Management of myeloperoxidase activity of leukocytes in those suffering from community acquired pneumonia with the help of low-intesive lazer radiation of blood

Goal of the study: to investigate the effect of low-intense laser radiation of blood on the level myeloperoxidase in those suffering from community-acquired pneumonia.Materials and methods. 78 patients were enrolled into the study, 52 of them received the intravenous laser radiation of blood (VLOK) as per VLOK-405 technique during 7 days. The level of myeloperoxidase was tested with the help of reagents kit for blood enzyme multiplied immunoassay before and after the treatment.Results. The analysis of the study results detected the confident improvement of myeloperoxidase rates in the group of patients receiving additional VLOK treatment. Conclusions. Using VLOK as a part of integral therapy of those suffering from community-acquired pneumonia promotes the normalization of myeloperoxidase rates

ВЛИЯНИЕ ЛАЗЕРНОГО ОБЛУЧЕНИЯ НА УРОВЕНЬ СОДЕРЖАНИЯ АДЕНОЗИНДЕЗАМИНАЗЫ В ПЛЕВРАЛЬНОЙ ЖИДКОСТИ

The aim of this study was to investigate effects of low-intense laser irradiation (intravenous, intrapleural and combined) on adenosine deaminase (ADA) concentration in the pleural fluid of patients with pleural effusion of various etiology.Materials and methods. We examined patients aged 30 to 80 years with pleural effusion (n = 45; 25 males (55.6 %), 20 females (44.4 %); the mean age, 67.6 ± 13.9 years). The cause of the pleural effusion was pneumonia (n = 14), trauma (n = 2) or chronic heart failure (n = 29). All patients were randomly divided in a control group (n = 17) or laser treatment group (n = 28). The control group patients received standard treatment according to etiology of the effusion. The laser group patients were treated with laser and medications. Laser was applied using "Matrix-VLOK" device (Matrix, Russia) with the wave length of 0.365 μm and output of 1.0 – 1.5 mWt. Laser therapy was administered in continuous irradiation mode; course duration was 3 to 7 sessions in dependence on a rate of the fluid accumulation in the pleural cavity, in every other day. Intravenous laser irradiation was performed for 10 min after cubital vena puncture with a needle with KIVL-01 light-guide fiber. Intrapleural laser irradiation was performed for 10 min after pleurocentesis with the needle and light-guide fiber following pleural fluid suction and instillation of a wide-spectrum antibiotic solution. The pleural fluid was centrifuged during 10 min in 1,500 g and then was freezed in –20 °С until the examination. Studied parameters were measured before and after the treatment.Results. The laser group patients demonstrated a significant decrease in ADA concentration compared to baseline (25.30 ± 1.56 and 18.70±1.94МЕ × l–1 before and after treatment, respectively). The laser therapy in addition to the standard treatment was also related to clinical improvement. In controls the standard medication therapy did not lead to any change in ADA concentration (25.70 ± 1.63 and 22.80 ± 1.98 МЕ × l–1 before and after treatment, respectively).Conclusion. The results showed that in patients with pleural effusion the laser therapy in addition to the standard treatment was related to decrease in ADA concentration, slower fluid accumulation in the pleural cavity and shorter treatment duration. Time to the second pleural puncture was longer, the need in drug therapy was diminished and the treatment efficacy was higher in the laser treatment group. These effects led to improving quality of life and was thought to make the laser therapy rational in patients with pleural effusion of various etiology.Изучалось влияние низкоинтенсивного лазерного излучения (внутривенного, внутриплеврального и комбинированного) на динамику уровня аденозиндезаминазы (АДА) в плевральной жидкости у больных с плевральным выпотом различной этиологии. Обследованы больные с плевральным выпотом в возрасте от 30 до 80 лет (n = 45: 25 (55,6 %) мужчин, 20 (44,4 %) женщин; средний возраст 67,6 ± 13,9 года). Выявлены парапневмонический (n = 14) и посттравматический (n = 2) плеврит, а также плевральный выпот как проявление хронической сердечной недостаточности (n = 29).Все больные случайным методом были распределены в 2 группы: контрольную составили пациенты (n = 17), которые получали медикаментозное лечение в зависимости от этиологии плеврита; пациенты основной группы (n = 28) дополнительно к медикаментозному лечению получали лазерную терапию.Лазерная терапия осуществлялась при помощи аппарата "Матрикс-ВЛОК" ("Матрикс", Россия); длина волны – 0,365 мкм, выходная мощность – 1,0–1,5 мВт. Лазерное облучение проводилось в непрерывном режиме излучения, курс лечения зависел от скорости накопления жидкости в плевральной полости и составлял от 3 до 5–7 процедур через день.Внутривенное лазерное облучение крови проводилось в течение 10 мин путем венопункции локтевой вены одноразовой иглой со световодом КИВЛ-01. Внутриплевральное облучение проводилось путем плевроцентеза. После прокола плевральной полости и получения плевральной жидкости вводилась игла со световодом и проводилось внутриплевральное облучение в течение 10 мин. После облучения жидкость эвакуировалась, а в полость вводился антибактериальный препарат широкого спектра действия. Образцы биологического материала (плевральной жидкости), полученные путем пункции, центрифугировались в течение 10 мин при 1 500 g и замораживались однократно при –20 °С до непосредственного исследования. Исследуемый показатель определялся до и после лечения. У больных основной группы, которым проводились сеансы лазерной терапии, отмечено достоверное снижение содержания АДА по сравнению с исходными данными (25,30 ± 1,56 и 18,70 ± 1,94 МЕ / л соответственно до и после лечения); в контрольной группе традиционная медикаментозная терапия не сопровождалась изменением содержания АДА (25,70 ± 1,63 и 22,80 ± 1,98 МЕ / л соответственно до и после лечения). Включение лазерной терапии в комплексное лечение сопровождалось также улучшением клинического течения заболевания.Показано, что при включении в комплексную терапию больных с плевральным выпотом различной этиологии лазерного облучения снижается уровень АДА, отмечается сокращение скорости накопления жидкости и сроков лечения заболевания, увеличивается время между плевральными пункциями, снижается медикаментозная нагрузка на больного, что свидетельствует об улучшении качества жизни и эффективности данного лечения

