Modern Technologies for Examination of Three-Dimensional (3-D) Ultra-Fine Structure and Visualization of Microorganisms

Outlined is a review of the literature data on the modern technologies for examination of the spatial (3-D) submicroscopic structural arrangement of biological objects with a high spatial resolution. Capacities of the instrumental visualization and analysis of a three-dimensional biological object significantly facilitate the overall characterization of its structural-functional properties

Examination of the Ultrastructure of Two Phenotypically Different Clones of <I>V. cholerae</I> by Means of Transmission and Scanning Electron Microscopy

Submitted are the data of examination of the ultrastructure of two phenotypically different clones (toxigenic and non-toxigenic) of V. cholerae Dakka35 strain by means of electron microscopy. Additional layer (capsule) of polysaccharide nature is identified on the surface of non-toxigenic clone bacteria. This layer (absent in toxigenic clone) alters colony morphology

Application of wavelet-analysis for image filtration in electronic and probe microscopy

The review presents the results of application of the programs of discrete and stationary wavelet-analysis for filtration of object image from noise and for the increase of its resolution capability in electronic and probe microscopy

2D effects in laminar premixed flames stabilized on a flat flame burner

In the present work non-idealities of flat burner-stabilized flames at atmospheric and low pressures are examined using PIV measurements, which are supported by and analyzed with the help of CFD modeling. Radial and axial velocity profiles measured in the cold flow and in the flames at atmospheric and at lower pressures are presented. Two possible reasons for the drop in the apparent laminar burning velocity at low pressures (below 20 kPa) were considered: the non-uniformity of the velocity profile issuing from the burner mouth and diffusion of the gas mixture from the original gas flow. At very low pressures an outward velocity at the edge of the burner plate is detected. A part of the fuel-air mixture therefore seems to escape the flow at the edge of the burner. Further experiments, however, revealed that diffusion of the fuel from the fresh mixture and of the ambient atmosphere into the flame is less important as compared to the diffusive loss of H atoms from the flame at low pressures. This was substantiated by the numerical analysis and assessment of the earlier observations from the literature. A critical relation between the size of the burner and the lowest pressure for ensuring the stabilization of a flat flame is demonstrated. (C) 2013 Elsevier Inc. All rights reserved

High-temperature oxidation of propanol isomers in the mixtures with N2O at high Ar dilution conditions

This work provides, for the first time, new information regarding the kinetics interaction between N2O and propyl alcohol isomers. To this end, the formation and consumption of atomic oxygen were measured behind the reflected shock waves using Atomic Resonance Absorption Spectroscopy (ARAS) technique for 1–10 ppm n- i-propanol + 10 ppm N2O + Ar mixtures, at 2–3 bar and over a wide temperature range of 1700–3200 K. The Konnov and POLIMI detailed combustion mechanisms were assessed against experimental data and also employed to study the main reactions influencing the oxidation dynamics of fuel mixtures under the investigated conditions. The study highlighted a certain difficulty by the models tested in predicting the formation of atomic oxygen at T < 2000 K. The rate of production and the sensitivity analysis was performed with the attempt to identify the most important reactions involved in the process oxidation for future kinetic model refinements