THE USE OF NEW REAGENT KITS FOR DETECTION AND DESCRIPTION OF ADDITIONAL ALLELES

During the screening typing of recruited volunteers with Volga Federal District for unrelated hematopoietic stem cell registry on the loci (HLA)-A, B, DRB1, DRB345 in sample No 1758 identified a new allele at locus A. The use of basic kit AlleleSEQR HLA-A Sequencing in combination with HARP – A2F98A allowed to determine the genotype of this sample – А*30:01:01, a new allele А*25, В*13, 44, DRB1*03, 09, DRB3*02, DRB4*01

ИСПОЛЬЗОВАНИЕ НОВЫХ НАБОРОВ РЕАГЕНТОВ ДЛЯ ВЫЯВЛЕНИЯ И ОПИСАНИЯ ДОПОЛНИТЕЛЬНЫХ АЛЛЕЛЕЙ

During the screening typing of recruited volunteers with Volga Federal District for unrelated hematopoietic stem cell registry on the loci (HLA)-A, B, DRB1, DRB345 in sample No 1758 identified a new allele at locus A. The use of basic kit AlleleSEQR HLA-A Sequencing in combination with HARP – A2F98A allowed to determine the genotype of this sample – А*30:01:01, a new allele А*25, В*13, 44, DRB1*03, 09, DRB3*02, DRB4*01. В ходе проведения скринингового типирования потенциальных доноров гемопоэтических стволовых клеток, проживающих на территории Приволжского федерального округа, по локусам (HLA)-A, B, DRB1, DRB345 у образца No 1758 выявлен новый аллель по локусу А. Использование базовых наборов реагентов AlleleSEQR HLA-А Sequencing в сочетании с HARP – A2F98A позволило установить генотип указанного образца – А*30:01:01, новый аллель А*25, В*13, 44, DRB1*03, 09, DRB3*02, DRB4*01.

РОЛЬ ПРЕД- И ПОСТТРАНСПЛАНТАЦИОННЫХ ФАКТОРОВ В РАЗВИТИИ КОРОНАРНОЙ БОЛЕЗНИ ТРАНСПЛАНТИРОВАННОГО СЕРДЦА

Aim: to assess the relationship between preand post-transplantation factors and degree of coronary artery lesion, reported by intravascular ultrasound study (IVUS) in patients who underwent orthotopic heart transplantation (OHT) surgery. Materials and methods. The study comprised of 27 patients who underwent OHT more than 2 years before. The age of patients was 46,8 ± 10,4 years old. All of them were preoperatively classified by HLA system. All patients received transthoracic echocardiography at terms of 1, 6, 12 and 24 months after OHT. Coronary angiography (CAG) and IVUS were performed at 24 ± 6 months. Results. In CAG none of the patients showed angiographic signs of CA stenosis, but changes of various degrees were detected by IVUS. Results obtained by IVUS were clustered to select two groups with different degree of coronary artery lesion. The donor’s age in Group 2 was evidently higher compared to Group 1 (34,77 ± 1,03 and 40,00 ± 2,04 years, respectively, p = 0,043). Donor-recipient coincidence frequency was lower in group with significant CA lesion (by 2,36, р = 0,003). The number of cardiac surgeries performed prior to OHT was higher in Group 2 (by 2,8, р = 0,008). Post-transplant factor analysis showed that the number of diabetes mellitus (DM) cases revealed after transplantation was more frequent in Group 2 (by 3,2 vs Group 1, р = 0,021). Conclusion. The degree of CA lesion according to IVUS at 24-month period after OTH was associated with several post-transplant factors, which were the presence of cardiac surgical interventions before transplantation, low HLA donor-recipient coincidence frequency, and donor’s age. The more significant CA lesion is, the more cases of DM after OHT occur. Цель: исследовать связь преди посттрансплантационных факторов со степенью поражения коронарных артерий по данным внутрисосудистого ультразвукового исследования (ВСУЗИ) у пациентов после ортотопической трансплантации сердца (ОТС). Материалы и методы. В исследование включены 27 пациентов после ОТС с давностью операции более 2 лет. Возраст пациентов составил 46,8 ± 10,4 года. Все пациенты были типированы по системе HLA перед операцией. Всем пациентам выполнялись трансторакальная эхокардиография в сроках 1, 6, 12, 24 мес. после ОТС. Коронароангиография (КАГ), ВСУЗИ проводились через 24 ± 6 мес. Результаты. При проведении КАГ у всех пациентов не выявлено ангиографических признаков стенозирования КА, но обнаружены различной степени изменения по данным ВСУЗИ. Полученные результаты ВСУЗИ подвергнуты кластеризации для выделения двух групп с различной степенью поражения коронарных артерий. Возраст донора в группе 2 был достоверно выше, чем в группе 1 (34,77 ± 1,03 и 40,00 ± 2,04 года соответственно, p = 0,043). Частота совпадений донора и реципиента меньше в группе со значимым поражением КА (в 2,36 раза, р = 0,003). Количество кардиохирургических операций, выполненных до ОТС, было больше в группе 2 (в 2,8 раза, р = 0,008). Анализ посттрансплантационных факторов показал, что случаев сахарного диабета, выявленных после трансплантации, было больше в группе 2 (в 3,2 раза по сравнению с группой 1, р = 0,021). Выводы. Степень поражения КА, по данным ВСУЗИ, через 24 месяца после ОТС ассоциирована с рядом предтрансплантационных факторов: наличием кардиохирургических вмешательств до трансплантации, низким процентом совпадений донора и реципиента по системе HLA, возрастом донора. При значимом поражении КА больше случаев развития СД после ОТС.

ROLE OF PRE- AND POST-TRANSPLANT FACTORS IN THE DEVELOPMENT OF CORONARY DISEASE OF THE TRANSPLANT HEART

Aim: to assess the relationship between preand post-transplantation factors and degree of coronary artery lesion, reported by intravascular ultrasound study (IVUS) in patients who underwent orthotopic heart transplantation (OHT) surgery. Materials and methods. The study comprised of 27 patients who underwent OHT more than 2 years before. The age of patients was 46,8 ± 10,4 years old. All of them were preoperatively classified by HLA system. All patients received transthoracic echocardiography at terms of 1, 6, 12 and 24 months after OHT. Coronary angiography (CAG) and IVUS were performed at 24 ± 6 months. Results. In CAG none of the patients showed angiographic signs of CA stenosis, but changes of various degrees were detected by IVUS. Results obtained by IVUS were clustered to select two groups with different degree of coronary artery lesion. The donor’s age in Group 2 was evidently higher compared to Group 1 (34,77 ± 1,03 and 40,00 ± 2,04 years, respectively, p = 0,043). Donor-recipient coincidence frequency was lower in group with significant CA lesion (by 2,36, р = 0,003). The number of cardiac surgeries performed prior to OHT was higher in Group 2 (by 2,8, р = 0,008). Post-transplant factor analysis showed that the number of diabetes mellitus (DM) cases revealed after transplantation was more frequent in Group 2 (by 3,2 vs Group 1, р = 0,021). Conclusion. The degree of CA lesion according to IVUS at 24-month period after OTH was associated with several post-transplant factors, which were the presence of cardiac surgical interventions before transplantation, low HLA donor-recipient coincidence frequency, and donor’s age. The more significant CA lesion is, the more cases of DM after OHT occur