Коррекция миелопероксидазной активности лейкоцитов у больных внебольничной пневмонией с помощью низкоинтенсивного лазерного облучения крови

Goal of the study: to investigate the effect of low-intense laser radiation of blood on the level myeloperoxidase in those suffering from community-acquired pneumonia.Materials and methods. 78 patients were enrolled into the study, 52 of them received the intravenous laser radiation of blood (VLOK) as per VLOK-405 technique during 7 days. The level of myeloperoxidase was tested with the help of reagents kit for blood enzyme multiplied immunoassay before and after the treatment.Results. The analysis of the study results detected the confident improvement of myeloperoxidase rates in the group of patients receiving additional VLOK treatment. Conclusions. Using VLOK as a part of integral therapy of those suffering from community-acquired pneumonia promotes the normalization of myeloperoxidase rates. Цель исследования: изучение влияния низкоинтенсивного лазерного облучения крови на уровень миелопероксидазы у больных внебольничной пневмонией.Материалы и методы. В исследование включено 78 пациентов, 52 из которых получали процедуры внутривенного лазерного облучения крови (ВЛОК) по методике ВЛОК-405 в течение 7 дней. Уровень миелопероксидазы определяли с помощью набора реагентов для иммуноферментного анализа крови до и после лечения.Результаты. При анализе результатов исследования выявлено достоверное улучшение показателей миелопероксидазы в группе больных, получавших дополнительно процедуры ВЛОК.Выводы. Использование ВЛОК в комплексной терапии больных внебольничной пневмонией способствует нормализации показателей миелопероксидазы

Коррекция эндотелиальной дисфункции у больных внебольничной пневмонией с помощью низкоинтенсивного лазерного облучения крови

The aim of the study was to investigate effects of low-level laser blood irradiation on nitric oxide (NO) in patients with community-acquired pneumonia (CAP). Methods. The patients were divided into two groups: the control group receiving medication treatment only and the study group additionally receiving low-level laser blood irradiation (LLBI). NO was detected as a sum of its end products using immunoenzyme assay. Results. A significant improvement in NO level after LLBI was seen both in patients with baseline increased or decreased concentration of NO metabolites. Conclusion. LLBI in patients with community-acquired pneumonia was accompanied by improvement of endothelial function.Цель. Изучение влияния низкоинтенсивного лазерного излучения на уровень оксида азота (NO) в крови у больных внебольничной пневмонией (ВП). Материалы и методы. Больные были распределены на 2 группы: контрольную, пациенты которой получали только медикаментозную терапию и основную, где дополнительно проводились курсы внутривенного лазерного облучения крови (ВЛОК) по методике ВЛОК-405. Уровень NO определялся по сумме его конечных метаболитов при помощи набора реагентов для иммуноферментного анализа. Результаты. При анализе полученных данных выявлено достоверное улучшение показателей NO как при исходно повышенном, так при пониженном содержании его метаболитов у больных, получавших дополнительно лазерную терапию. Заключение. Включение ВЛОК в комплексную терапию больных ВП сопровождается нормализацией показателей функции эндотелия

