OPTIMIZATION OF PRESENTATION AND CONSUMER CONTAINER OF ANTI-RABIES IMMUNOGLOBULIN OBTAINED FROM HORSE SERUM

We presented the data concerning optimization of presentation and consumer container of anti-rabies immunoglobulin obtained, from horse serum.. During the experiments, ampoules and. flasks were used for primary packaging. They were filled within 5 ml of the preparation of freeze-drying. Comparative analysis of physical, chemical and. biological properties and. molecular parameters of freeze-dried and initial forms of the immunoglobulin. was carried out. Freeze-drying was demonstrated to promote stabilization of its properties and. prevent emergence of fragments and. aggregates during anti-rabies immunoglobulin storage

Determination of the Molecular Parameters of Heterologous Anti-Rabies Immunoglobulin Using Gel-Filtration

Presented are the data of analysis of molecular parameters of heterologous immunoglobulin produced by the Russian Research Anti-Plague Institute "Microbe". It is noted that the optimal system of rectification enables to obtain a preparation composed of 100 % fraction of monomers. The fraction of fragments appears in the process of immunoglobulin storage, however its content is insignificant. The fraction of aggregates is not registered at the date of preparation delivery nor after its storage during 5 years. Lyophilized preparation is determined to be advantageous in comparison with the liquid one as regards molecular parameters integrity

F(ab’)₂-Fragments of Antirabic Immunoglobulin Production Using Immobilized Pepsin

Biotechnological scheme for F(ab')2-fragments of anti-rabies immunoglobulin production using immobilized pepsin was developed. Optimal ways for immobilized pepsin production were determined, conditions for fermentative hydrolysis of anti-rabies immunoglobulin were sorted out. Protective properties of F(ab')2-fragments of anti-rabies immunoglobulin produced by means of this method were assessed

Production of Lyophilized Preparation of Anti-Rabies Immunoglobulin and Examination of Its Main Properties

Presented are data on examination of physical, chemical and biological characteristics of lyophilized preparation of geterologous anti-rabies immunoglobulin. Dry and original liquid forms of immunoglobulin were comparatively analyzed. The lyophilized form was shown to preserve main properties during long-term storage