risks of infection, and suggested recommendations for prevention. Ключевые слова. ВИЧ-инфекция,секс-работники, рискованное поведение, дозорный надзор.В статье представлены результаты дозорного биоповеденческого исследования среди секс-работников города Екатеринбурга. В ходе анализа выявлена распространенность ВИЧ-инфекции в данной когорте и основные биоповеденческие риски инфицирования, предложены рекомендации по профилактик

Studies of Genetic Diversity of Yersinia pestis Strains Isolated in Central-Caucasian High-Mountain Natural Plague Focus

Studied have been Yersinia pestis strains isolated in Central-Caucasian high-mountain natural plague focus within the period of 1971–2012 using MLVA typing on 25 VNTR-loci. Analysis of dendrogram cluster association with certain areas, objects, and periods of the strain isolation has been carried out by means of Arc GIS 10.1 software. Established has been the connection between 25 MLVA Y. pestis types and spatial and temporal characteristics of strains isolation in the Central-Caucasian high-mountain natural plague focus, and proline requirement too. Clusters, groups and subgroups of the strains are confined to particular territories as it follows: groups are attributed to landscape-epizootiological regions, subgroups – to mountain souslik settlements. Occasionally groups of the strains disseminate onto the other landscape-epizootiological regions of the focus. Genetic variations of plague agent strains within the limits of mountain souslik settlements evolve over time which can be viewed as indirect evidence of microevolution mechanism effect

Использование дражированных семян для посева цикория корневого

Relevance. When using precision seeders, seeds that are uniform in weight and size are needed. The maximum uniformity of the seeds can be achieved by by pelleting. Thanks to the coating, small chicory seeds increase their size by 8 times or more. In this case, the composition of the shell can include various plant protection products against pathogens, growth stimulants, micronutrient fertilizers. It is advisable to seed the seeds with their initial germination of at least 95%.Material and methods. The article presents the results of a study conducted at the Rostov Vegetable Experimental Station for chicory – a branch of the Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center, where seeds with fillers were used for sowing root chicory: Maxim fungicide, treater Prestige, Isabion – liquid organic and mineral fertilizer, Agrogel.Results. As a result, a positive effect of pelleting on the sowing quality of seeds was revealed (germination energy and field germination are higher than in the control, 2% and 0.6%, respectively, and laboratory germination at the control level), effectiveness against root rot during the growing season and during winter storage time, increase in crop root crops yield by more than 30% and ensuring net profit in comparison with the control by 21.4 thousand rubles / ha. At the same time, the level of profitability is increasing by 26%. Актуальность. При использовании сеялок точного высева необходимы семена, однородные по массе и размеру. Максимальной однородности семян можно добиться при помощи дражирования. Благодаря нанесению оболочки мелкие семена цикория увеличивают свой размер в 8 раз и более. При этом в состав оболочки можно включать различные средства защиты растений от патогенов, стимуляторы роста, микроудобрения. Семена целесообразно дражировать при исходной их всхожести не ниже 95%. Инкрустатор-дражиратор ИД-10 в комплексе машин по предпосевной подготовке семян позволяет создавать многослойные оболочки на поверхности семян, не уступающие по качеству зарубежным аналогам. Большое значение имеет разработка приёмов защиты растений путём предпосевного дражирования семян с включением в состав оболочки препаратов, включающих в свой состав инсектициды, фунгициды и стимуляторы роста нового поколения.Материал и методы. В статье приведены результаты исследования, проведённого на Ростовской овощной опытной станции по цикорию – филиале ФГБНУ ФНЦО, где использовали для посева корневого цикория семена с наполнителями: фунгицид Максим – препарат контактного действия, предназначен для защиты культур от болезней, вызываемых различными видами грибов, протравливатель Престиж – обладает инсектицидным и фунгицидным действием, а также способствует более быстрому развитию растений, Изабион – жидкое органо-минеральное удобрение, Агрогель – инертный полимерный сильно набухающий при добавлении жидкости.Результаты. В результате исследований выявлено положительное влияние дражирования на посевные качества семян (энергия прорастания и полевая всхожесть выше, чем на контроле, соответственно на 2% и 0,6%, а лабораторная всхожесть на уровне контроля), эффективность против корневых гнилей в период вегетации и во время зимнего хранения, увеличение урожайности корнеплодов культуры более, чем на 30% и обеспечение чистой прибыли по сравнению с контролем на 21,4 тыс.руб/га. При этом уровень рентабельности увеличивается на 26%.