Identification and characterization of pathogenic viruses in aquatic animal using advanced techniques in order to develop rapid diagnostic kit (shrimp viral disease kit as well as TSV, HPV and MBV )

Shrimp production increasing rapidly in the world and in2013 the production reaches 4.2 MT. In Iran the shrimp production is under development and estimated in 1393, 20 thousand tons produced. In this regards the important subject is health and disease in shrimp farm. The white spot syndrome virus for second time appears in chabahar and damage many farms. Because the aquaculture activity expand in the world in national, regional and international scale, many emerge disease are endanger. In this regard the viral disease is very important and not only decrease the production but also has a side effect in business and national economy. For control and prevention the viral disease, the accurate methods such as PCR kit were developed. In this project the PCR methods with sensitivity, specificity and efficacy was designed and used for detection viral disease. Many viruses have several serotypes and in different area maybe new serotype induce the disease. For this reason, the specific kit will be design. Three viruses consist of MBV, TSV and IHHNV are very pathogenic in shrimp farm and need the specific PCR kit for detection them. In this project the MBV virus was identified and designs a new primer with Oligo software and the primer amplified a part of DNA with 185 bp in the gel. The specificity and sensitivity of primer were checked by IQ2000 Kit and the primer used for detection unknown samples

Design of current mode pi controller for single-phase pwm inverter

This paper presents the design of current mode PI controller for single-phase PWM inverter. The controller is comprised of inductor current as the inner loop and output voltage as the outer feedback loop. The control design is carried out using Sisotool, which is provided in Matlab. By using this tool, users can examine the effects of changing the gain control values to system's transient response and stability, simultaneously. Hence, simplify and speed up the control design process. To evaluate the performance of the designed controller, the inverter is simulated under several types of load disturbances. From the result, it is shown that the designed controller exhibits a good transient response i.e. fast rising and settling time with small overshoot when subjected to step load disturbance. © 2011 IEEE