Transition on the entropic elasticity of DNA induced by intercalating molecules

We use optical tweezers to perform stretching experiments on DNA molecules when interacting with the drugs daunomycin and ethidium bromide, which intercalate the DNA molecule. These experiments are performed in the low-force regime from zero up to 2 pN. Our results show that the persistence length of the DNA-drug complexes increases strongly as the drug concentration increases up to some critical value. Above this critical value, the persistence length decreases abruptly and remains practically constant for larger drug concentrations. The contour length of the molecules increases monotonically and saturates as drugs concentration increases. Measured in- tercalants critical concentrations for the persistence length transition coincide with reported values for the helix-coil transition of DNA-drug complexes, obtained from sedimentation experiments.Comment: This experimental article shows and discuss a transition observed in the persistence length of DNA molecules when studied as a function of some intercalating drug concentrations, like daunomycin and ethidium bromide. It has 15 pages and 4 figures. The article presented here is in preprint forma

DNA-psoralen: single-molecule experiments and first principles calculations

The authors measure the persistence and contour lengths of DNA-psoralen complexes, as a function of psoralen concentration, for intercalated and crosslinked complexes. In both cases, the persistence length monotonically increases until a certain critical concentration is reached, above which it abruptly decreases and remains approximately constant. The contour length of the complexes exhibits no such discontinuous behavior. By fitting the relative increase of the contour length to the neighbor exclusion model, we obtain the exclusion number and the intrinsic intercalating constant of the psoralen-DNA interaction. Ab initio calculations are employed in order to provide an atomistic picture of these experimental findings.Comment: 9 pages, 4 figures in re-print format 3 pages, 4 figures in the published versio

Upper bound for the conductivity of nanotube networks

Films composed of nanotube networks have their conductivities regulated by the junction resistances formed between tubes. Conductivity values are enhanced by lower junction resistances but should reach a maximum that is limited by the network morphology. By considering ideal ballistic-like contacts between nanotubes we use the Kubo formalism to calculate the upper bound for the conductivity of such films and show how it depends on the nanotube concentration as well as on their aspect ratio. Highest measured conductivities reported so far are approaching this limiting value, suggesting that further progress lies with nanowires other than nanotubes.Comment: 3 pages, 1 figure. Minor changes. Accepted for publication in Applied Physics Letter

Towards absolute calibration of optical tweezers

Aiming at absolute force calibration of optical tweezers, following a critical review of proposed theoretical models, we present and test the results of MDSA (Mie-Debye-Spherical Aberration) theory, an extension of a previous (MD) model, taking account of spherical aberration at the glass/water interface. This first-principles theory is formulated entirely in terms of experimentally accessible parameters (none adjustable). Careful experimental tests of the MDSA theory, undertaken at two laboratories, with very different setups, are described. A detailed description is given of the procedures employed to measure laser beam waist, local beam power at the transparent microspheres trapped by the tweezers, microsphere radius and the trap transverse stiffness, as a function of radius and height in the (inverted microscope) sample chamber. We find generally very good agreement with MDSA theory predictions, for a wide size range, from the Rayleigh domain to large radii, including the values most often employed in practice, and at different chamber heights, both with objective overfilling and underfilling. The results asymptotically approach geometrical optics in the mean over size intervals, as they should, and this already happens for size parameters not much larger than unity. MDSA predictions for the trapping threshold, position of stiffness peak, stiffness variation with height, multiple equilibrium points and `hopping' effects among them are verified. Remaining discrepancies are ascribed to focus degradation, possibly arising from objective aberrations in the infrared, not yet included in MDSA theory.Comment: 15 pages, 20 figure

Studies of CMB structure at Dec=40. II: Analysis and cosmological interpretation

We present a detailed analysis of the cosmic microwave background structure in the Tenerife Dec=+40 degrees data. The effect of local atmospheric contributions on the derived fluctuation amplitude is considered, resulting in an improved separation of the intrinsic CMB signal from noise. Our analysis demonstrates the existence of common structure in independent data scans at 15 and 33 GHz. For the case of fluctuations described by a Gaussian auto-correlation function, a likelihood analysis of our combined results at 15 and 33 GHz implies an intrinsic rms fluctuation level of 48^{+21}_{-15} uK on a coherence scale of 4 degrees; the equivalent analysis for a Harrison-Zel'dovitch model gives a power spectrum normalisation of Q_{rms-ps} = 22^{+10}_{-6} uK. The fluctuation amplitude is seen to be consistent at the 68% confidence level with that reported for the COBE two-year data for primordial fluctuations described by a power law model with a spectral index in the range 1.0 \le n \le 1.6. This limit favours the large scale CMB anisotropy being dominated by scalar fluctuations rather than tensor modes from a gravitational wave background. The large scale Tenerife and COBE results are considered in conjunction with observational results from medium scale experiments in order to place improved limits on the fluctuation spectral index; we find n=1.10 +/- 0.10 assuming standard CDM with H_{0}=50 kms^{-1}Mpc^{-1}.Comment: 10 pages LaTeX, including 8 PostScript figures. Accepted for publication in MNRA