Predictors of enhancing human physical attractiveness: Data from 93 countries

People across the world and throughout history have gone to great lengths to enhance their physical appearance. Evolutionary psychologists and ethologists have largely attempted to explain this phenomenon via mating preferences and strategies. Here, we test one of the most popular evolutionary hypotheses for beauty-enhancing behaviors, drawn from mating market and parasite stress perspectives, in a large cross-cultural sample. We also test hypotheses drawn from other influential and non-mutually exclusive theoretical frameworks, from biosocial role theory to a cultural media perspective. Survey data from 93,158 human participants across 93 countries provide evidence that behaviors such as applying makeup or using other cosmetics, hair grooming, clothing style, caring for body hygiene, and exercising or following a specific diet for the specific purpose of improving ones physical attractiveness, are universal. Indeed, 99% of participants reported spending >10 min a day performing beauty-enhancing behaviors. The results largely support evolutionary hypotheses: more time was spent enhancing beauty by women (almost 4 h a day, on average) than by men (3.6 h a day), by the youngest participants (and contrary to predictions, also the oldest), by those with a relatively more severe history of infectious diseases, and by participants currently dating compared to those in established relationships. The strongest predictor of attractiveness-enhancing behaviors was social media usage. Other predictors, in order of effect size, included adhering to traditional gender roles, residing in countries with less gender equality, considering oneself as highly attractive or, conversely, highly unattractive, TV watching time, higher socioeconomic status, right-wing political beliefs, a lower level of education, and personal individualistic attitudes. This study provides novel insight into universal beauty-enhancing behaviors by unifying evolutionary theory with several other complementary perspectives

Low-intensity laser radiation in microcirculation correction among patients with arterial hypertension

Aim. To investigate the effects of intravenous blood laser radiation (IBLR) on microcirculation (MC) in patients with arterial hypertension (AH).Material and methods. The study included 120 patients (mean age 53,4±1,3 years) with Stage I-II AH. All participants were divided into two groups: the main group (MG) received pharmaceutical therapy plus IBLR, the control group (CG) was administered pharmaceutical therapy only. MC was assessed by laser Doppler flowmetry (LDF) method with LAKK-02 device.Results. AH patients demonstrated heterogeneity of hemodynamic MC types (HMCT), with higher prevalence of pathological types: congestive-static, spastic and hyperemic HMCT. IBLR addition to complex AH treatment was associated with significant MC improvement, regardless of the initial HMCT — mostly due to reduced spasm of afferent vessels.Conclusion. Comparing the MG and CG results, it could be assumed that IBLR is an effective method for MC correction in AH patients. These MC disturbances are linked to AH progression and high blood pressure stabilization

Predictors of enhancing human physical attractiveness: Data from 93 countries

People across the world and throughout history have gone to great lengths to enhance their physical appearance. Evolutionary psychologists and ethologists have largely attempted to explain this phenomenon via mating preferences and strategies. Here, we test one of the most popular evolutionary hypotheses for beauty-enhancing behaviors, drawn from mating market and parasite stress perspectives, in a large cross-cultural sample. We also test hypotheses drawn from other influential and non-mutually exclusive theoretical frameworks, from biosocial role theory to a cultural media perspective. Survey data from 93,158 human participants across 93 countries provide evidence that behaviors such as applying makeup or using other cosmetics, hair grooming, clothing style, caring for body hygiene, and exercising or following a specific diet for the specific purpose of improving ones physical attractiveness, are universal. Indeed, 99% of participants reported spending >10 min a day performing beauty-enhancing behaviors. The results largely support evolutionary hypotheses: more time was spent enhancing beauty by women (almost 4 h a day, on average) than by men (3.6 h a day), by the youngest participants (and contrary to predictions, also the oldest), by those with a relatively more severe history of infectious diseases, and by participants currently dating compared to those in established relationships. The strongest predictor of attractiveness-enhancing behaviors was social media usage. Other predictors, in order of effect size, included adhering to traditional gender roles, residing in countries with less gender equality, considering oneself as highly attractive or, conversely, highly unattractive, TV watching time, higher socioeconomic status, rightwing political beliefs, a lower level of education, and personal individualistic attitudes. This study provides novel insight into universal beauty-enhancing behaviors by unifying evolutionary theory with several other complementary perspectives